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http://dx.doi.org/10.5713/ajas.18.0811

Partial replacement of pork backfat with konjac gel in Northeastern Thai fermented sausage (Sai Krok E-san) to produce the healthier product  

Sorapukdee, Supaluk (Department of Animal Production Technology and Fisheries, Faculty of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang)
Jansa, Sujitta (Department of Animal Production Technology and Fisheries, Faculty of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang)
Tangwatcharin, Pussadee (Department of Animal Production Technology and Fisheries, Faculty of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.32, no.11, 2019 , pp. 1763-1775 More about this Journal
Abstract
Objective: The influence of konjac gel level on fermentation process and product qualities were assessed to evaluate the feasibility of using it as fat analog in Northeastern Thai fermented sausage (Sai Krok E-san). Methods: Five treatments of fermented sausages were formulated by replacing pork backfat with 0%, 7.5%, 22.5%, and 30% konjac gel. The changes in lactic acid bacteria (LAB) and important physicochemical properties of samples were assessed during 3 days of fermentation. After the end of fermentation at day 3, water activity ($a_w$), instrumental texture, color, microbial counts, and sensory evaluation were compared. The best product formulation using konjac for replacing pork back fat were selected and used to compare proximate composition and energy value with control sample (30% pork backfat). Results: An increase in konjac gel resulted in higher values of LAB, total acidity, and proteolysis index with lower pH and lipid oxidation during 3 days of product fermentation (p<0.05). It was noted that larger weight loss and product shrinkage during fermentation was observed with higher levels of konjac gel (p<0.05). The resulting sausage at day 3 with 15% to 30% konjac gel exhibited higher hardness, cohesiveness, gumminess, springiness, and chewiness than control (p<0.05). The external color of samples with 22.5% to 30% konjac gel were redder than others (p<0.05). Mold, Salmonella spp., Staphylococcus aureus, and Escherichia coli in all finished products were lower than detectable levels. Product with 15% konjac gel had the highest scores of sourness linking and overall acceptability (p<0.05). Conclusion: The product with 15% of konjac gel was the optimum formulation for replacing pork backfat. It had higher sensorial scores of sourness and overall acceptability than control with less negative impact on external appearance (product shrinkage) and weight loss. Moreover, it provided 46% fat reduction and 32% energy reduction than control.
Keywords
Sai Krok E-san; Fermented Sausage; Konjac; Low-fat Meat Product; Healthy Meat Product; Functional Meat;
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1 Visessanguan W, Benjakul S, Smitinont T, Kittikun C, Thepkasikul P, Panya A. Changes in microbiological, biochemical and physico-chemical properties of Nham inoculated with different inoculum levels of Lactobacillus curvatus. LWT-Food Sci Technol 2006;39:814-26. https://doi.org/10.1016/j.lwt.2005.05.006   DOI
2 Thai community product standard. Sandard No. 144-2555:Fermented pork sausages, Sai Krok E-San Mu. Bangkok, Thailand: Thai Industrial Standards Institute; 2012 [cited 2017 Oct 1]. Available from: http://tcps.tisi.go.th/public/StandardList.aspx
3 Thai community product standard. Sandard No. 145-2555:Fermented ground porks, Naem Mu. Bangkok; Thai Industrial Standards Institute; 2012 [cited 2017 Oct 1]. Available from: http://tcps.tisi.go.th/public/StandardList.aspx (In Thai)
4 WHO. Diet, nutrition and the prevention of chronic diseases. Geneva, Switzerland: WHO Library Cataloguing-in-Publication Data; 2003. World Health Organization Technical Report Series; 916.
5 Grasso S, Brunton NP, Lyng JG, Lalor F, Monahan FJ. Healthy processed meat products - Regulatory, reformulation and consumer challenges. Trends Food Sci Technol 2014;39:4-17. https://doi.org/10.1016/j.tifs.2014.06.006   DOI
6 Triki M, Herrero AM, Jimenez-Colmenero F, Ruiz-Capillas C. Effect of preformed konjac gels, with and without olive oil, on the technological attributes and storage stability of merguez sausage. Meat Sci 2013;93:351-60. https://doi.org/10.1016/j.meatsci.2012.10.004   DOI
7 Williams CJ. Medicinal plants in Australia. Vol. 3. Plants, potions and poisons. Kenthurst, Australia: Rosenberg Publishing;2012.
