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http://dx.doi.org/10.5713/ajas.19.0295

Effects of Astragalus membranaceus roots supplementation on growth performance, serum antioxidant and immune response in finishing lambs  

Hao, Xiaoyan (College of Animal Science and Veterinary, Shanxi Agricultural University)
Wang, Pengju (College of Animal Science and Veterinary, Shanxi Agricultural University)
Ren, Youshe (College of Animal Science and Veterinary, Shanxi Agricultural University)
Liu, Gentang (Institution of Husbandry and Veterinary)
Zhang, Jianxin (College of Animal Science and Veterinary, Shanxi Agricultural University)
Leury, Brian (Faculty of Veterinary and Agricultural Sciences, University of Melbourne)
Zhang, Chunxiang (College of Animal Science and Veterinary, Shanxi Agricultural University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.33, no.6, 2020 , pp. 965-972 More about this Journal
Abstract
Objective: Astragalus membranaceus root is a well-known traditional Chinese herbal medicine with many biological active constituents. This study was conducted to examine the effects of Astragalus membranaceus root powder (AMP) on growth performance, serum antioxidant and immune response in finishing lambs. Methods: A total of thirty-six Guangling fat-tailed ram lambs (body weight = 19±2 kg, mean±standard deviation) were randomly assigned to one of six treatments for a 40 d feeding period, with the first 10 d for adaptation. Treatments consisted of the lambs' basal diets with addition of 0, 5, 10, 15, 20, and 30 g/kg of diet of AMP. Results: Response to supplementation level of AMP was quadratic (p≤0.032) for final weight and ADG with the greatest at 10 g/kg of diet, but dry matter intake was not affected (p≥0.227) by treatments. The increase of AMP supplementation resulted in a quadratic response in contents of triglyceride and creatinine (p<0.05), with the lowest values for 10 and 20 g/kg of diet, respectively. A linear and quadratic decrease was observed in activity of alkaline phosphatase in serum of lambs. As the AMP supplementation increased, the activities of total superoxide dismutase and total antioxidant capacity increased linearly (p≤0.018) and hydroxyl radical (OH-) decreased linearly (p = 0.002). For catalase (CAT) and malondialdehyde (MDA), quadratic (p≤0.001) effects were observed among treatments, with the greatest CAT and lowest MDA values at 10 g/kg AMP. Additionally, supplementing AMP up to a level of 10 or 15 g/kg of diet quadratically increased immunoglobulin and interleukin contents in the serum. Conclusion: The results indicated that AMP can be used as natural feed additive in the ration of lambs to improve ADG, antioxidant status, and immune functions, and the optimal dose was 10 g/kg of diet under the condition of this experiment.
Keywords
Astragalus membranaceus Root; Growth Performance; Antioxidant Status; Immune Response; Lamb;
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1 Peng HS, Wang J, Zhang HT, et al. Rapid identification of growth years and profiling of bioactive ingredients in Astragalus membranaceus var. mongholicus (Huangqi) roots from Hunyuan, Shanxi. Chin Med 2017;12:14. https://doi.org/10.1186/s13020-017-0135-z   DOI
2 Liu W, Gao FF, Li Q, et al. Protective effect of Astragalus polysaccharides on liver injury induced by several different chemotherapeutics in mice. Asian Pac J Cancer Prev 2014;15: 10413-20. https://doi.org/10.7314/APJCP.2014.15.23.10413   DOI
3 Ding YY, Zhang CH, He XL, Huang L, Yin ZJ. Growth performance responses and indicators of gastrointestinal health in early weaned pigs fed Chinese herbal medicine additives-supplemented diets. J Anim Vet Adv 2011;12:1580-7. https://doi.org/10.3923/javaa.2011.1580.1587
4 Zuo ZY, Yang WR, Wang Y, Yang ZB, Jiang SZ, Zhang GG. Effects of Astragalus membranaceus on laying performance and antioxidant status of laying hens. J Appl Poult Res 2012; 21:243-50. https://doi.org/10.3382/japr.2011-00351   DOI
5 Nie X, Shi B, Ao Z, Yin G, Tao W. Extraction of the Astragalus polysaccharides and its effects on the lymphocytes proliferation. J Anhui Agric Univ 2004;31:34-6.   DOI
6 Rui L, Chen WC, Wang WP, Tian WY, Zhang XG. Antioxidant activity of Astragalus polysaccharides and antitumour activity of the polysaccharides and siRNA. Carbohydr Polym 2010;82:240-4. https://doi.org/10.1016/j.carbpol.2010.02.048   DOI
7 Wang HF, Yang WR, Yang HW, et al. Effects of Astragalus membranaceus on growth performance, carcass characteristics, and antioxidant status of broiler chickens. Acta Agric Scand 2010;60:151-8. https://doi.org/10.1080/09064702.2010. 511255
8 Yang L, Wang ZH, Tao JS. Comparison of the methods for determination of Astraglus polysaccharides in radix Astragali. Chin J Pharm 2005;36:562-3. https://doi.org/10.3969/j.issn.1001-8255.2005.09.019   DOI
9 Nutrient requirements of small ruminants, sheep, goats, cervids and New World Camelids. Washington, DC, USA: National Academy Press; 2007.
