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http://dx.doi.org/10.3746/jfn.2009.14.3.260

Determination of Tocopherol Contents in Refined Edible Oils Using an HPLC Method  

Hu, Jiang-Ning (Department of Food Science and Technology, Chungnam National University)
Zhu, Xue-Mei (Department of Food Science and Technology, Chungnam National University)
Adhikari, Prakash (Department of Food Science and Technology, Chungnam National University)
Li, Dan (Department of Food Science and Technology, Chungnam National University)
Kim, In-Hwan (Department of Food and Nutrition, Korea University)
Lee, Ki-Teak (Department of Food Science and Technology, Chungnam National University)
Publication Information
Preventive Nutrition and Food Science / v.14, no.3, 2009 , pp. 260-264 More about this Journal
Abstract
A high-performance liquid chromatography method was applied to determine the contents of tocopherols in edible oils using a LiChrosorb DIOL HPLC column and hexane fortified with 0.1% acetic acid in an isocratic mode. The validation of the method included tests for linearity, sensitivity, accuracy, precision, and recovery. All calibration curves showed good linear regression ($r^2$>0.9995) within the tested ranges. The established method offered good precision and accuracy with overall intra-day and inter-day variations of 0.94$\sim$4.27 and 1.77$\sim$ 4.88%, respectively. The tocopherol recoveries ranged from 91.44$\sim$108.90%. Subsequently, the method was successfully applied to qualitatively and quantitatively determine the total contents of $\alpha$, $\gamma$, and $\delta$-tocopherols in 12 selected refined edible oils, showing a range of 0.92 to 188.71 mg/100 g.
Keywords
refined edible oils; tocopherols; HPLC separation;
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Times Cited By KSCI : 1  (Citation Analysis)
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