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http://dx.doi.org/10.5713/ajas.16.0773

Effects of sodium diacetate on the fermentation profile, chemical composition and aerobic stability of alfalfa silage  

Yuan, XianJun (Institute of Ensiling and Processing of Grass, Nanjing Agricultural University)
Wen, AiYou (Institute of Ensiling and Processing of Grass, Nanjing Agricultural University)
Desta, Seare T. (Institute of Ensiling and Processing of Grass, Nanjing Agricultural University)
Wang, Jian (Institute of Ensiling and Processing of Grass, Nanjing Agricultural University)
Shao, Tao (Institute of Ensiling and Processing of Grass, Nanjing Agricultural University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.30, no.6, 2017 , pp. 804-810 More about this Journal
Abstract
Objective: The objective of this study was to evaluate the effect of sodium diacetate (SDA) on fermentation profile, chemical composition and aerobic stability of alfalfa (Medicago sativa L.) silage. Methods: Fresh alfalfa was ensiled with various concentrations of SDA (0, 3, 5, 7, and 9 g/kg of fresh forage). After 60 days of the ensiling, the samples were collected to examine the fermentative quality, chemical composition and aerobic stability. Results: The application of SDA significantly (p<0.05) decreased silage pH with the lowest value in silage with 7 g/kg of SDA. The proliferations of enterobacteria, yeasts, molds and clostridia were inhibited by SDA, resulted in lower ethanol, propionic and butyric acid concentrations and dry matter loss in SDA treated silages than control. The increasing SDA linearly decreased free amino acid N (p<0.001), ammonia N (p = 0.018) and non-protein N (p<0.001), while linearly increased water soluble carbohydrate (p<0.001) and peptide N (p<0.001). It is speculated that SDA accelerated the shift from homofermentative to heterofermentative lactic acid bacteria during the silage fermentation, indicated by lower lactic acid production in SDA-9 than SDA-7 silages after 60 days of ensiling. Alfalfa silages treated with SDA at 7 g/kg had highest Flieg's point and remained stable more than 9 d during aerobic exposure under humid and hot conditions in southern China. Conclusion: SDA may be used as an additive for alfalfa silages at a level of 7 g/kg.
Keywords
Alfalfa; Fermentation Quality; Sodium Diacetate; Silage;
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1 Knicky M, Sporndly R. Sodium benzoate, potassium sorbate and sodium nitrite as silage additives. J Sci Food Agric 2009;89:2659-67.   DOI
2 Jones BA, Hatfield RD, Muck RE. Characterization of Proteolysis in alfalfa and red clover. Crop Sci 1995;35:537-41.   DOI
3 Ogunade IM, Kim DH, Jiang Y, et al. Control of Escherichia coli O157:H7 in contaminated alfalfa silage: Effects of silage additives. J Dairy Sci 2016;99:4427-36.   DOI
4 Nkosi BD, Meeske R, Langa T, et al. Effects of ensiling forage soybean (Glycine max (L.) Merr.) with or without bacterial inoculants on the fermentation characteristics, aerobic stability and nutrient digestion of the silage by Damara rams. Small Rumin Res 2016;134:90-6.   DOI
5 Kung Jr L, Ranjit NK. The effect of lactobacillus buchneri and other additives on the fermentation and aerobic stability of barley silage. J Dairy Sci 2001;84:1149-55.   DOI
6 Wilkinson JM, Davies DR. The aerobic stability of silage: key findings and recent developments. Grass Forage Sci 2013;68:1-19.   DOI
7 Guo X, Zhou H, Yu Z, Zhang Y. Changes in the distribution of nitrogen and plant enzymatic activity during ensilage of lucerne treated with different additives. Grass Forage Sci 2007;62:35-43.   DOI
8 Broderick GA, Kang JH. Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. J Dairy Sci 1980;63:64-75.   DOI
