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http://dx.doi.org/10.5713/ajas.17.0170

Quality of steak restructured from beef trimmings containing microbial transglutaminase and impacted by freezing and grading by fat level  

Sorapukdee, Supaluk (Department of Animal Production Technology and Fisheries, Faculty of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang)
Tangwatcharin, Pussadee (Department of Animal Production Technology and Fisheries, Faculty of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.31, no.1, 2018 , pp. 129-137 More about this Journal
Abstract
Objective: The objective of this research was to evaluate the physico-chemical, microbiological and sensorial qualities of restructured steaks processed from beef trimmings (grade I and II) and frozen beef (fresh beef as control and frozen beef). Methods: Beef trimmings from commercial butcher were collected, designated into 4 treatments differing in beef trimmings grade and freezing, processed into restructured steaks with 1% microbial transglutaminase and then analyzed for product quality. Results: The results showed that all meat from different groups could be tightly bound together via cross-linking of myosin heavy chain and actin as observed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Microbial counts of psychrotrophic and mesophilic bacteria were not affected by treatments (p>0.05), and no detectable of thermophilic bacteria were found. Regarding effect of beef trimmings grade, steaks made from beef trimmings grade II (16.03% fat) showed some superior sensorial qualities including higher tenderness score (p<0.05) and tendency for higher scores of juiciness and overall acceptability (p<0.07) than those made from beef trimmings grade I (2.15% fat). Moreover, a hardness value from texture profile analysis was lower in steaks processed from beef trimmings grade II than those made from grade I (p<0.05). Although some inferior qualities in terms of cooking loss and discoloration after cooking were higher in steaks made from beef trimmings grade II than those made from beef trimmings grade I (p<0.05), these differences did not affect the sensory evaluation. Frozen beef improved the soft texture and resulted in effective meat binding as considered by higher cohesiveness and springiness of the raw restructured product as compared to fresh beef (p<0.05). Conclusion: The results indicated the most suitable raw beef for producing restructured steaks without detrimental effect on product quality was beef trimmings grade II containing up to 17% fat which positively affected the sensory quality and that frozen beef trimmings increased tenderness and meat binding of restructured beef steaks.
Keywords
Restructured Meat; Transglutaminase; Beef; Fat Content; Freezing;
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1 Neely TR, Lorenzen CL, Miller RK, et al. Beef customer satisfaction: Role of cut, USDA quality grade, and city on in-home consumer ratings. J Anim Sci 1998;76:1027-33.
2 Wheeler TL, Miller RK, Savell JW, Cross HR. Palatability of chilled and frozen beef steaks. J Food Sci 1990;55:301-3.   DOI
3 Lagerstedt A, Enfalt L, Johansson L, Lundstrom K. Effect of freezing on sensory quality, shear force and water loss in beef M. longissimus dorsi. Meat Sci 2008;80:457-61.   DOI
4 Latimer GW. AOAC International. Official methods of AOAC International. 19th ed. Gaithersburg, MD, USA: AOAC International; 2012.
5 Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage. Nature 1970;227:680-5.   DOI
6 Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193:265-75.
7 Bourne MC. Texture profile analysis. Food Technol 1978;32:62-5.
8 Downes FP, Ito K. Compendium of methods for the microbiological examination of foods. 4th ed. Washington, DC, USA: American Public Health Association; 2001.
9 SAS Institute Inc. SAS Version 9.0. Cary, NC, USA: SAS Institute; 2002.
10 Daszkiewicz T, Wajda S, Kondratowicz J. Physico-chemical and sensory properties of meat from Black-and-White and Black-and-White$\times$ Limousine heifers differing in intramuscular fat content. Anim Sci Pap Rep 2005;23:181-7.
11 Corley RB. A guide to methods in the biomedical sciences. New York, NY, USA: Springer Science+Business Media, Inc.; 2005.
12 Chaijan M, Panpipat W. Gel-forming ability of mackerel (Rastrelliger Branchysoma) protein isolate as affected by microbial transglutaminas. Walailak J Sci Technol 2010;7:41-9.
13 Qin XS, Luo SZ, Cai J, et al. Effects of microwave pretreatment and transglutaminase crosslinking on the gelation properties of soybean protein isolate and wheat gluten mixtures. J Sci Food Agric 2016;96: 3559-66.   DOI
14 Kumazawa Y, Seguro K, Takamura M, Motoki M. Formation of $\varepsilon$-($\gamma$-glutamyl) lysine cross-link in cured horse mackerel meat induced by drying. J Food Sci 1993;58:1062-4.   DOI
15 Tammatinna A, Benjakul S, Visessanguan W, Tanaka M. Gelling properties of white shrimp (Penaeus vannamei) meat as influenced by setting condition and microbial tranglutaminase. LWT Food Sci Technol 2007;40:1489-97.   DOI
16 Troutt ES, Hunt MC, Johnson DE, et al. Chemical, physical and sensory characterisation of ground beef containing 5 to 30 per cent fat. J Food Sci 1992;57:25-9.
