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http://dx.doi.org/10.5713/ajas.18.0512

Effects of feed intake on the diversity and population density of homoacetogens in the large intestine of pigs  

Matsui, Hiroki (Graduate School of Bioresources, Mie University)
Mimura, Ayumi (Graduate School of Bioresources, Mie University)
Maekawa, Sakiko (Graduate School of Bioresources, Mie University)
Ban-Tokuda, Tomomi (Graduate School of Bioresources, Mie University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.32, no.12, 2019 , pp. 1907-1913 More about this Journal
Abstract
Objective: Homoacetogens play important roles in the production of acetate in the large intestine of monogastric mammals. However, their diversity in the porcine large intestine is still unknown. Marker gene analysis was performed to assess the effects of energy level on the diversity and population densities of homoacetogens in porcine feces. Methods: Crossbred pigs were fed high or low energy-level diets. The high-intake (HI) diet was sufficient to allow a daily gain of 1.2 kg. The low-intake (LI) diet provided 0.6 times the amount of energy as the HI diet. Genetic diversity was analyzed using formyltetrahydrofolate synthetase gene (FHS) clone libraries derived from fecal DNA samples. FHS DNA copy numbers were quantified using real-time polymerase chain reaction. Results: A wide variety of FHS sequences was recovered from animals in both treatments. No differences in FHS clone libraries between the HI and LI groups were found. During the experimental period, no significant differences in the proportion of FHS copy numbers were observed between the two treatment groups. Conclusion: This is the first reported molecular diversity analysis using specific homoacetogen marker genes from the large intestines of pigs. There was no observable effect of feed intake on acetogen diversity.
Keywords
Feed Intake; Formyltetrahydrofolate Synthetase (FTHFS) Gene (FHS); Homoacetogen; Large Intestine; Pig;
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1 Engelhardt WV. Absorption of short-chain fatty acids from the large intestine. In: Cummings JH, Rombeau JL, Sakata T, editors. Physiological and clinical aspects of short-chain fatty acids. Cambridge, UK: Cambridge University Press; 1995. p. 149-70.
2 Arora T, Sharma R. Fermentation potential of the gut microbiome: implications for energy homeostasis and weight management. Nutr Rev 2011;69:99-106. https://doi.org/10.1111/j.1753-4887.2010.00365.x   DOI
3 Rose CJ, Hume ID, Farrell DJ. Fibre digestion and volatile fatty acid production in domestic and feral pigs. In: Farrell DJ, editor. Recent advances in animal nutrition in Australia. Armidale, Australia: University of New England Press; 1987. p. 347-60.
4 De Graeve KG, Grivet JP, Durand M, et al. Competition between reductive acetogenesis and methanogenesis in the pig large-intestinal flora. J Appl Bacteriol 1994;76:55-61.   DOI
5 Lajoie SF, Bank S, Miller TL, Wolin MJ. Acetate production from hydrogen and ($^{13}C$)carbon dioxide by the microflora of human feces. Appl Environ Microbiol 1988;54:2723-7.   DOI
6 Morvan B, Bonnemoy F, Fonty G, Gouet P. Quantitative determination of H2-utilizing acetogenic bacteria and sulfatereducing bacteria and methanogenic archaea from digestive tract of different mammals. Curr Microbiol 1996;32:129-33. https://doi.org/10.1007/s002849900023   DOI
7 Ohashi Y, Igarashi T, Kumazawa F, Fujisawa T. Analysis of acetogenetic bacteria in human feces with formyltetrahydrofolate synthetase sequences. Biosci Microflora 2007;26:37-40. https://doi.org/10.12938/bifidus.26.37   DOI
8 De Graeve K, Demeyer DI. Rumen and hindgut fermentation: differences for possible exploitation? Mededelingen van de Faculteit Landbouwwetenschappen Rijksuniversiteit Gent; Belgium. 1988;53:1805-9.
