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http://dx.doi.org/10.3746/jfn.2008.13.2.090

Protective Effect of Chungkukjang from Sunchang Province against Cellular Oxidative Damage  

Choi, Ji-Myung (Department of Food Science and Nutrition, and Research Institute of Ecology for the Elderly, Pusan National University)
Yi, Na-Ri (Department of Food Science and Nutrition, and Research Institute of Ecology for the Elderly, Pusan National University)
Seo, Kyoung-Chun (Department of Food Science and Nutrition, and Research Institute of Ecology for the Elderly, Pusan National University)
Han, Ji-Sook (Department of Food Science and Nutrition, and Research Institute of Ecology for the Elderly, Pusan National University)
Song, Young-Ok (Department of Food Science and Nutrition, and Research Institute of Ecology for the Elderly, Pusan National University)
Cho, Eun-Ju (Department of Food Science and Nutrition, and Research Institute of Ecology for the Elderly, Pusan National University)
Publication Information
Preventive Nutrition and Food Science / v.13, no.2, 2008 , pp. 90-94 More about this Journal
Abstract
The protective effect of chungkukjang from Sunchang province against oxidative stress was evaluated in the cellular system using LLC-$PK_1$ renal epithelial cells. The LLC-$PK_1$ cells showed decrease in cell viability and elevation in lipid peroxidation by the treatment with the generators of nitric oxide (NO) and superoxide anion ($O_2^-$) produced by sodium nitrouprusside and pyrogallol, respectively. However, the methanol extract of chungkukjang significantly inhibited cellular loss and lipid peroxidation in a dose-dependent manner; in particular K chungkukjang (KC) exerted the strongest protective effect. In addition, the protective effect of chungkukjang from 3-morpholinosydnonimine, as a source of peroxynitrite, with simultaneous generations of NO and $O_2^-$, was also studied. Treatment with chungkukjangs significantly preserved the cell viability and inhibited lipid peroxidation caused by SIN-1 with dose-dependence. The present study suggests that chungkukjang from Sunchang province, especially KC, would have protective potential from oxidative stress induced by free radicals under cellular oxidative damage.
Keywords
chungkukjang; LLC-$PK_1$; nitric oxide; superoxide anion; peroxynitrite;
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Times Cited By KSCI : 6  (Citation Analysis)
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