[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.5657/fas.2006.9.2.057

Optimal Culture Conditions and Isolation of a ι-Carrageenase-producing Marine Bacterium

Shim Hang-Sun (Division of Food Science and Biotechnology, Pukyong National University)
Lim Su-Jin (Division of Food Science and Biotechnology, Pukyong National University)
Choi Min-Jung (Division of Food Science and Biotechnology, Pukyong National University)
Kim Jong-Oh (EnbioGene Co. Ltd., Department of Food and Life Science, Pukyong National University)
Kim Seok-Ryel (EnbioGene Co. Ltd., Department of Food and Life Science, Pukyong National University)
Kim Hyeung-Rak (Division of Food Science and Biotechnology, Pukyong National University)

Publication Information

Fisheries and Aquatic Sciences / v.9, no.2, 2006 , pp. 57-63 More about this Journal

Abstract

A bacterial strain capable of hydrolyzing carrageenan was isolated from the coast of Busan in Korea. The isolated strain (HS5322) is aerobic, gram-negative, rod-shaped, and motile. Comparison of the 16S rDNA of the isolate with that of known Pseudomonas sp. showed that sequence similarity was at most 95%, implying that the isolate is a new Pseudomonas species. The organism grew optimally at NaCl concentrations of 2.0 to 2.5%. The optimum temperature and pH for carrageenase production in a 72-h flask culture containing 1% carrageenan was $20^{\circ}C$ and pH 8.5, respectively. Of the synthetic substrates tested, the highest enzyme activity was obtained with p-nitrophenyl ${\beta}$ -D-galactopyranoside.

Keywords

Carrageenan; Carrageenase; Pseudomonas HS5322;

Citations & Related Records

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