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Electrochemical Gene Detection Using Microelectrode Array on a DNA Chip  

Park, Yong-Sung (School of Electrical, Electronic and Information Engineering, Wonkwang University)
Kwon, Young-Soo (Div. of Electrical, Electronic and Computer Eng., Dong-A University)
Park, Dae-Hee (School of Electrical, Electronic and Information Engineering, Wonkwang University)
Publication Information
KIEE International Transactions on Electrophysics and Applications / v.4C, no.4, 2004 , pp. 145-148 More about this Journal
Abstract
In this study, a DNA chip with a microelectrode array was fabricated using microfabrication technology. Several probe DNAs consisting of mercaptohexyl moiety at their 5' end were immobilized on the gold electrodes by a DNA arrayer. Then target DNAs were hybridized and reacted with Hoechst 33258, which is a DNA minor groove binder and electrochemically active dye. Linear sweep voltammetry or cyclic voltammetry showed a difference between target DNA and control DNA in the anodic peak current values. It was derived from Hoechst 33258 and concentrated at the electrode surface through association with the formed hybrid. This suggested that this DNA chip could recognize the sequence specific genes.
Keywords
DNA minor groove binder; Hoechst 33258; Linear sweep voltammetry Mercaptohexyl moiety; Microfabrication technology;
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Times Cited By KSCI : 2  (Citation Analysis)
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