Browse > Article
http://dx.doi.org/10.5713/ajas.16.0708

Effect of alcohol dehydrogenase 1C (ADH1C) genotype on vitamin A restriction and marbling in Korean native steers  

Peng, Dong Qiao (Department of Animal Science and Technology, College of Animal Bioscience and Technology, Konkuk University)
Jung, U Suk (Department of Animal Science and Technology, College of Animal Bioscience and Technology, Konkuk University)
Lee, Jae Sung (Department of Animal Science and Technology, College of Animal Bioscience and Technology, Konkuk University)
Kim, Won Seob (Department of Animal Science and Technology, College of Animal Bioscience and Technology, Konkuk University)
Jo, Yong Ho (Department of Animal Science and Technology, College of Animal Bioscience and Technology, Konkuk University)
Kim, Min Jeong (Department of Animal Science and Technology, College of Animal Bioscience and Technology, Konkuk University)
Oh, Young Kun (Animal Nutrition and Physiology team, Department of Animal Biotechnology and Environment, National Institute of Animal Science RDA)
Baek, Youl Chang (Animal Nutrition and Physiology team, Department of Animal Biotechnology and Environment, National Institute of Animal Science RDA)
Hwang, Seong Gu (Department of Animal Life and Environment Science, Hankyong National University)
Lee, Hong Gu (Department of Animal Science and Technology, College of Animal Bioscience and Technology, Konkuk University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.30, no.8, 2017 , pp. 1099-1104 More about this Journal
Abstract
Objective: This work was to find the correlation of alcohol dehydrogenase 1C (ADH1C) genotype with vitamin A reduction and carcass traits during the vitamin A restriction period. Methods: In study 1, 60 Korean native steers were fed a diet (890 IU/kg) with 8,000 IU and 0 IU of supplemental premix vitamin A/kg of dry matter (DM) for control and treatment group, respectively. The levels of serum vitamin A were analyzed through high preparative performance liquid chromatography, and the ADH1C genotype was analyzed based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP; 78.1% TT type, 21.9% TC type); however, CC type was not found. Then, the interaction between ADH1C and carcass traits on the vitamin A restriction was investigated in study 2. A total of 136 Korean native steers were fed a diet that included 930 IU/kg vitamin A of DM. Results: Serum vitamin A in treatment was reduced to 112.4 IU/dL in steers with TT type of ADH1C, while for steers with TC type the concentration of serum vitamin A was dropped to 79.5 IU/dL (p<0.1) in study 1. This showed that TC type had the potential to lower serum vitamin A concentration during vitamin A restriction compared to TT type. In study 2 we found that eye muscle area, marbling and carcass weight in Korean native steers with TC type were higher than in steers with TT type (p<0.05). Conclusion: The interaction between vitamin A restriction and TC type of ADH1C gene could have the potential of increasing the marbling in Korean native steers. These results indicated that steers with TC type of the ADH1C gene were more sensitive to the change of serum vitamin A than TT types. Furthermore, this finding has the potential to enable a higher marbling score under the condition of vitamin A restriction in Korean native steers.
Keywords
Vitamin A Restriction; Alcohol Dehydrogenase 1C (ADH1C); Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP); Marbling; Korean Native Steers;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Shiranita K, Hayashi K, Otsubo A, Miyajima T, Takiyama R. Grading meat quality by image processing. Pattern Recogn 2000;33:97-104.   DOI
2 Oka A, Maruo Y, Miki T, Yamasaki T, Saito T. Influence of vitamin A on the quality of beef from the Tajima strain of Japanese black cattle. Meat Sci 1998;48:159-67.   DOI
3 Gibb D, Van Herk F, Mir P, Loerch S, McAllister T. Removal of supplemental vitamin A from barley-based diets improves marbling in feedlot heifers. Canadian J Anim Sci 2011;91:669-74.   DOI
4 Ward AK, McKinnon JJ, Hendrick S, Buchanan FC. The impact of vitamin A restriction and ADH1C genotype on marbling in feedlot steers. J Anim Sci 2012;90:2476-83.   DOI
5 Molotkov A, Fan X, Duester G. Excessive vitamin A toxicity in mice genetically deficient in either alcohol dehydrogenase Adh1 or Adh3. Eur J Biochem 2002;269:2607-12.   DOI
6 Molotkov A, Duester G. Genetic evidence that retinaldehyde dehydrogenase Raldh1 (Aldh1a1) functions downstream of alcohol dehydrogenase Adh1 in metabolism of retinol to retinoic acid. J Biol Chem 2003;278:36085-90.   DOI
7 Moon S, Hwang I, Jin S, Lee J, Joo S, Park G. Carcass traits determining quality and yield grades of Hanwoo steers. Asian-Australas J Anim Sci 2003;16:1049-54.   DOI
8 Pickworth CL, Loerch SC, Fluharty FL. Restriction of vitamin A and D in beef cattle finishing diets on feedlot performance and adipose accretion. J Anim Sci 2012;90:1866-78.   DOI
9 Gorocica-Buenfil MA, Fluharty FL, Loerch SC. Effect of vitamin A restriction on carcass characteristics and immune status of beef steers. J Anim Sci 2008;86:1609-16.   DOI
10 Adachi K, Kawano H, Tsuno K, et al. Relationship between serum biochemical values and marbling scores in Japanese Black steers. J Vet Med Sci 1999;61:961-4.   DOI
11 National Research Council (US). Subcommittee on beef cattle nutrition. Nutrient requirements of beef cattle. 7th rev. ed. Washington, DC:National Academy Press; 1996.
12 Krone KG, Ward AK, Madder KM, et al. Interaction of vitamin A supplementation level with ADH1C genotype on intramuscular fat in beef steers. Animal 2016;10:403-9.   DOI
13 Kiefer FW, Orasanu G, Nallamshetty S, et al. Retinaldehyde dehydrogenase 1 coordinates hepatic gluconeogenesis and lipid metabolism. Endocrinology 2012;153:3089-99.   DOI
14 Napoli JL, Boerman MH, Chai X, Zhai Y, Fiorella PD. Enzymes and binding proteins affecting retinoic acid concentrations. J Steroid Biochem Mol Biol 1995;53:497-502.   DOI
15 Kim DM, Choi HR, Park A, et al. Retinoic acid inhibits adipogenesis via activation of Wnt signaling pathway in 3T3-L1 preadipocytes. Biochem Biophys Res Commun 2013;434:455-9.   DOI
16 Sagara C, Takahashi K, Kagechika H, Takahashi N. Molecular mechanism of 9-cis-retinoic acid inhibition of adipogenesis in 3T3-L1 cells. Biochem Biophys Res Commun 2013;433:102-7.   DOI
17 Berry DC, Noy N. All-trans-retinoic acid represses obesity and insulin resistance by activating both peroxisome proliferation-activated receptor beta/delta and retinoic acid receptor. Mol Cell Biol 2009;29:3286-96.   DOI
18 Amengual J, Ribot J, Bonet ML, Palou A. Retinoic acid treatment enhances lipid oxidation and inhibits lipid biosynthesis capacities in the liver of mice. Cell Physiol Biochem 2010;25:657-66.   DOI
19 Mizoguchi Y, Moriya M, Taniguchi D, Hasegawa A. Effect of retinoic acid on gene expression profiles of bovine intramuscular preadipocytes during adipogenesis. Anim Sci J 2014;85:101-11.   DOI