Browse > Article
http://dx.doi.org/10.5713/ajas.19.0587

The expression and localization of V-ATPase and cytokeratin 5 during postnatal development of the pig epididymis  

Park, Yun-Jae (Department of Animal Resources Science, Kongju National University)
Kim, Ji-Hyuk (Department of Animal Resources Science, Kongju National University)
Kim, Hack-Youn (Department of Animal Resources Science, Kongju National University)
Park, Hee-Bok (Department of Animal Resources Science, Kongju National University)
Choe, Juhui (Department of Animal Resources Science, Kongju National University)
Kim, Gye-Woong (Department of Animal Resources Science, Kongju National University)
Baek, Sun-Young (Division of Swine Science, National Institute of Animal Science, Rural Development Administration)
Chung, Hak-Jae (Division of Swine Science, National Institute of Animal Science, Rural Development Administration)
Park, Yoo-Jin (Department of Animal Science and Technology, Chung-Ang University)
Kim, Bongki (Department of Animal Resources Science, Kongju National University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.33, no.7, 2020 , pp. 1077-1086 More about this Journal
Abstract
Objective: We examined the localization and expression of H+ pumping vacuolar ATPase (V-ATPase) and cytokeratin 5 (KRT5) in the epididymis of pigs, expressed in clear and basal cells, respectively, during postnatal development. Methods: Epididymides were obtained from pigs at 1, 7, 21, 60, 120, and 180 days of age; we observed the localization and expression patterns of V-ATPase and KRT5 in the different regions of these organs, namely, the caput, corpus, and cauda. The differentiation of epididymal epithelial cells was determined by immunofluorescence labeling using cell-type-specific markers and observed using confocal microscopy. Results: At postnatal day 5 (PND5), the localization of clear cells commenced migration from the cauda toward the caput. Although at PND120, goblet-shaped clear cells were detected along the entire length of the epididymis, those labeled for V-ATPase had disappeared from the corpus to cauda and were maintained only in the caput epididymis in adult pigs. In contrast, whereas basal cells labeled for KRT5 were only present in the vas deferens at birth, they were detected in all regions of the epididymis at PND60. These cells were localized at the base of the epithelium; however, no basal cells characterized by luminally extending cell projections were observed in any of the adult epididymides examined. Conclusion: The differentiation of clear and basal cells progressively initiates in a retrograde manner from the cauda to the caput epididymis. The cell-type-specific distribution and localization of the epithelial cells play important roles in establishing a unique luminal environment for sperm maturation and storage in the pig epididymis.
Keywords
Clear Cell; Basal Cell; Immunofluorescence; Epithelium; Luminal Environment;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Shum WWC, Da Silva N, Brown D, Breton S. Regulation of luminal acidification in the male reproductive tract via cell-cell crosstalk. J Exp Biol 2009;212:1753-61. https://doi.org/10.1242/jeb.027284   DOI
2 Rodriguez-Martinez H, Ekstedt E, Einarsson S. Acidification of epididymal fluid in the boar. Int J Androl 1990;13:238-43. https://doi.org/10.1111/j.1365-2605.1990.tb00982.x   DOI
3 Ramos Jr AS, Dym M. Fine structure of the monkey epididymis. Am J Anat 1977;149:501-31. https://doi.org/10.1002/aja.1001490407   DOI
4 Goyal HO. Morphology of the bovine epididymis. Am J Anat 1985;172:155-72. https://doi.org/10.1002/aja.1001720205   DOI
5 Shum WW, Ruan YC, Da Silva N, Breton S. Establishment of cell-cell cross talk in the epididymis: control of luminal acidification. J Androl 2011;32:576-86. https://doi.org/10.2164/jandrol.111.012971   DOI
6 Shum W, Hill E, Brown D, Breton S. Plasticity of basal cells during postnatal development in the rat epididymis. Reproduction 2013;146:455-69. https://doi.org/10.1530/REP-12- 0510   DOI
7 St-cyr A, Legare C, Frenette G, Gaudreault C, Sullivan R. P26h and dicarbonyl/L-xylulose reductase are two distinct proteins present in the hamster epididymis. Mol Reprod Dev 2004;69:137-45. https://doi.org/10.1002/mrd.20089   DOI
8 Frenette G, Lessard C, Madore E, Fortier MA, Sullivan R. Aldose reductase and macrophage migration inhibitory factor are associated with epididymosomes and spermatozoa in the bovine epididymis. Biol Reprod 2003;69:1586-92. https://doi.org/10.1095/biolreprod.103.019216   DOI
9 Cheung K, Leung C, Leung G, Wong P. Synergistic effects of cystic fibrosis transmembrane conductance regulator and aquaporin-9 in the rat epididymis. Biol Reprod 2003;68:1505-10. https://doi.org/10.1095/biolreprod.102.010017   DOI
