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http://dx.doi.org/10.4491/eer.2016.005

Anaerobic digestate as a nutrient medium for the growth of the green microalga Neochloris oleoabundans  

Abu Hajar, Husam A. (Department of Civil Engineering, Ohio University)
Guy Riefler, R. (Department of Civil Engineering, Ohio University)
Stuart, Ben J. (Department of Civil & Environmental Engineering, Old Dominion University)
Publication Information
Environmental Engineering Research / v.21, no.3, 2016 , pp. 265-275 More about this Journal
Abstract
In this study, the microalga Neochloris oleoabundans was cultivated in a sustainable manner using diluted anaerobic digestate to produce biomass as a potential biofuel feedstock. Prior to microalgae cultivation, the anaerobic digestate was characterized and several pretreatment methods including hydrogen peroxide treatment, filtration, and supernatant extraction were investigated and their impact on the removal of suspended solids as well as other organic and inorganic matter was evaluated. It was found that the supernatant extraction was the most convenient pretreatment method and was used afterwards to prepare the nutrient media for microalgae cultivation. A bench-scale experiment was conducted using multiple dilutions of the supernatant and filtered anaerobic digestate in 16 mm round glass vials. The results indicated that the highest growth of the microalga N. oleoabundans was achieved with a total nitrogen concentration of 100 mg N/L in the 2.29% diluted supernatant in comparison to the filtered digestate and other dilutions.
Keywords
Anaerobic digestion; Biofuels; Microalgae; Neochloris oleoabundans;
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1 Gouveia L, Marques A, Da Silva T, Reis A. Neochloris oleoabundans UTEX #1185: A suitable renewable lipid source for biofuel production. J. Ind. Microbiol. Biotechnol. 2009;36:821-826.   DOI
2 Gouveia L, Oliveira A. Microalgae as a raw material for biofuels production. J. Ind. Microbiol. Biotechnol. 2009;36:269-274.   DOI
3 Levine RB, Costanza-Robinson MS, Spatafora GA. Neochloris oleoabundans grown on anaerobically digested dairy manure for concomitant nutrient removal and biodiesel feedstock production. Biomass Bioenerg. 2009;35:40-49.
4 Pruvost J, Van Vooren G, Cogne G, Legrand J. Investigation of biomass and lipids production with Neochloris oleoabundans in photobioreactor. Bioresour. Technol. 2009;100:5988-5995.   DOI
5 Gour RS, Kant A, Chauhan RS. Screening of micro algae for growth and lipid accumulation properties. J. Algal. Biomass Utln. 2014;5:38-46.
6 Li Y, Horsman M, Wang B, Wu N, Lan C. Effects of nitrogen sources on cell growth and lipid accumulation of green alga Neochloris oleoabundans. Appl. Microbiol. Biotechnol. 2008;81:629-636.   DOI
7 Meti BS, Sailaja B. Treatment of sugar process waste water and biogas production using algal biomass. Int. J. Eng. Res. Technol. 2014;3:61-67.
8 James SC, Boriah V. Modeling algae growth in an open-channel raceway. J. Comput. Biol. 2010;17:895-906.   DOI
9 Terry KT, Raymond LP. System design for the autotrophic production of microalgae. Enzyme Microb. Technol. 1985;7:474-487.   DOI
10 Santos AM, Lamers PP, Janssen M, Wijffels RH. Biomass and lipid productivity of Neochloris oleoabundans under alkaline-saline conditions. Algal Res. 2013;2:204-211.   DOI
11 Da Silva TL, Reis A, Medeiros R, Oliveira AC, Gouveia L. Oil production towards biofuel from autotrophic microalgae semicontinuous cultivations monitorized by flow cytometry. Appl. Biochem. Biotech. 2009;159:568-578.   DOI
12 Giovanardi M, Baldisserotto C, Ferroni L, Longoni P, Cella R, Pancaldi S. Growth and lipid synthesis promotion in mixotrophic Neochloris oleoabundans (Chlorophyta) cultivated with glucose. Protoplasma 2014;251:115-125.   DOI
13 Urreta I, Ikaran Z, Janices I, et al. Revalorization of Neochloris oleoabundans biomass as source of biodiesel by concurrent production of lipids and carotenoids. Algal Res. 2014;5:16-22.   DOI
14 Wang B, Lan CQ. Biomass production and nitrogen and phosphorus removal by the green alga Neochloris oleoabundans in simulated wastewater and secondary municipal wastewater effluent. Bioresour. Technol. 2011;102:639-644.
