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http://dx.doi.org/10.4491/eer.2018.141

Nitrogen removal, nitrous oxide emission and microbial community in sequencing batch and continuous-flow intermittent aeration processes  

Sun, Yuepeng (Guangdong Province Engineering Research Center for Urban Water Recycling and Environmental Safety, Graduate School at Shenzhen, Tsinghua University)
Xin, Liwei (Guangdong Province Engineering Research Center for Urban Water Recycling and Environmental Safety, Graduate School at Shenzhen, Tsinghua University)
Wu, Guangxue (Guangdong Province Engineering Research Center for Urban Water Recycling and Environmental Safety, Graduate School at Shenzhen, Tsinghua University)
Guan, Yuntao (Guangdong Province Engineering Research Center for Urban Water Recycling and Environmental Safety, Graduate School at Shenzhen, Tsinghua University)
Publication Information
Environmental Engineering Research / v.24, no.1, 2019 , pp. 107-116 More about this Journal
Abstract
Nitrogen removal, nitrous oxide ($N_2O$) emission and microbial community in sequencing batch and continuous-flow intermittent aeration processes were investigated. Two sequencing batch reactors (SBRs) and two continuous-flow multiple anoxic and aerobic reactors (CMRs) were operated under high dissolved oxygen (DO) (SBR-H and CMR-H) and low DO (SBR-L and CMR-L) concentrations, respectively. Nitrogen removal was enhanced under CMR and low DO conditions (CMR-L). The highest total inorganic nitrogen removal efficiency of 91.5% was achieved. Higher nitrifying and denitrifying activities in SBRs were observed. CMRs possessed higher $N_2O$ emission factors during nitrification in the presence of organics, with the highest $N_2O$ emission factor of 60.7% in CMR-L. SBR and low DO conditions promoted $N_2O$ emission during denitrification. CMR systems had higher microbial diversity. Candidatus Accumulibacter, Nitrosomonadaceae and putative denitrifiers ($N_2O$ reducers and producers) were responsible for $N_2O$ emission.
Keywords
Continuous-flow multiple anoxic and aerobic reactor; Intermittent aeration process; Nitrogen removal; Nitrous oxide; Sequencing batch reactor;
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1 Gong YK, Peng YZ, Yang Q, Wu WM, Wang SY. Formation of nitrous oxide in a gradient of oxygenation and nitrogen loading rate during denitrification of nitrite and nitrate. J. Hazard. Mater. 2012;227-228:453-460.   DOI
2 Smolders GJF, Van der Meij J, Van Loosdrecht MCM, Heijnen JJ. Model of the anaerobic metabolism of the biological phosphorus removal process: Stoichiometry and pH influence. Biotechnol. Bioeng. 1994;43:461-470.   DOI
3 APHA. Standard methods for the examination of water and wastewater. Washington: American Public Health Association;1995.
4 Kimochi Y, Inamori Y, Mizuochi M, Xu KQ, Matsumura M. Nitrogen removal and $N_2O$ emission in a full-scale domestic wastewater treatment plant with intermittent aeration. J. Ferment. Bioeng. 1998;86:202-206.   DOI
5 Caporaso JG, Lauber CL, Walters WA, et al. Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample. Proc. Natl. Acad. Sci. USA 2011;108:4516-4522.   DOI
6 Liu Y, Shi H, Xia L, et al. Study of operational conditions of simultaneous nitrification and denitrification in a carrousel oxidation ditch for domestic wastewater treatment. Bioresour. Technol. 2010;101:901-906.   DOI
7 Chen AC, Chang JS, Yang L, Yang YH. Nitrogen removal from sewage by continuous flow SBR system with intermittent aeration. Environ. Technol. 2001;22:553-559.   DOI
8 Mosquera-Corral A, Gonzalez F, Campos JL, Mendez R. Partial nitrification in a SHARON reactor in the presence of salts and organic carbon compounds. Process Biochem. 2005;40:3109-3118.   DOI
9 Sun Y, Guan Y, Wang D, Liang K, Wu G. Potential roles of acyl homoserine lactone based quorum sensing in sequencing batch nitrifying biofilm reactors with or without the addition of organic carbon. Bioresour. Technol. 2018;259:136-145.   DOI
10 Takeda M, Yoneya A, Miyazaki Y, et al. Prosthecobacter fluviatilis sp. nov., which lacks the bacterial tubulin btubA and btubB genes. Int. J. Syst. Evol. Microbiol. 2008;58:1561-1565.   DOI
11 Shapleigh JP. The denitrifying prokaryotes. The Prokaryotes. 2006. p. 769-792.
12 Sun Y, Guan Y, Pan M, Zhan X, Hu Z, Wu G. Enhanced biological nitrogen removal and $N_2O$ emission characteristics of the intermittent aeration activated sludge process. Rev. Environ. Sci. Bio/Technol. 2017;16:1-20.   DOI
13 Wang H, Guan Y, Li L, Wu G. Characteristics of biological nitrogen removal in a multiple anoxic and aerobic biological nutrient removal process. Biomed Res. Int. 2015;2015:531015.   DOI
14 Winkler MH, Boets P, Hahne B, Goethals P, Volcke EI. Effect of the dilution rate on microbial competition: r-strategist can win over k-strategist at low substrate concentration. Plos One 2017;12:e0172785.   DOI
15 Park HD, Noguera DR. Nitrospira community composition in nitrifying reactors operated with two different dissolved oxygen levels. J. Microbiol. Biotechnol. 2008;18:1470-1474.
