Real Time Measurement of Protease Activity of Live Uronema marinum (Ciliata: Scuticociliatida) by Fluorescence Polarization Assay |
Lee Eun Hye
(Department of Aquatic Life Medicine, Pukyong National University)
Kwon Se Ryun (Department of Aquatic Life Medicine, Pukyong National University) Kim Chun Soo (Department of Aquatic Life Medicine, Pukyong National University) Chung Joon Ki (Department of Aquatic Life Medicine, Pukyong National University) Lee Hyung Ho (Faculty of Food Science and Biotechnology, Pukyong National University) Kim Ki Hong (Department of Aquatic Life Medicine, Pukyong National University) |
1 | Thompson, C.L. Jr. and L. Moewus. 1964. Miamiensis avidus n.g.n.s., a marine facultative parasite in the ciliate order Hymenostomatida. J. Protozool., 11, 378-381 DOI |
2 | Yoshinaga, T. and J. Nakazoe. 1993. Isolation and in vitro cultivation of an unidentified ciliate causing scuticociliatosis in Japanese flounder (Paralichthys oliveceus). Gyobyo Kenkyu, 28, 131-134 DOI |
3 | Gill, P.A. an d R.B. Calinan. 1997. Ulcerative dermatitis associated with Uronema sp. Infection of farmed sand whiting Sillago ciliata. Aust. Vet. J., 75, 357 DOI ScienceOn |
4 | Iglesias, R., A. Parama, M.F. Alvarez, J. Leiro, J. Fernandez and M.L. Sanmartin. 2001. Philasterides dicentrarchi (Ciliophora, Scuticociliatida) as the causative agent of scuticociliatosis in farmed turbot Scophthalmus maximus in Galicia (NW Spain). Dis. Aquat. Org., 46, 47-55 DOI |
5 | Jee, B.Y., Y.C. Kim and M.S. Park. 2001. Morphology and biology of parasite responsible for scuticociliatosis of cultured olive flounder Paralichthys olivaceus. Dis. Aquat. Org., 47. 49-55 DOI ScienceOn |
6 | McKerrow, J.H. 1989. Minireview: Parasite proteases. Expl. Parasitol., 68, 111-115 DOI ScienceOn |
7 | Mckerrow, J.H., E. Sun, P.J. Rosenthal and J. Bouvier. 1993. The proteases and pathogenicity of parasitic protozoa. Ann. Rev. Microbiol., 47, 821-853 DOI ScienceOn |
8 | Munday, B.L., P.J. O'Donoghue, M. Watts, K. Rough and T. Hawkesford. 1997. Fatal encephalitis due to the scuticociliate Uronema nigricans in sea-caged, southern bluefin tuna Thunnus maccoyii. Dis. Aquat. Org., 30, 17-25 DOI |
9 |
Schade, S.Z., M.E. Jolley, B.J. Sarauer and L.G. Simonson. 1996. BODIPY- |
10 | Sterud, E., M.K. Hansen and T.A. Mo. 2000. Systemic infection with Uronema-like ciliates in farmed turbot, Scophtalmus maximus (L.). J. Fish Dis., 23, 33-37 DOI ScienceOn |
11 | Dykova, I. and A. Figueras. 1994. Histopathological changes in turbo: Scophthalmus maximus due to a histophagous ciliate. Dis. Aquat. Org., 18, 5-9 DOI ScienceOn |
12 | Bolger, R. and W. Checovich. 1994. A new protease activity assay using fluorescence polarization. Biotechniques, 17, 585-589 |
13 | Cheung, P.J., R.F. Nigrelli and G.D. Ruggieri. 1980. Studies on the morphology of Uronema marinum Dujardin (Ciliatea: Uronematidae) with a description of the histopatology of the infection in marine fishes. J. Fish Dis., 3, 295-303 DOI |
14 | Dragesco, A., J. Dragesco, F. Coste, C. Gasc, B. Romestand, J. Raymond and G. Bouix. 1995. Philasterides dicentrarchi, n. sp. (Ciliophora, Scuticociliatida), a histophagous opportunistic parasite of Dicentrachus labrax (Linnaeus, 17 58), a reared marine fish. Eur. J. Protistol., 31, 327-340 DOI ScienceOn |
![]() |