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http://dx.doi.org/10.5713/ajas.16.0637

Expression and regulation of prostaglandin transporters, ATP-binding cassette, subfamily C, member 1 and 9, and solute carrier organic anion transporter family, member 2A1 and 5A1 in the uterine endometrium during the estrous cycle and pregnancy in pigs  

Jang, Hwanhee (Department of Biological Science and Technology, Yonsei University)
Choi, Yohan (Department of Biological Science and Technology, Yonsei University)
Yoo, Inkyu (Department of Biological Science and Technology, Yonsei University)
Han, Jisoo (Department of Biological Science and Technology, Yonsei University)
Kim, Minjeong (Department of Biological Science and Technology, Yonsei University)
Ka, Hakhyun (Department of Biological Science and Technology, Yonsei University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.30, no.5, 2017 , pp. 643-652 More about this Journal
Abstract
Objective: Prostaglandins (PGs) function in various reproductive processes, including luteolysis, maternal pregnancy recognition, conceptus development, and parturition. Our earlier study has shown that PG transporters ATP-binding cassette, subfamily C, member 4 (ABCC4) and solute carrier organic anion transporter family, member 2A1 (SLCO2A1) are expressed in the uterine endometrium in pigs. Since several other PG transporters such as ABCC1, ABCC9, SLCO4C1, and SLCO5A1 are known to be present in the uterine endometrium, this study investigated the expression of these PG transporters in the porcine uterine endometrium and placenta. Methods: Uterine endometrial tissues were obtained from gilts on day (D) 12 and D15 of the estrous cycle and days 12, 15, 30, 60, 90, and 114 of pregnancy. Results: ABCC1, ABCC9, SLCO4C1, and SLCO5A1 mRNAs were expressed in the uterine endometrium, and levels of expression changed during the estrous cycle and pregnancy. Expression of ABCC1 and ABCC9 mRNAs was localized mainly to luminal and glandular epithelial cells in the uterine endometrium, and chorionic epithelial cells during pregnancy. Conceptuses during early pregnancy and chorioallantoic tissues from mid to late pregnancy also expressed these PG transporters. $Estradiol-17{\beta}$ increased the expression of ABCC1 and SLCO5A1, but not ABCC9 and SLCO4C1 mRNAs and increasing doses of $interleukin-1{\beta}$ induced the expression of ABCC9, SLCO4C1, and SLCO5A1 mRNAs in endometrial explant tissues. Conclusion: These data showed that several PG transporters such as ABCC1, ABCC9, SLCO4C1, and SLCO5A1 were expressed at the maternal-conceptus interface, suggesting that these PG transporters may play an important role in the establishment and maintenance of pregnancy by regulating PG transport in the uterine endometrium and placenta in pigs.
Keywords
Pig; Uterus; Endometrium; Prostaglandin; Prostaglandin Transporters;
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  • Reference
1 Schuster VL. Molecular mechanisms of prostaglandin transport. Annu Rev Physiol 1998;60:221-42.   DOI
2 Challis JRG, Matthews SG, Gibb W, Lye SJ. Endocrine and paracrine regulation of birth at term and preterm. Endocr Rev 2000;21:514-50.
3 Spencer TE, Bazer FW. Uterine and placental factors regulating conceptus growth in domestic animals. J Anim Sci 2004;82 E-Suppl:E4-13.
4 Bazer FW, Johnson GA. Pig blastocyst-uterine interactions. Differentiation 2014;87:52-65.   DOI
5 Ziecik AJ. Old, new and the newest concepts of inhibition of luteolysis during early pregnancy in pig. Domest Anim Endocrinol 2002;23:265-75.   DOI
6 Geisert RD, Renegar RH, Thatcher WW, Roberts RM, Bazer FW. Establishment of pregnancy in the pig: I. Interrelationships between preimplantation development of the pig blastocyst and uterine endometrial secretions. Biol Reprod 1982;27:925-39.   DOI
7 Waclawik A. Novel insights into the mechanisms of pregnancy establishment: regulation of prostaglandin synthesis and signaling in the pig. Reproduction 2011;142:389-99.   DOI
8 Seo H, Choi Y, Shim J, Yoo I, Ka H. Prostaglandin transporters ABCC4 and SLCO2A1 in the uterine endometrium and conceptus during pregnancy in pigs. Biol Reprod 2014;90:100.   DOI
9 Schuster VL. Prostaglandin transport. Prostaglandins Other Lipid Mediat 2002;68-69:633-47.   DOI
10 Kim M, Seo H, Choi Y, et al. Analysis of stage-specific gene expression profiles in the uterine endometrium during pregnancy in pigs. PLoS One 2015;10:e0143436.   DOI
11 Samborski A, Graf A, Krebs S, et al. Transcriptome changes in the porcine endometrium during the preattachment phase. Biol Reprod 2013;89:134.