16 Chain P, Lamerdin J, Larimer F, et al. Complete genome sequence of the ammonia-oxidizing bacterium and obligate chemolithoautotroph Nitrosomonas europaea. J. Bacteriol. 2003;185:2759-2773.   DOI
17 Lu H, Chandran K, Stensel D. Microbial ecology of denitrification in biological wastewater treatment. Water Res. 2014;64:237-254.   DOI
18 Zheng M, Tian Y, Liu T, et al. Minimization of nitrous oxide emission in a pilot-scale oxidation ditch: generation, spatial variation and microbial interpretation. Bioresour. Technol. 2015;179:510-517.   DOI
19 Etchebehere C, Errazquin MI, Dabert P, Moletta R, Muxi L. Comamonas nitrativorans sp. nov., a novel denitrifier isolated from a denitrifying reactor treating landfill leachate. Int. J. Syst. Evol. Microbiol. 2001;51:977-983.   DOI
20 Yoon H, Song MJ, Yoon S. Design and feasibility analysis of a self-sustaining biofiltration system for removal of low concentration $N_2O$ emitted from wastewater treatment plants. Environ. Sci. Technol. 2017;51:10736-10745.   DOI
21 Peng L, Ni BJ, Ye L, Yuan Z. The combined effect of dissolved oxygen and nitrite on $N_2O$ production by ammonia oxidizing bacteria in an enriched nitrifying sludge. Water Res. 2015;73:29-36.   DOI
22 Ge S, Peng Y, Qiu S, Zhu A, Ren N. Complete nitrogen removal from municipal wastewater via partial nitrification by appropriately alternating anoxic/aerobic conditions in a continuous plug-flow step feed process. Water Res. 2014;55:95-105   DOI
23 Gilbert EM, Agrawal S, Brunner F, Schwartz T, Horn H, Lackner S. Response of different Nitrospira species to anoxic periods depends on operational DO. Environ. Sci. Technol. 2014;48:2934-2941.   DOI
24 Wunderlin P, Mohn J, Joss A, Emmenegger L, Siegrist H. Mechanisms of $N_2O$ production in biological wastewater treatment under nitrifying and denitrifying conditions. Water Res. 2012;46:1027-1037.   DOI
25 Tallec G, Garnier J, Billen G, Gousailles, M. Nitrous oxide emissions from denitrifying activated sludge of urban wastewater treatment plants, under anoxia and low oxygenation. Bioresour. Technol. 2008;99:2200-2209.   DOI
26 Pan M, Wen X, Wu G, Zhang M, Zhan X. Characteristics of nitrous oxide ($N_2O$) emission from intermittently aerated sequencing batch reactors (IASBRs) treating slaughterhouse wastewater at low temperature. Biochem. Eng. J. 2014;86:62-68.   DOI
27 Shen L, Guan Y, Wu G. Effect of heterotrophic activities on nitrous oxide emission during nitrification under different aeration rates. Desalination. Water Treat. 2015;55:821-827.   DOI
28 Wang H, Sun Y, Wu G, Guan Y. Effect of anoxic to aerobic duration ratios on nitrogen removal and nitrous oxide emission in the multiple anoxic/aerobic process. Environ. Technol. 2018; doi.org/10.1080/09593330.2018.1427801.   DOI
29 Gabarro J, Gonzalez-Carcamo P, Ruscalleda M, et al. Anoxic phases are the main $N_2O$ contributor in partial nitritation reactors treating high nitrogen loads with alternate aeration. Bioresour. Technol. 2014;163:92-99.   DOI
30 Wang Q, Jiang G, Ye L, Pijuan M, Yuan Z. Heterotrophic denitrification plays an important role in $N_2O$ production from nitritation reactors treating anaerobic sludge digestion liquor. Water Res. 2014;62:202-210.   DOI
31 Ciggin AS, Rossetti S, Majone M, Orhon D. Effect of feeding and sludge age on acclimated bacterial community and fate of slowly biodegradable substrate. Bioresour. Technol. 2011;102:7794-7801.   DOI
32 Sheng X, Liu R, Song X, Chen L, Tomoki K. Comparative study on microbial community in intermittently aerated sequencing batch reactors (SBR) and a traditional SBR treating digested piggery wastewater. Front. Environ. Sci. Eng. 2017;11:97-103.