10 Association of Official Analytical Chemists (AOAC). Official Methods of Analysis. 15th ed. Arlington, VA, USA: Association of Official Analytical Chemists; 1990.
11 Van Soest PJ, Robertson JB, Lewis BA. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci 1991;74:3583-97. https://doi.org/10.3168/jds.S0022-0302(91)78551-2   DOI
12 Jin M, Zhao K, Huang Q, Peng S. Structural features and biological activities of the polysaccharides from Astragalus membranaceus. Int J Biol Macromol 2014;64:257-66. https://doi.org/10.1016/j.ijbiomac.2013.12.002   DOI
13 Gee JM, Wal JM, Miller K, et al. Effect of saponin on the transmucosal passage of $\beta$-lactoglobulin across the proximal small intestine of normal and $\beta$-lactoglobulin-sensitised rats. Toxicology 1997;117:219-28. https://doi.org/10.1016/S0300-483X(96)03574-3   DOI
14 Zhong RZ, Yu M, Liu HW, Sun HX, Cao Y, Zhou DW. Effects of dietary Astragalus polysaccharide and Astragalus membranaceus root supplementation on growth performance, rumen fermentation, immune responses, and antioxidant status of lambs. Anim Feed Sci Technol 2012;174:60-7. https://doi.org/10.1016/j.anifeedsci.2012.02.013   DOI
15 Ma XQ, Shi Q, Duan JA, Dong TTX, Tsim KWK. Chemical analysis of Radix Astragali (Huangqi) in China: a comparison with its adulterants and seasonal variations. J Agric Food Chem 2002;50:4861-6. https://doi.org/10.1021/jf0202279   DOI
16 Li L, Zheng S, Yang Q, Chen S, Huang L. Distinguishing Astragalus mongholicus and its planting soil samples from different regions by ICP-AES. Molecules 2016;21:482. https://doi.org/10.3390/molecules21040482   DOI
17 Chen RZ, Tan L, Jin CG, et al. Extraction, isolation, characterization and antioxidant activity of polysaccharides from Astragalus membranaceus. Ind Crops Prod 2015;77:434-43. https://doi.org/10.1016/j.indcrop. 2015.08.006   DOI
18 Jenkins KJ, Atwal AS. Effects of dietary saponins on fecal bile acids and neutral sterols, and availability of vitamins A and E in the chick. J Nutr Biochem 1994;5:134-7. https://doi.org/ 10.1016/0955-2863(94)90084-1   DOI
19 Zhang GG, Yang ZB, Wang Y, Yang WR. Effects of Astragalus membranaceus root processed to different particle sizes on growth performance, antioxidant status, and serum metabolites of broiler chickens. Poult Sci 2013;92:178-83. https://doi.org/10.3382/ps.2012-02432   DOI
20 Mao XF, Piao XS, Lai CH, Li DF, Xing JJ, Shi BL. Effects of beta-glucan obtained from the Chinese herb Astragalus membranaceus and lipopolysaccharide challenge on performance, immunological, adrenal, and somatotropic responses of weanling pigs. J Anim Sci 2005;83:2775-82. https://doi.org/10.2527/2005.83122775x   DOI
21 Zhang YW, Wu CY, Cheng JT. Merit of Astragalus polysaccharide in the improvement of early diabetic nephropathy with an effect on mRNA expressions of NF-${\kappa}B$ and $I{\kappa}B$ in renal cortex of streptozotoxin-induced diabetic rats. J Ethnopharmacol 2007;114:387-92. https://doi.org/10.1016/j.jep.2007. 08.024   DOI