17 Yokoyama K, Nio N, Kikuchi Y. Properties and applications of microbial transglutaminase. Appl Microbiol Biotechnol 2004;64:447-54.   DOI
18 Tornberg E, Olsson A, Persson K. A comparison in fat holding between hamburgers and emulsion sausages. In: Proceeding of the 35th International Congress of Meat Science and Technology 1989; 1989 August 20-25; Copenhagen, Denmark. pp. 752-9.
19 Desmond E, Troy DJ. Nutrient claims on packaging. In: Jensen WK, Devine C, Dikeman M, editors. Encyclopedia of meat sciences. Oxford, UK: Elsevier; 2004. p. 903-9.
20 Utrera M. Morcuende D, Estevez M. Fat content has a significant impact on protein oxidation occurred during frozen storage of beef patties. LWT Food Sci Technol 2014;56:62-8.   DOI
21 Jay JM, Vilai JP, Hughes ME. Profile and activity of the bacterial biota of ground beef held from freshness to spoilage at $5-7^{\circ}C$. Inter J Food Microbiol 2003;81:105-11.   DOI
22 Leygonie C, Britz TJ, Hoffman LC. Impact of freezing and thawing on the quality of meat: review. Meat Sci 2012;91:93-8.   DOI
23 Canto ACVCS, Costa Lima BRC, Suman SP, et al. Physico-chemical and sensory attributes of low-sodium restructured caiman steaks containing microbial transglutaminase and salt replacers. Meat Sci 2014;96:623-32.   DOI
24 Herrero AM, Cambero MI, Ordonez JA, de la Hoz L, Carmona P. Raman spectroscopy study of the structural effect of microbial transglutaminase on meat systems and its relationship with textural characteristics. Food Chem 2008;109:25-32.   DOI
25 Ercolini D, Russo F, Nasi A, Ferranti P, Villani F. Mesophilic and psychrotrophic bacteria from meat and their spoilage potential in vitro and in beef. Appl Environ Microbiol 2009;75:1990-2001.   DOI
26 Gill CO, Newton KG. The ecology of bacterial spoilage of fresh meat at chill temperatures. Meat Sci 1978:2:207-17.   DOI
27 Berry BW, Smith JJ, Secrist JL. Effects of fat level on sensory, cooking and instron properties of restructured beef steaks. J Anim Sci 1985; 60:434-9.   DOI
28 Iida F, Saitou K, Kawamura T, Yamaguchi S, Nishimura T. Effect of fat content on sensory characteristics of marbled beef from Japanese Black steers. Anim Sci J 2015;86:707-15.   DOI
29 Koohmaraie M. Meat tenderness and muscle growth: is there any relationship. Meat Sci 2002;62:345-52.   DOI
30 Rahman MH, Hossain MM, Rahman SM, Hashem MA, Oh DH. Effect of repeated freeze-thaw cycles on beef quality and safety. Korean J Food Sci Anim Resour 2014;34:482-95.   DOI
31 Farouk MM, Zhang SX, Cummings T. Effect of muscle fiber/fiberbundle alignment on physical and sensory properties of restructured beef steaks. J Muscle Food 2005;16:256-73.
32 Office of Agricultural Economic. Situation of agricultural products and trends in Thailand [Internet]. Office of Agricultural Economic; 2015 [cited 2015 Feb 28]. Available from: http://www.oae.go.th/download/document_tendency/journalofecon2558.pdf
33 Kuraishi C, Sakamoto J, Yamazaki K, et al. Production of restructured meat using microbial transglutaminase without salt or cooking. J Food Sci 1997;62:488-90.
34 Lee HG, Lanier TC. The role of covalent cross-linking in the texturizing of muscle protein sols. J Muscle Foods 1995;6:125-38.
35 Antequera T, Perez-Palacios T, Rodas E, Rodriguez M, Cordoba JJ. Effect of muscle type and frozen storage on the quality parameters of Iberian restructured meat preparations. Food Sci Technol Int 2014; 20:543-54.   DOI
36 Sen AR, Karim SA. Effect of meat particle size on quality attributes of restructured mutton steaks. J Food Sci Technol 2003;40:423-5.
37 Sethakul J, Sivapirunthep P. Meat processing. Bangkok, Thailand: Mean Service Supply Ltd., Part; 2012.
38 Farouk MM, Hall WK, Wieliczko KJ, Swan JE. Processing time and binder effect on the quality of restructured rolls from hot-boned beef. J Muscle Food 2005;16:318-29.   DOI
39 Gadekar YP, Sharma BD, Shinde AK, Mendiratta SK. Restructured meat products - production, processing and marketing: A review. Indain J Small Rumin 2015;21:1-12.   DOI
40 Heinz G, Hautzinger P. Meat processing technology for small-to medium-scale producers [Internet]. Bangkok: Food and Agriculture Organization of the United Nations Regional Office for Asia and Pacific; 2007 [cited 2015 Feb 28]. Available from: http://www.fao.org/docrep/010/ai407e/AI407E05.htm
41 Savell JW, Branson RE, Cross HR, et al. National consumer retail beef study: Palatability evaluations of beef loin steaks that differed in marbling. J Food Sci 1987;52:517-9.   DOI