9 Prins RA, Lankhorst A. Synthesis of acetate from $CO_{2}$ in the cecum of some rodents. FEMS Microbiol Lett 1977;1:255-8.   DOI
10 Matsui H, Ishimoto-Tsuchiya T, Maekawa S, Ban-Tokuda T. Diversity and population density of methanogens in the large intestine of pigs fed diets of different energy levels. Anim Sci J 2018:89:1468-74. https://doi.org/10.1111/asj.13083   DOI
11 Drake HL, Kusel K, Matthies C. Ecological consequences of the phylogenetic and physiological diversities of acetogen. Antonie Van Leeuwenhoek 2002;81:203-13. https://doi.org/10.1023/A:1020514617738   DOI
12 Lovell CR, Leaphart AB. Community-level analysis: key genes of $CO_{2}$-reductive acetogenesis. Methods Enzymol 2005;397:454-69. https://doi.org/10.1016/S0076-6879(05)97028-6   DOI
13 Altschul SF, Madden TL, Schaffer AA, et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997;25:3389-402. https://doi.org/10.1093/nar/25.17.3389   DOI
14 Gagen EJ, Denman SE, Padmanabha J, et al. Functional gene analysis suggests different acetogen populations in the bovine rumen and Tammar wallaby forestomach. Appl Environ Microbiol 2010;76:7785-95. https://doi.org/10.1128/AEM.01679-10   DOI
15 Matsui H, Kojima N, Tajima K. Diversity of the formyltetrahydrofolate synthetase gene (fhs), a key enzyme for reductive acetogenesis, in the bovine rumen. Biosci Biotechnol Biochem 2008;72:3273-6. https://doi.org/10.1271/bbb.70375   DOI
16 Matsui H, Yoneda S, Ban-Tokuda T, Wakita M. Diversity of the formyltetrahydrofolate synthetase (FTHFS) gene in the proximal and mid ostrich colon. Curr Microbiol 2011;62:1-6. https://doi.org/10.1007/s00284-010-9661-y   DOI
17 National Agriculture and Bio-oriented Research Organization. Japanese feeding standard for swine. Tokyo, Japan: Japan Livestock Industry Association; 2005.
18 Hattori K, Matsui H. Diversity of fumarate reducing bacteria in the bovine rumen revealed by culture dependent and independent approaches. Anaerobe 2008;14:87-93. https://doi.org/10.1016/j.anaerobe.2007.12.002   DOI
19 Schloss PD, Handelsman J. Introducing DOTUR, a computer program for defining operational taxonomic units and estimating species richness. Appl Environ Microbiol 2005;71: 1501-6. https://doi.org/10.1128/AEM.71.3.1501-1506.2005   DOI
20 Juottonen H, Galand PE, Yrjala K. Detection of methanogenic Archaea in peat: comparison of PCR primers targeting the mcrA gene. Res Microbiol 2006;157:914-21. https://doi.org/10.1016/j.resmic.2006.08.006   DOI
21 Larkin MA, Blackshields G, Brown NP, et al. Clustal W and Clustal X version 2.0. Bioinformatics 2007;23:2947-8. https://doi.org/10.1093/bioinformatics/btm404   DOI
22 Saitou N, Nei M. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406-25. https://doi.org/10.1093/oxfordjournals.molbev.a040454
23 Guo X, Xia X, Tang R, Zhou J, Zhao H, Wang K. Development of a real-time PCR method for Firmicutes and Bacteroidetes in faeces and its application to quantify intestinal population of obese and lean pigs. Lett Appl Microbiol 2008;47:367-73. https://doi.org/10.1111/j.1472-765X.2008.02408.x   DOI
24 Singleton DR, Furlong MA, Rathbun SL, Whitman WB. Quantitative comparisons of 16S rRNA gene sequence libraries from environmental samples. Appl Environ Microbiol 2001;67:4374-6. https://doi.org/10.1128/AEM.67.9.4374-4376.2001   DOI
25 Henderson G, Naylor GE, Leahy SC, Janssen PH. Presence of novel, potentially homoacetogenic bacteria in the rumen as determined by analysis of formyltetrahydrofolate synthetase sequences from ruminants. Appl Environ Microbiol 2010;76:2058-66. https://doi.org/10.1128/AEM.02580-09   DOI
26 Leaphart AB, Lovell CR. Recovery and analysis of formyltetrahydrofolate synthetase gene sequences from natural populations of acetogenic bacteria. Appl Environ Microbiol 2001;67:1392-5. https://doi.org/10.1128/AEM.67.3.1392-1395.2001   DOI