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http://dx.doi.org/10.5713/ajas.18.0173

Association of polymorphisms in Pit-1 gene with growth and feed efficiency in meat-type chickens  

Jin, Sihua (College of Animal Science and Technology, Anhui Agricultural University)
He, Tingting (College of Animal Science and Technology, Anhui Agricultural University)
Yang, Lei (College of Animal Science and Technology, Anhui Agricultural University)
Tong, Yucui (College of Animal Science and Technology, Anhui Agricultural University)
Chen, Xingyong (College of Animal Science and Technology, Anhui Agricultural University)
Geng, Zhaoyu (College of Animal Science and Technology, Anhui Agricultural University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.31, no.11, 2018 , pp. 1685-1690 More about this Journal
Abstract
Objective: The pituitary specific transcription factor-1 (Pit-1) gene is responsible for pituitary development and growth hormone expression and is regarded as a pivotal candidate gene for growth and production in chickens. Therefore, the aim of this study was to investigate the association of Pit-1 polymorphisms with growth and feed efficiency traits in yellow meat-type chickens. Methods: In the present study, five single nucleotide polymorphisms (SNPs) of Pit-1 were selected and genotyped by high-throughput matrix-assisted laser desorption-ionization time-of-flight mass spectrometry in 724 meat-type chickens. Results: Association analysis showed that rs13687126 of Pit-1 was strongly associated with body weight gain (BWG) and feed intake (FI) (p<0.05), and that rs13687128 was significantly correlated with body weight at 70 days of age (BW70), BWG and feed conversion ratio (FCR) (p<0.05). SNP rs13905622 was strongly related to BW70 and FCR (p<0.05). Furthermore, birds with the GG genotype of rs13687126 had larger BWG and FI than those with the AG genotype (p<0.05). Individuals with the TT genotype of rs13687128 were significantly higher BW70 and BWG than those of the CT and CC genotype, while FCR was just the opposite (p<0.05). For rs13905622, the AA chickens showed strongly larger BW70 and lower FCR compared with the AT and TT chickens (p<0.05). Additionally, an ACA haplotype based on rs13687126, rs13687128, and rs13905622 had significant effects on BW70 and FCR (p<0.05). Conclusion: Our studies thus provide crucial evidence for the relationship between polymorphisms of Pit-1 and growth and feed efficiency traits which may be useful for meat-type chicken breeding programs.
Keywords
Chicken; Pit-1; Single Nucleotide Polymorphism; Feed Conversion Ratio; Association;
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