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http://dx.doi.org/10.5657/fas.2007.10.1.016

Characterization of a Myostatin-like Gene from the Scallop Patinopecten yessoensis  

Kim, Hyun-Woo (Department of Marine Biology, Pukyong National University)
Kim, Hak-Jun (Korea Polar Research Institute, Songdo Techno Park)
Yoo, Myong-Suk (Department of Marine Biology, Pukyong National University)
Publication Information
Fisheries and Aquatic Sciences / v.10, no.1, 2007 , pp. 16-23 More about this Journal
Abstract
Myostatin (GDF8) is a growth factor that limits muscle tissue growth and development in vertebrates. We isolated a myostatin-like gene (Py-MSTN) from the marine invertebrate, the scallop Patinopecten yessoensis. Py-MSTN was highly expressed in the adductor muscle and in the gill unexpectedly. Amino acid analysis showed that Py-MSTN has 49% amino acid sequence identity and 64% similarity to human myostatin (Hs-MSTN), and 42% identity and 61% similarity to myoglianin, the only invertebrate homolog. These results indicated that Py-MSTN may be functionally similar to the vertebrate MSTN than the invertebrate homolog. Phylogenetic analysis suggested that Py-MSTN is an ancestral form of vertebrate MSTN and GDF11 and does not belong to other $TGF-{\beta}$ family members. Molecular modeling showed that Py-MSTN exhibits a similar tertiary structure to mammalian BMP7, a member of $TGF-{\beta}$ family. In addition, the amino acid residues which contact extracellular domain of the receptor were relavively conserved. Given these results, we propose that Py-MSTN is a functionally active member of the $TGF-{\beta}$ family and is involved In muscle growth and regulation.
Keywords
Patinopecten yessoensis; Scallop; Myostatin; $TGF-{\beta}$; Muscle;
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