33 Jia W, Zhang J, Xie H, et al. Effect of PHB and oxygen uptake rate on nitrous oxide emission during simultaneous nitrification denitrification process. Bioresour. Technol. 2012;113:232-238.   DOI
34 Sun Y, Wang H, Wu G, Guan Y. Nitrogen removal and nitrous oxide emission from a step-feeding multiple anoxic and aerobic process. Environ. Technol. 2017;39:814-823.
35 Sims A, Gajaraj S, Hu Z. Nutrient removal and greenhouse gas emissions in duckweed treatment ponds. Water Res. 2013;47:1390-1398.   DOI
36 Terada A, Sugawara S, Yamamoto T, Zhou S, Koba K, Hosomi M. Physiological characteristics of predominant ammonia-oxidizing bacteria enriched from bioreactors with different influent supply regimes. Biochem. Eng. J. 2013;79:153-161.   DOI
37 Liang W, Chao Y, Ren H, Geng J, Ding L, Ke X. Minimization of nitrous oxide emission from CASS process treating low carbon source domestic wastewater: Effect of feeding strategy and aeration rate. Bioresour. Technol. 2015;198:172-180.   DOI
38 Lyu W, Huang L, Xiao G, Chen Y. Effects of carbon sources and cod/n ratio on $N_2O$ emissions in subsurface flow constructed wetlands. Bioresour. Technol. 2017;245:171-181.   DOI
39 Strand SE, Mcdonnell AJ, Unz RF. Oxygen and nitrate reduction kinetics of a nonflocculating strain of Zoogloea ramigera. Antonie Van Leeuwenhoek 1998;54:245-255.   DOI
40 Kim DJ, Kim SH. Effect of nitrite concentration on the distribution and competition of nitrite-oxidizing bacteria in nitratation reactor systems and their kinetic characteristics. Water Res. 2006;40:887-894.   DOI
41 Chandran K, Stein LY, Klotz MG, van Loosdrecht MC. Nitrous oxide production by lithotrophic ammonia-oxidizing bacteria and implications for engineered nitrogen-removal systems. Biochem. Soc. Trans. 2011;39:1832-1837.   DOI
42 Pijuan M, Tora J, Rodriguezcaballero A, Cesar E, Carrera J, Perez J. Effect of process parameters and operational mode on nitrous oxide emissions from a nitritation reactor treating reject wastewater. Water Res. 2014;49:23-33.   DOI
43 Wang H, Guan Y, Pan M, Wu G. Aerobic $N_2O$ emission for activated sludge acclimated under different aeration rates in the multiple anoxic and aerobic process. J. Environ. Sci. 2016;43:70-79.   DOI
44 Feng S, Tan CH, Constancias F, Kohli GS, Cohen Y, Rice SA. Predation by Bdellovibrio bacteriovorus significantly reduces viability and alters the microbial community composition of activated sludge flocs and granules. FEMS Microbiol. Ecol. 2017;93.
45 Lemaire R, Meyer R, Taske A, Crocetti GR, Keller J, Yuan Z. Identifying causes for $N_2O$ accumulation in a lab-scale sequencing batch reactor performing simultaneous nitrification, denitrification and phosphorus removal. J. Biotechnol. 2006;122:62-72.   DOI
46 Zhou Y, Pijuan M, Zeng RJ, Yuan Z. Free nitrous acid inhibition on nitrous oxide reduction by a denitrifying-enhanced biological phosphorus removal sludge. Environ. Sci. Technol. 2008;42:8260-8265.   DOI
47 Zeng RJ, Lemaire R, Yuan Z, Keller J. Simultaneous nitrification, denitrification, and phosphorus removal in a lab-scale sequencing batch reactor. Biotechnol. Bioeng. 2003;84:170-178.   DOI
48 Kim JM, Lee HJ, Kim SY, Song JJ, Park W, Jeon CO. Analysis of the fine-scale population structure of "Candidatus Accumulibacter phosphatis" in enhanced biological phosphorus removal sludge, using fluorescence in situ hybridization and flow cytometric sorting. Appl. Environ. Microbiol. 2010;76:3825-3835.   DOI
49 Lv XM, Shao MF, Li J, Li CL. Metagenomic analysis of the sludge microbial community in a lab-scale denitrifying phosphorus removal reactor. Appl. Biochem. Biotechnol. 2015;175:3258-3270.   DOI
50 Yang C, Zhang W, Liu R, et al. Phylogenetic diversity and metabolic potential of activated sludge microbial communities in full-scale wastewater treatment plants. Environ. Sci. Technol. 2011;45:7408-7415.   DOI
51 Wang X, Hu M, Xia Y, Wen X, Ding K. Pyrosequencing analysis of bacterial diversity in 14 wastewater treatment systems in china. Appl. Environ. Microbiol. 2012;78:7042-7047.   DOI