10 Kirchhoff C, Krull N, Pera I, Ivell R. A major mRNA of the human epididymal principal cells, HE5, encodes the leucocyte differentiation CDw52 antigen peptide backbone. Mol Reprod Dev 1993;34:8-15. https://doi.org/10.1002/mrd.1080340103   DOI
11 Shum WW, Da Silva N, Brown D, Breton S. Regulation of luminal acidification in the male reproductive tract via cell-cell crosstalk. J Exp Biol 2009;212:1753-61. https://doi.org/10.1242/jeb.027284   DOI
12 Harvey WR, Xiang MA. K+ pump: from caterpillar midgut to human cochlea. J Insect Physiol 2012;58:590-8. https://doi.org/10.1016/j.jinsphys.2012.03.001   DOI
13 Karet FE. Physiological and metabolic implications of V-ATPase isoforms in the kidney. J Bioenerg Biomembr 2005;37:425-9. https://doi.org/10.1007/s10863-005-9484-x   DOI
14 Shum WW, Smith TB, Cortez-Retamozo V, et al. Epithelial basal cells are distinct from dendritic cells and macrophages in the mouse epididymis. Biol Reprod 2014;90:90. https://doi.org/10.1095/biolreprod.113.116681   DOI
15 Miller RL, Zhang P, Smith M, et al. V-ATPase B1-subunit promoter drives expression of EGFP in intercalated cells of kidney, clear cells of epididymis and airway cells of lung in transgenic mice. Am J Physiol Cell Physiol 2005;288:C1134-44. https://doi.org/10.1152/ajpcell.00084.2004   DOI
16 Hermo L, Dworkin J, Oko R. Role of epithelial clear cells of the rat epididymis in the disposal of the contents of cytoplasmic droplets detached from spermatozoa. Am J Anat 1988; 183:107-24. https://doi.org/10.1002/aja.1001830202   DOI
17 Robinson EJ, Neal DE, Collins AT. Basal cells are progenitors of luminal cells in primary cultures of differentiating human prostatic epithelium. Prostate 1998;37:149-60. https://doi.org/10.1002/(SICI)1097-0045(19981101)37:3<149::AID-PROS4>3.0.CO;2-E   DOI
18 Rock JR, Onaitis MW, Rawlins EL, et al. Basal cells as stem cells of the mouse trachea and human airway epithelium. Proc Natl Acad Sci USA 2009;106:12771-5. https://doi.org/10.1073/pnas.0906850106   DOI
19 Shum WW, Da Silva N, McKee M, Smith PJ, Brown D, Breton S. Transepithelial projections from basal cells are luminal sensors in pseudostratified epithelia. Cell 2008;135:1108-17. https://doi.org/10.1016/j.cell.2008.10.020   DOI
20 Park YJ, Battistone MA, Kim B, Breton S. Relative contribution of clear cells and principal cells to luminal pH in the mouse epididymis. Biol Reprod 2017;96:937. https://doi.org/10.1093/biolre/iox011   DOI
21 Sun EL, Flickinger CJ. Development of cell types and of regional differences in the postnatal rat epididymis. Am J Anat 1979;154:27-55. https://doi.org/10.1002/aja.1001540104   DOI
22 Schimming B, Baumam C, Pinheiro P, de Matteis R, Domeniconi R. Aquaporin 9 is expressed in the epididymis of immature and mature pigs. Reprod Domest Anim 2017;52:617-24. https://doi.org/10.1111/rda.12957   DOI
23 Breton S, Tyszkowski R, Sabolic I, Brown D. Postnatal development of H+ ATPase (proton-pump)-rich cells in rat epididymis. Histochem Cell Biol 1999;111:97-105. https://doi.org/10.1007/s004180050339   DOI
24 Da Silva N, Silberstein C, Beaulieu V, et al. Postnatal expression of aquaporins in epithelial cells of the rat epididymis. Biol Reprod 2006;74:427-38. https://doi.org/10.1095/biolreprod. 105.044735   DOI
25 Lee W-Y, Lee K-H, Heo Y-T, et al. Transcriptional coactivator undifferentiated embryonic cell transcription factor 1 expressed in spermatogonial stem cells: a putative marker of boar spermatogonia. Anim Reprod Sci 2014;150:115-24. https://doi.org/10.1016/j.anireprosci.2014.09.010   DOI
26 Kim B, Roy J, Shum WW, Da Silva N, Breton S. Role of testicular luminal factors on Basal cell elongation and proliferation in the mouse epididymis. Biol Reprod 2015;92:9. https://doi.org/10.1095/biolreprod.114.123943   DOI
27 Castro MM, Kim B, Hill E, et al. The expression patterns of aquaporin 9, vacuolar H(+)-ATPase, and cytokeratin 5 in the epididymis of the common vampire bat. Histochem Cell Biol 2017;147:39-48. https://doi.org/10.1007/s00418-016-1477-9   DOI
28 Pastor-Soler N, Isnard-Bagnis C, Herak-Kramberger C, et al. Expression of aquaporin 9 in the adult rat epididymal epithelium is modulated by androgens. Biol Reprod 2002;66:1716-22. https://doi.org/10.1095/biolreprod66.6.1716   DOI
29 Hamzeh M, Robaire B. Effect of testosterone on epithelial cell proliferation in the regressed rat epididymis. J Androl 2009;30:200-12. https://doi.org/10.2164/jandrol.108.006171   DOI
30 Jun HJ, Roy J, Smith TB, et al. ROS1 signaling regulates epithelial differentiation in the epididymis. Endocrinology 2014;155:3661-73. https://doi.org/10.1210/en.2014-1341   DOI
31 Hermo L, Adamali HI, Andonian S. Immunolocalization of CA II and H+ V-ATPase in epithelial cells of the mouse and rat epididymis. J Androl 2000;21:376-91. https://doi.org/10.1002/j.1939-4640.2000.tb03392.x