8 Ruiz-Capillas C, Triki M, Herrero AM, Rodriguez-Salas L, Jimenez-Colmenero F. Konjac gel as pork backfat replacer in dry fermented sausages: Processing and quality characteristics. Meat Sci 2012;92:144-50. https://doi.org/10.1016/j.meatsci.2012.04.028   DOI
9 Osburn, WN, Keeton JT. Evaluation of low-fat sausage containing desinewed lamb and konjac gel. Meat Sci 2004;68:221-33. https://doi.org/10.1016/j.meatsci.2004.03.001   DOI
10 Jimenez-Colmenero F, Cofrades S, Lopez-Lopez I, Ruiz-Capillas C, Pintado T, Solas MT. Technological and sensory characteristics of reduced/low-fat, low-salt frankfurters as affected by the addition of konjac and seaweed. Meat Sci 2010;84:356-63. https://doi.org/10.1016/j.meatsci.2009.09.002   DOI
11 Sethakul J, Sivapirunthep P. Meat processing. Bangkok, Thailand: Mean Service Supply Ltd., Part; 2012.
12 Chin KB, Keeton JT, Miller RK, Longnecker MT, Lamkey JW. Evaluation of konjac blends and soy protein isolate as fat replacement in low-fat bologna. J Food Sci 2000;65:756-63. https://doi.org/10.1111/j.1365-2621.2000.tb13582.x   DOI
13 Osburn WN, Keeton JT. Konjac flour gel as fat substitute in low-fat prerigor fresh pork sausage. J Food Sci 1994;59:484-9. https://doi.org/10.1111/j.1365-2621.1994.tb05543.x   DOI
14 Berry BW, Bigner ME. Use of carrageenan and konjac flour gel in low-fat restructured pork nuggets. Food Res Int 1996;29:355-62. https://doi.org/10.1016/0963-9969(96)00019-1   DOI
15 Kheadr E. Impact of acid and oxgall on antibiotic susceptibility of probiotic Lactobacilli. Afr J Agric Res 2006;1:172-81.
16 Latimer GW. AOAC International. Official methods of analysis of AOAC international. 19th ed. Gaithersburg, MD, USA: AOAC International; 2012.
17 Morrissey MT, Wu JW, Lin D, An H. Protease inhibitor effects on torsion measurements and autolysis of Pacific whiting surimi. J Food Sci 1993;58:1050-4. https://doi.org/10.1111/j.1365-2621.1993.tb06109.x   DOI
18 Buege JA, Aust SD. Microsomal lipid peroxidation. Methods Enzymol 1978;52:302-4. https://doi.org/10.1016/S0076-6879(78)52032-6   DOI
19 ISO 6579: 2002. Microbiology of food and animal feeding stuffs-horizontal method for the detection. 4th ed. Geneva, Switzerland: International Organization for Standardization;2002. p. 9.
20 Bennett RW, Lancette GA. Staphylococcus aureus. Bacteriological analytical manual (BAM) [Internet]. Silver Spring, MD, USA: U.S. Food and Drug Administration; 2001 [cited 2016 Mar 23]. Available from: https://www.fda.gov/food/foodscienceresearch/laboratorymethods/ucm071429.htm
21 Parry JM. Konjac glucomannan. In: Imeson A, editor. Food stabilizers, thickeners and gelling agents. Singapore: Blackwell Publishing Ltd; 2010. p. 198-215.
22 Feng P, Weagant SD, Grant MA, Burkhardt W. Enumeration of Escherichia coli and the coliform bacteria in food. Bacteriological analytical manual (BAM) [Internet]. Silver Spring, MD, USA: U.S. Food and Drug Administration; 2002 [cited 2016 Mar 23]. Available from: https://www.fda.gov/Food/FoodScienceResearch/LaboratoryMethods/ucm064948.htm.
23 Valyasevi R, Smitinont T, Visessanguan W, et al. The fermentation rate and production of metabolic compounds during the fermentation of cultured Nham. Pathumthani, Thailand: National Center for Genetic Engineering and Biotechnology; 2006.
24 Gullon P, Gullon B, Moure A, Jose Luis A, Herminia D. Manufacture of prebiotics from biomass sources. In: Charalampopoulos D, Rastall R, editors. Prebiotics and probiotics science and technology. New York, USA: Springer; 2009. p. 535-89.
25 Huff-Lonergan E. Fresh meat water-holding capacity. In: Kerry JP, Ledward DA, editors. Improving the sensory, and nutritional quality of fresh meat. Cambridge, England: Woodhead Publishing Limited; 2009. p. 147-60.