22 Janibekova AA, Youssefb FS, Ashourb ML, Mamadalievaa NZ. New flavonoid glycosides from two Astragalus species (Fabaceae) and validation of their antihyperglycaemic activity using molecular modelling and in vitro studies. Ind Crops Prod 2018;118:142-8. https://doi.org/10.1016/j.indcrop.2018. 03.034   DOI
23 Zou F, Mao XQ, Wang N, Liu J, Ou-Yang JP. Astragalus polysaccharides alleviates glucose toxicity and restores glucose homeostasis in diabetic states via activation of AMPK. Acta Pharmacol Sin 2009;30:1607-15. https://doi.org/10.1038/aps.2009.168   DOI
24 Chen W, Li YM, Yu MH. Astragalus polysaccharides inhibited diabetic cardiomyopathy in hamsters depending on suppression of heart chymase activation. J Diabetes Complications 2010;24:199-208. https://doi.org/10.1016/j.jdiacomp.2008. 12.003   DOI
25 Anoopraj R, Hemalatha S, Balachandran C. A preliminary study on serum liver function indices of diethylnitrosamine induced hepatocarcinogenesis and chemoprotective potential of Eclipta alba in male Wistar rats. Vet World 2014;7:439-42. https://doi.org/10.14202/vetworld.2014.439-442   DOI
26 Waikar SS, Betensky RA, Bonventre JV. Creatinine as the gold standard for kidney injury biomarker studies? Nephrol Dial Transplant 2009;24:3263-5. https://doi.org/10.1093/ndt/gfp428   DOI
27 Sun WY, Wei W, Wu L, Gui SY, Wang H. Effects and mechanisms of extract from Paeonia lactiflora and Astragalus membranaceus on liver fibrosis induced by carbon tetrachloride in rats. J Ethnopharmacol 2007;112:514-23. https://doi.org/10.1016/j.jep.2007.04.005   DOI
28 Miyazaki Y, Yamasaki M, Mishima H, Mansho K, Tachibana H, Yamada K. Oxidative stress by visible light irradiation suppresses immunoglobulin production in mouse spleen lymphocytes. Biosci Biotechnol Biochem 2001;65:593-8. https://doi.org/10.1271/bbb.65.593   DOI
29 Kim JS, Yean MH, Lee EJ, et al. Two new cycloartane saponins from the roots of Astragalus membranaceus. Chem Pharm Bull 2008;56:105-8. https://doi.org/10.1248/cpb.56.105   DOI
30 Yan H, Xie YP, Sun SG, et al. Chemical analysis of Astragalus mongholicus polysaccharides and antioxidant activity of the polysaccharides. Carbohydr Polym 2010;82:636-40. https://doi.org/10.1016/j.carbpol.2010.05.026   DOI
31 Jiang J, Wu C, Gao H, Song J, Li H. Effects of astragalus polysaccharides on immunologic function of erythrocyte in chickens infected with infectious bursa disease virus. Vaccine 2010;28:5614-6. https://doi.org/10.1016/j.vaccine.2010.06. 025   DOI
32 Shao P, Zhao LH, Chen Z, Pan JP. Regulation on maturation and function of dendritic cells by Astragalus mongholicus polysaccharides. Int Immunopharmacol 2006;6:1161-6. https://doi.org/10.1016/j.intimp.2006.02.009   DOI
33 Qin Q, Niu J, Wang Z, Xu W, Qiao Z, Gu Y. Astragalus embranaceus extract activates immune response in macrophages via heparanase. Molecules 2012;17:7232-40. https://doi.org/10.3390/molecules17067232   DOI
34 Ma X, Tu P, Chen Y, Zhang T, Wei Y, Ito Y. Preparative isolation and purification of two isoflavones from Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao by high-speed counter-current chromatography. J Chromatogr A 2003;992:193-7. https://doi.org/10.1016/S0021-9673(03)00315-7   DOI