The electrochemical enzymatic glucose biosensor based on mesoporous carbon fibers activated by potassium carbonate

Glucose-sensing electrodes have fabricated using mesoporous electro-spun carbon fibers, which have mesopores mainly and more oxygen functional groups on the surface, activated by potassium carbonate ($K_2CO_3$) with different concentrations. These porosity and the functional groups on the carbon fibers affected the immobilization of glucose oxidase on the electrode. The sensitivity of the electrode is determined based on enzymatic activity increasing glucose concentrations and calculated by enzyme kinetics using the Michaelis-Menten equation. The electrode prepared from carbon fibers activated with $4M\;K_2CO_3$, which has the highest mesopore volume and specific surface area, has the sensitivity $3.4{\mu}A\;mM^{-1}\;cm^{-2}$ and the broad linear range of up to 20 mM.