26 Heinz G, Hautzinger P. Meat processing technology for smallto medium-scale producers [Internet]. Bangkok, Thailand: Food and Agriculture Organization of the United Nations Regional Office for Asia and Pacific; 2007 [cited 2017 Oct 1]. Available from: http://www.fao.org/docrep/010/ai407e/AI407E05.htm
27 Stahnke LH. Aroma components from dried sausages fermented with Staphylococcus xylosus. Meat Sci 1994;38:39-53. https://doi.org/10.1016/0309-1740(94)90094-9   DOI
28 Toldra F, Sanz Y, Flores M. Meat fermentation technology. In: Damodaran S, Paraf A, editors. Meat science and applications. New York, USA: Marcel Dekker; 2001. p. 537-61.
29 Fernandez M, Ordonez JA, Bruna JM, Herranz B, de la Hoz L. Accelerated ripening of dry fermented sausages. Trends Food Sci Technol 2000;11:201-9. https://doi.org/10.1016/S0924-2244(00)00077-7   DOI
30 Molly K, Demeyer D, Johansson G, Raemaekers M, Ghistelinck M, Geenen I. The importance of meat enzymes in ripening and flavour generation in dry fermented sausages. Food Chem 1997;59:539-45. https://doi.org/10.1016/S0308-8146(97)00004-6   DOI
31 Cook PE. Fungal ripened meats and meat products. In: Campbell-Platt G, Cook PE, editors. Fermented meats. London, UK: Blackie Academic and Professional; 1995. p. 110-29.
32 Wiriyacharee P. Using mixed starter culture for Thai Nham. In: Gaden EL, Bokanga M, Harlander S, Hesseltine CW, Steinkraus KH, editors. Applications of biotechnology to traditional fermented foods. Washington DC, USA: National Academy Press; 1992. p. 121-7.
33 Liaros NG, Katsanidis E, Bloukas JG. Effect of the ripening time under vacuum and packaging film permeability on processing and quality characteristics of low-fat fermented sausages. Meat Sci 2009;83:589-98. https://doi.org/10.1016/j.meatsci.2009.07.006   DOI
34 Selgas MD, Garcia ML. Yeasts. In: Toldra, F, Hui YH, Astiasaran I, Sebranek JG, Talon R. editor. Handbook of fermented meat and poultry, 2nd ed. New York, USA: Wiley-Blackwell; 2015.p. 139-46.
35 Martinez-Munoz GA, Kane P. Vacuolar and plasma membrane proton pumps collaborate to achieve cytosolic pH homeostasis in yeast. J Biol Chem 2008;283:20309-19. https://doi.org/10.1074/jbc.M710470200   DOI
36 Food and Drug Administration, Thailand. Notification of the Ministry of Public Health No. 182 B.E. 2541. Nutritional label [Internet]. Nonthaburi, Thailand: Food and Drug Administration, Ministry of Public Health; 2014 [cited 2017 Oct 1]. Available from: http://food.fda.moph.go.th/law/data/announ_moph/P182.pdf
37 Osei Abunyewa AA, Laing E, Hugo A, Viljoen BC. The population change of yeasts in commercial salami. Food Microbiol 2000;17:429-38. https://doi.org/10.1006/fmic.1999.0333   DOI
38 Mendoza E, Garcia ML, Casas C, Selgas MD. Inulin as fat substitute in low fat, dry fermented sausages. Meat Sci 2001; 57:387-93. https://doi.org/10.1016/S0309-1740(00)00116-9   DOI
39 Jimenez-Colmenero F, Carballo J, Cofrades S. Healthier meat and meat products: their role as functional foods. Meat Sci 2001;59:5-13. https://doi.org/10.1016/S0309-1740(01)00053-5   DOI
40 Krairojananan P, Tanasupawat S, Noonpakdee W, Valyasevi R. Catalases of bacteria isolated from Thai fermented foods. Food Biotechnol 1998;12:221-38. https://doi.org/10.1080/08905439809549954   DOI
41 Visessanguan W, Benjakul S, Riebroy S, Yarchai M, Tapingkae W. Changes in lipid composition and fatty acid profile of Nham, a Thai fermented pork sausage, during fermentation. Food Chem 2006;94:580-8. https://doi.org/10.1016/j.foodchem.2004.11.051   DOI
42 Phithakpol B, Varanyanond W, Reungmaneepaitoon S, Wood H. The traditional fermented foods of Thailand. Bangkok, Thailand: Institute of Food Research and Product Development, Kasetsart University; 1995.
43 Visessanguan W, Benjakul S, Riebroy S, Thepkasikul P. Changes in composition and functional properties of proteins and their contributions to Nham characteristics. Meat Sci 2004;66:579-88. https://doi.org/10.1016/S0309-1740(03)00172-4   DOI