12 Cole SP. Multidrug resistance protein 1 (MRP1, ABCC1), a "multitasking" ATP-binding cassette (ABC) transporter. J Biol Chem 2014;289:30880-8.   DOI
13 Riches Z, Walia G, Berman JM, Wright TE, Collier AC. ATP-binding cassette proteins BCRP, MRP1 and P-gp expression and localization in the human umbilical cord. Xenobiotica 2016;46:548-56.   DOI
14 Lovasz N, Ducza E, Gaspar R, Falkay G. Ontogeny of sulfonylureabinding regulatory subunits of K(ATP) channels in the pregnant rat myometrium. Reproduction 2011;142:175-81.   DOI
15 Kuo KL, Zhu H, McNamara PJ, Leggas M. Localization and functional characterization of the rat Oatp4c1 transporter in an in vitro cell system and rat tissues. PLoS One 2012;7:e39641.   DOI
16 Sebastian K, Detro-Dassen S, Rinis N, et al. Characterization of SLCO5A1/OATP5A1, a solute carrier transport protein with non-classical function. PLoS One 2013;8:e83257.   DOI
17 Hagenbuch B, Meier PJ. Organic anion transporting polypeptides of the OATP/ SLC21 family: phylogenetic classification as OATP/SLCO superfamily, new nomenclature and molecular/functional properties. Pflugers Arch 2004;447:653-65.   DOI
18 Olivier B, Walter W. A simplified in situ hybridization protocol using non-radioactively labeled probes to detect abundant and rare mrnas on tissue sections. Biochemica 1998;1:10-6.
19 Evseenko DA, Paxton JW, Keelan JA. Independent regulation of apical and basolateral drug transporter expression and function in placental trophoblasts by cytokines, steroids, and growth factors. Drug Metab Dispos 2007;35:595-601.   DOI
20 Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 2001;25:402-8.   DOI
21 Olbrich HG, Michaelis H, Vandeplassche G, et al. Ultrastructural calcium distribution and myocardial calcium content in human idiopathic dilated cardiomyopathy. Cardiovasc Pathol 1993;2:127-36.   DOI
22 Aye IL, Paxton JW, Evseenko DA, Keelan JA. Expression, localisation and activity of ATP binding cassette (ABC) family of drug transporters in human amnion membranes. Placenta 2007;28:868-77.   DOI
23 Lybaert P, Hoofd C, Guldner D, et al. Detection of K(ATP) channels subunits in human term placental explants and evaluation of their implication in human placental lactogen (hPL) and human chorionic gonadotropin (hCG) release. Placenta 2013;34:467-73.   DOI
24 Blitek A, Waclawik A, Kaczmarek MM, et al. Expression of cyclooxygenase-1 and -2 in the porcine endometrium during the oestrous cycle and early pregnancy. Reprod Domest Anim 2006;41:251-7.   DOI
25 Edgerton LA, Kaminski MA, Silvia WJ. Changes in uterine secretion of prostaglandin F2 alpha in response to oxytocin during the estrous cycle, early pregnancy, and estrogen-induced pseudopregnancy in swine. Biol Reprod 1996;55:657-62.   DOI
26 Seo H, Choi Y, Shim J, Yoo I, Ka H. Comprehensive analysis of prostaglandin metabolic enzyme expression during pregnancy and the characterization of AKR1B1 as a prostaglandin F synthase at the maternal-conceptus interface in pigs. Biol Reprod 2014;90:99.
27 Johnson GA, Bazer FW, Burghardt RC, et al. Conceptus-uterus interactions in pigs: endometrial gene expression in response to estrogens and interferons from conceptuses. Soc Reprod Fertil Suppl 2009;66:321-32.
28 Seo H, Kim M, Choi Y, Lee CK, Ka H. Analysis of lysophosphatidic acid (LPA) receptor and LPA-induced endometrial prostaglandinendoperoxide synthase 2 expression in the porcine uterus. Endocrinology 2008;149:6166-75.   DOI
29 Seo H, Choi Y, Shim J, Choi Y, Ka H. Regulatory mechanism for expression of IL1B receptors in the uterine endometrium and effects of IL1B on prostaglandin synthetic enzymes during the implantation period in pigs. Biol Reprod 2012;87.
30 Geisert RD, Zavy MT, Wettemann RP, Biggers BG. Length of pseudopregnancy and pattern of uterine protein release as influenced by time and duration of oestrogen administration in the pig. J Reprod Fertil 1987;79:163-72.   DOI