15 Yang Y, Mininberg B, Tarbet A, Weathers P. At high temperature lipid production in Ettlia oleoabundans occurs before nitrate depletion. Appl. Microbiol. Biot. 2013;97:2263-2273.   DOI
16 Choi HJ, Lee SM. Effects of microalgae on the removal of nutrients from wastewater: Various concentrations of Chlorella vulgaris. Environ. Eng. Res. 2012;17:S3-S8.   DOI
17 Tam NFY, Wong YS. The comparison of growth and nutrient removal efficiency of Chlorella pyrenoidosa in settled and activated sewages. Environ. Pollut. 1990;65:93-108.   DOI
18 Park K, Lim B, Lee K. Growth of microalgae in diluted process water of the animal wastewater treatment plant. Water Sci. Technol. 2009;59:2111-2116.   DOI
19 Tam NFY, Wong YS. Effect of ammonia concentrations on growth of Chlorella vulgaris and nitrogen removal from media. Bioresour. Technol. 1996;57:45-50.   DOI
20 Park J, Jin HF, Lim BR, Park KY, Lee K. Ammonia removal from anaerobic digestion effluent of livestock waste using green alga Scenedesmus sp. Bioresour. Technol. 2010;101:8649-8657.   DOI
21 Franchino M, Comino E, Bona F, Riggio VA. Growth of three microalgae strains and nutrient removal from an agro-zootechnical digestate. Chemosphere 2013;92:738-744.   DOI
22 Yang Y, Xu J, Vail D, Weathers P. Ettlia oleoabundans growth and oil production on agricultural anaerobic waste effluents. Bioresour. Technol. 2011;102:5076-5082.   DOI
23 Sturm BS, Lamer SL. An energy evaluation of coupling nutrient removal from wastewater with algal biomass production. Appl. Energ. 2011;88:3499-3506.   DOI
24 Clesceri L, Greenberg A, Eaton A. Standard Methods for the Examination of Water and Wastewater, 20th ed. Washington D.C: American Public Health Association; 1998.
25 Crittenden JC, Trussell RR, Hand DW, Howe KJ, Tchobanoglous G. Water treatment principles and design. Hoboken, NJ: John Wiley & Sons; 2005.
26 Ksibi M. Chemical oxidation with hydrogen peroxide for domestic wastewater treatment. Chem. Eng. J. 2006;119:161-165.   DOI
27 James DE. Culturing algae. Carolina Biological Supply Company; 1978.
28 Forster CF. The surface of activated sludge particles in relation to their settling characteristics. Water Res. 1968;2:767-776.   DOI
29 Pere J, Alen R, Viikari L, Eriksson L. Characterization and dewatering of activated sludge from the pulp and paper industry. Water Sci. Technol. 1993;28:193-201.
30 Liao BQ, Allen DG, Droppo IG, Leppard GG, Liss SN. Surface properties of sludge and their role in bioflocculation and settleability. Water Res. 2001;35:339-350.   DOI
31 Travieso L, Benitez F, Sanchez E, Borja R, Martin A, Colmenarejo MF. Batch mixed culture of Chlorella vulgaris using settled and diluted piggery waste. Ecol. Eng. 2006;28:158-165.   DOI
32 Su B, Qu Z, Song Y, Jia L, Zhu J. Investigation of measurement methods and characterization of zeta potential for aerobic granular sludge. J. Environ. Chem. Eng. 2014;2:1142-1147.   DOI
33 Morgan JW, Forster CF, Evison L. A comparative study of the nature of biopolymers extracted from anaerobic and activated sludges. Water Res. 1990;24:743-750.   DOI
34 Roudsari FP, Mehrnia MR, Asadi A, Moayedi Z, Ranjbar R. Effect of microalgae/activated sludge ratio on cooperative treatment of anaerobic effluent of municipal wastewater. Appl. Biochem. Biotech. 2014;172:131-140.   DOI
35 Carlsson B. An introduction to sedimentation theory in wastewater treatment. Systems and Control Group, Uppsala University; 1998.
36 Mata TM, Martins AA, Caetano NS. Microalgae for biodiesel production and other applications: A review. Renew. Sust. Energ. Rev. 2010;14:217-232.   DOI
37 Menetrez MY. An overview of algae biofuel production and potential environmental impact. Environ. Sci. Technol. 2012;46:7073-7085.   DOI
38 Rodolfi L, Zittelli GC, Bassi N, et al. Microalgae for oil: Strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor. Biotechnol. Bioeng. 2009;102:100-112.   DOI
39 Sheehan J, Dunahay T, Benemann J, Roessler P. A look back at the U.S. Department of Energy's Aquatic Species Program-Biodiesel from algae. U.S. Report NREL/TP-580-24190. National Renewable Energy Laboratory: Golden CO; 1998.