9 Licitra G, Hernandez TM, Van Soest PJ. Standardization of procedures for nitrogen fractionation of ruminant feeds. Anim Feed Sci Technol 1996;57:347-58.   DOI
10 Muck R. Dry matter level effects on alfalfa silage quality. I. Nitrogen transformations. Trans ASAE 1987;30:7-14.   DOI
11 Thomas TA. An automated procedure for the determination of soluble carbohydrates in herbage. J Sci Food Agric 1977;28:639-42.   DOI
12 Playne MJ, McDonald P. The buffering constituents of herbage and of silage. J Sci Food Agric 1966;17:264-8.   DOI
13 Moselhy MA, Borba JP, Borba AES. Improving the nutritive value, in vitro digestibility and aerobic stability of Hedychium gardnerianum silage through application of additives at ensiling time. Anim Feed Sci Technol 2015;206:8-18.   DOI
14 Nkosi BD, Meeske R. Effects of ensiling totally mixed potato hash ration with or without a heterofermentative bacterial inoculant on silage fermentation, aerobic stability, growth performance and digestibility in lambs. Anim Feed Sci Technol 2010;161:38-48.   DOI
15 Denek N, Can A, Avci M, Aksu T, Durmaz H. The effect of molassesbased pre-fermented juice on the fermentation quality of first-cut lucerne silage. Grass Forage Sci 2011;66:243-50.   DOI
16 Muck RE. Factors influencing silage quality and their implications for management. J Dairy Sci 1988;71:2992-3002.   DOI
17 Woolford MK. Microbiological screening of the straight chain fatty acids (c1-c12) as potential silage additives. J Sci Food Agric 1975;26: 219-28.   DOI
18 Dellaglio F, Torriani S. DNA-DNA homology, physiological characteristics and distribution of lactic acid bacteria isolated from maize silage. J Appl Bacteriol 1986;60:83-92.   DOI
19 Castle ME, Watson JN. Silage and milk production: studies with molasses and formic acid as additives for grass silage. Grass Forage Sci 1985;40:85-92.   DOI
20 Yuan X, Yu C, Shimojo M, Shao T. Improvement of fermentation and nutritive quality of straw-grass silage by inclusion of wet hulless-barley distillers' grains in Tibet. Asian-Austral J Anim 2012;25:479-85.   DOI
21 Goeser JP, Heuer CR, Crump PM. Forage fermentation product measures are related to dry matter loss through meta-analysis. Prof Anim Sci 2015;31:137-45.
22 Tabacco E, Righi F, Quarantelli A, Borreani G. Dry matter and nutritional losses during aerobic deterioration of corn and sorghum silages as influenced by different lactic acid bacteria inocula. J Dairy Sci 2011;94:1409-19.   DOI
23 Da Silva TC, Smith ML, Barnard AM, Kung L, Jr. The effect of a chemical additive on the fermentation and aerobic stability of high-moisture corn. J Dairy Sci 2015;98:8904-12.   DOI
24 Fijalkowska M, Pysera B, Lipinski K, Strusinska D. Changes of nitrogen compounds during ensiling of high protein herbages-a review. Ann Anim Sci 2015;15:289-305.   DOI
25 Danner H, Holzer M, Mayrhuber E, Braun R. Acetic acid increases stability of silage under aerobic conditions. Appl Environ Microb 2003;69:562-7.   DOI
26 Silva VP, Pereira OG, Leandro ES, et al. Effects of lactic acid bacteria with bacteriocinogenic potential on the fermentation profile and chemical composition of alfalfa silage in tropical conditions. J Dairy Sci 2016;99:1895-902.   DOI
27 Cazzato E, Laudadio V, Corleto A, Tufarelli V. Effects of harvest date, wilting and inoculation on yield and forage quality of ensiling safflower (Carthamus tinctorius L.) biomass. J Sci Food Agric 2011;91:2298-302.
28 Oladosu Y, Rafii MY, Abdullah N, et al. Fermentation quality and additives: a case of rice straw silage. Biomed Res Int 2016;ID:7985167.
29 Shockey W, Heinrichs A, Weiss W, Conrad H. Evaluation of sodium diacetate-treated alfalfa silage for lactating dairy cows. J Prod Agric 1990;3:381-5.   DOI