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http://dx.doi.org/10.5713/ajas.18.0045

In vitro evaluation of Rhus succedanea extracts for ruminants  

Kim, Do Hyung (Department of Animal Science, Gyeongbuk Provincial College)
Lee, Shin Ja (Institute of Agriculture and Life Science and University-Centered Labs, Gyeongsang National University)
Oh, Da Som (Division of Applied Life Science (BK21 Program) and Institute of Agriculture & Life Science (IALS), Gyeongsang National University)
Lee, Il Dong (Division of Applied Life Science (BK21 Program) and Institute of Agriculture & Life Science (IALS), Gyeongsang National University)
Eom, Jun Sik (Division of Applied Life Science (BK21 Program) and Institute of Agriculture & Life Science (IALS), Gyeongsang National University)
Park, Ha Young (Department of Pathology, Busan Paik Hospital, Inje University College of Medicine)
Choi, Seong Ho (Department of Animal Science, Chungbuk National University)
Lee, Sung Sill (Division of Applied Life Science (BK21 Program) and Institute of Agriculture & Life Science (IALS), Gyeongsang National University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.31, no.10, 2018 , pp. 1635-1642 More about this Journal
Abstract
Objective: This study was conducted to evaluate the effects of Rhus succedanea extract addition on in vitro ruminal fermentation and microbial growth. Methods: Two ruminally-fistulated steers consuming 600 g/kg timothy- and 400 g/kg cracked corn-based concentrate with free access to water and mineral block were used as rumen fluid donors. In vitro batch fermentation, with timothy as a substrate, was conducted for up to 72 h, with Rhus succedanea extracts added to achieve final concentrations of 0, 10, 30, 50, 70, and 90 mg/L. Results: Effective dry matter (DM) degradability rate linearly decreased (p = 0.046) depending on extract dosing levels. Total gas production after 24 to 72 h incubation tended to decrease following extract addition, beginning with 50 mg/L starting dose (significance of quadratic effects: p = 0.006, p<0.001, and p = 0.008 for 24, 48, and 72 h, respectively). Methane production decreased depending on dosing levels following 24 h (p<0.05) and 48 h (p<0.005) incubations and was the lowest with the 50 mg/L dose. The Rhus succedanea extracts increased the abundance of Fibrobacter succinogenes (p<0.05) and Ruminococcus flavefaciens (p = 0.0597) and decreased the abundance of methanogenic archaea (p<0.05) following 24 h incubation. Conclusion: Rhus succedanea was shown to reduce methane production and increase cellulolytic bacteria without any signs of toxic effects and with a minor effect on DM degradability.
Keywords
Rhus succedanea Extracts; In vitro Ruminal Fermentation; Microbial Growth;
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