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http://dx.doi.org/10.5713/ajas.19.0080

Conjugated linoleic acid producing potential of lactobacilli isolated from goat (AXB) rumen fluid samples  

Tyagi, Amrish Kumar (Rumen Biotechnology Lab, Animal Nutrition Division, ICAR-National Dairy Research Institute)
Kumar, Sachin (Rumen Biotechnology Lab, Animal Nutrition Division, ICAR-National Dairy Research Institute)
Choudhury, Prasanta Kumar (Rumen Biotechnology Lab, Animal Nutrition Division, ICAR-National Dairy Research Institute)
Tyagi, Bhawna (Rumen Biotechnology Lab, Animal Nutrition Division, ICAR-National Dairy Research Institute)
Tyagi, Nitin (Rumen Biotechnology Lab, Animal Nutrition Division, ICAR-National Dairy Research Institute)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.33, no.8, 2020 , pp. 1233-1241 More about this Journal
Abstract
Objective: The present investigation was aimed to explore the potential of lactobacilli for conjugated linoleic acid (CLA) production, isolated from rumen fluid samples of lactating goats. Methods: A total of 64 isolates of lactobacilli were obtained using deMan-Rogosa-Sharpe (MRS) agar from rumen fluid of goats and further subjected to morphological and biochemical characterizations. Isolates found as gram-positive, catalase negative rods were presumptively identified as Lactobacillus species and further confirmed by genus specific polymerase chain reaction (PCR). The phylogenetic tree was constructed from the nucleotide sequences using MEGA6. Results: Out of the 64 isolates, 23 isolates were observed positive for CLA production by linoleate isomerase gene-based amplification and quantitatively by UV-spectrophotometric assay for the conversion of linoleic acid to CLA as well as gas chromatography-based assay. In all Lactobacillus species cis9, trans11 isomer was observed as the most predominant CLA isomer. These positive isolates were identified by 16S rRNA gene-based PCR sequencing and identified to be different species of L. ingluviei (2), L.salivarius (2), L. curvatus (15), and L. sakei (4). Conclusion: The findings of the present study concluded that lactic acid bacteria isolated from ruminal fluid samples of goat have the potential to produce bioactive CLA and may be applied as a direct fed microbial to enhance the nutraceutical value of animal food products.
Keywords
Biohydrogenation; Conjugated Linoleic Acid; Goat; Lactobacilli; Rumen;
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