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http://dx.doi.org/10.3746/pnf.2012.17.2.122

Inhibition of Reactive Oxygen Species (ROS) and Nitric Oxide (NO) by Gelidium elegans Using Alternative Drying and Extraction Conditions in 3T3-L1 and RAW 264.7 Cells  

Jeon, Hui-Jeon (Department of Biomedical Science, CHA University)
Choi, Hyeon-Son (Department of Food Science and Biotechnology, CHA University)
Lee, OK-Hwan (Department of Food Science and Biotechnology, Kangwon National University)
Jeon, You-Jin (Department of Marine Life Science, Jeju National University)
Lee, Boo-Yong (Department of Food Science and Biotechnology, CHA University)
Publication Information
Preventive Nutrition and Food Science / v.17, no.2, 2012 , pp. 122-128 More about this Journal
Abstract
Gelidium (G.) elegans is a red alga inhabiting intertidal areas of North East Asia. We examined anti-oxidative and anti-inflammatory effects of G. elegans, depending on drying and extraction conditions, by determining reactive oxygen species (ROS) and nitric oxide (NO) in 3T3-L1 and RAW 264.7 cells. Extraction yields of samples using hot air drying (HD) and far-infrared ray drying (FID) were significantly higher than those using natural air drying (ND). The 70% ethanol extracts showed the highest total phenol and flavonoid contents compared to other extracts (0, 30, and 50% ethanol) under tested drying conditions. The scavenging activity on 2,2-diphenyl- 1-picrylhydrazyl (DPPH) and nitrite correlated with total phenol or flavonoid content in the extracts. The greatest DPPH scavenging effect was observed in 70% ethanol extract from FID and HD conditions. The production of ROS and NO in 3T3-L1 and macrophage cells greatly decreased with the 70% ethanol extraction derived from FID. This study suggests that 70% ethanol extraction of G. elegans dried by FID is the most optimal condition to obtain efficiently antioxidant compounds of G. elegans.
Keywords
Gelidium elegans; radical scavenging activity; ROS/RNS production; far-infrared ray drying; ethanol extraction;
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