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http://dx.doi.org/10.1016/j.jiec.2018.03.028

Dual-functionalized calcium nanocomplexes for transfection of cancerous and stem cells: Low molecular weight polycation-mediated colloidal stability and ATP-mediated endosomal release  

Choi, Yeon Su (Department of Pharmacy, Integrated Research Institute of Pharmaceutical Sciences, and BK21 PLUS Team for Creative Leader Program for Pharmacomics-based Future Pharmacy, College of Pharmacy, The Catholic University of Korea)
Kim, Kyoungnam (Department of Pharmacy, Integrated Research Institute of Pharmaceutical Sciences, and BK21 PLUS Team for Creative Leader Program for Pharmacomics-based Future Pharmacy, College of Pharmacy, The Catholic University of Korea)
Ryu, Kitae (Department of Pharmacy, Integrated Research Institute of Pharmaceutical Sciences, and BK21 PLUS Team for Creative Leader Program for Pharmacomics-based Future Pharmacy, College of Pharmacy, The Catholic University of Korea)
Cho, Hana (Department of Pharmacy, Integrated Research Institute of Pharmaceutical Sciences, and BK21 PLUS Team for Creative Leader Program for Pharmacomics-based Future Pharmacy, College of Pharmacy, The Catholic University of Korea)
Cho, Yong-Yeon (Department of Pharmacy, Integrated Research Institute of Pharmaceutical Sciences, and BK21 PLUS Team for Creative Leader Program for Pharmacomics-based Future Pharmacy, College of Pharmacy, The Catholic University of Korea)
Lee, Joo Young (Department of Pharmacy, Integrated Research Institute of Pharmaceutical Sciences, and BK21 PLUS Team for Creative Leader Program for Pharmacomics-based Future Pharmacy, College of Pharmacy, The Catholic University of Korea)
Lee, Hye Suk (Department of Pharmacy, Integrated Research Institute of Pharmaceutical Sciences, and BK21 PLUS Team for Creative Leader Program for Pharmacomics-based Future Pharmacy, College of Pharmacy, The Catholic University of Korea)
Kim, Byung Gon (Department of Brain Science and Department of Neurology, School of Medicine, Ajou University)
Song, Soo Chang (Center for Biomaterials, Korea Institute of Science and Technology (KIST))
Kang, Han Chang (Department of Pharmacy, Integrated Research Institute of Pharmaceutical Sciences, and BK21 PLUS Team for Creative Leader Program for Pharmacomics-based Future Pharmacy, College of Pharmacy, The Catholic University of Korea)
Publication Information
Journal of Industrial and Engineering Chemistry / v.64, no., 2018 , pp. 300-310 More about this Journal
Abstract
To overcome colloidal instability of calcium phosphate nanoparticles in gene delivery, colloidally stable and endosomolytic $Ca^{2+}-based$ pDNA nanocomplexes (NCs) were designed by a surface coating of biocompatible polycations (PCs; low molecular weight branched polyethyleneimine [bPEI], protamine sulfate and ${\varepsilon}-polylysine$) and the addition of natural and endosomolytic ATP, respectively. Without remarkable cytotoxicity and colloidal instability, $Ca^{2+}/ATP-pDNA/bPEI_{1.8kDa}$ NCs having $[bPEI_{1.8kDa}]=3.6{\mu}g$ showed 5.8-fold and 4.4-fold higher transfection efficiencies than $bPEI_{25kDa}/pDNA$ NCs in HepG2 cells and dental pulp stem cells, respectively. In conclusion, pH-sensitive endosomolytic ATP and $Ca^{2+}-based$ gene complexes could be potentials as effective and safe gene delivery vectors in various cells.
Keywords
Adenosine triphosphate; Polycation; Calcium ions; Endosomal escape; pH-responsive;
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