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http://dx.doi.org/10.5713/ajas.16.0317

Effects of purified lignin on in vitro rumen metabolism and growth performance of feedlot cattle  

Wang, Yuxi (Agriculture and Agri-Food Canada, Lethbridge Research and Development Centre)
McAllister, Tim A. (Agriculture and Agri-Food Canada, Lethbridge Research and Development Centre)
Lora, Jairo H. (GreenValue Enterprises LLC)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.30, no.3, 2017 , pp. 392-399 More about this Journal
Abstract
Objective: The objectives were to assess the effects of purified lignin from wheat straw (sodium hydroxide dehydrated lignin; SHDL) on in vitro ruminal fermentation and on the growth performance of feedlot cattle. Methods: In vitro experiments were conducted by incubating a timothy-alfalfa (50:50) forage mixture (48 h) and barley grain (24 h) with 0, 0.25, 0.5, 1.0, and 2.0 mg/mL of rumen fluid (equivalent to 0, 2, 4, 8, and 16 g SHDL/kg diet). Productions of $CH_4$ and total gas, volatile fatty acids, ammonia, dry matter (DM) disappearance (DMD) and digestion of neutral detergent fiber (NDF) or starch were measured. Sixty Hereford-Angus cross weaned steer calves were individually fed a typical barley silage-barley grain based total mixed ration and supplemented with SHDL at 0, 4, 8, and 16 g/kg DM for 70 (growing), 28 (transition), and 121 d (finishing) period. Cattle were slaughtered at the end of the experiment and carcass traits were assessed. Results: With forage, SHDL linearly (p<0.001) reduced 48-h in vitro DMD from 54.9% to 39.2%, NDF disappearance from 34.1% to 18.6% and the acetate: propionate ratio from 2.56 to 2.41, but linearly (p<0.001) increased $CH_4$ production from 9.5 to 12.4 mL/100 mg DMD. With barley grain, SHDL linearly increased (p<0.001) 24-h DMD from74.6% to 84.5%, but linearly (p<0.001) reduced $CH_4$ production from 5.6 to 4.2 mL/100 mg DMD and $NH_3$ accumulation from 9.15 to $4.49{\mu}mol/mL$. Supplementation of SHDL did not affect growth, but tended (p = 0.10) to linearly reduce feed intake, and quadratically increased (p = 0.059) feed efficiency during the finishing period. Addition of SHDL also tended (p = 0.098) to linearly increase the saleable meat yield of the carcass from 52.5% to 55.7%. Conclusion: Purified lignin used as feed additive has potential to improve feed efficiency for finishing feedlot cattle and carcass quality.
Keywords
Feedlot Cattle; Purified Lignin; Rumen Fermentation; Growth Performance; Carcass Quality;
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  • Reference
1 Beauchemin KA, Kreuzer M, O'Mara F, McAllister TA. Nutritional management for enteric methane abatement: a review. Aust J Exp Agric 2008;48:21-7.   DOI
2 Oskov ER, Flatt WP, Moe PW. Fermentation balance approach to estimate extent of fermentation and efficiency of volatile fatty acids formation in ruminants. J Dairy Sci 1968;51:1429.   DOI
3 Goatcher WD, Church D. C. Taste responses in ruminants. 4. Reactions of pygmy goats, normal goats, sheep and cattle to acetic acid and quinine hydrochloride. J Anim Sci 1970;31:373-82.   DOI
4 Valencia Z, Chavez ER. Lignin as a purified dietary fiber supplement for piglets. Nutr Res 1997;17:1517-27.   DOI
5 Baurhoo B, Phillip L, Ruiz-Feria CA. Effects of purified lignin and mannanoligosaccharides on intestinal integrity and microbial populations in the ceca and litter of broiler chickens. Poult Sci 2007;86:1070-8.   DOI
6 Ricke SC, Van der Aar PJ, Fahey Jr GC, Berger L. Influence of dietary fibres on performance and fermentation characteristics of gut contents from growing chicks. Poult Sci 1982;61:1335-43.   DOI
7 Yu B, Tsai CC, Hsu JC, Chiou PW. Effect of different sources of dietary fibre on growth performance, intestinal morphology and caecal carbohydrases of domestic geese. Br Poult Sci 1998;39:560-7.   DOI
8 Dibner JJ, Richards JD. Antibiotic growth promoters in agriculture: history and mode of action. Poult Sci 2005;84:634-43.   DOI
9 Drlica KS, Perlin DS. Antibiotic resistance: understanding and responding to an emerging crisis. Upper Saddle River, NJ: FT Press Science; 2011.
10 Wallace RJ. Antimicrobial properties of plant secondary metabolites. Proc Nutr Soc 2004;63:621-9.   DOI
11 Ogbodo SO, Okeke AC, Ugwuoru CDC, Chukwurah EF. Possible alternatives to reduce antibiotic resistance. Life Sci Med Res 2011; LSMR-24:1-9.
12 Yitbarek MB. Phytogenics as feed additives in poultry production: a review. Int J Ext Res 2015;3:49-60.
13 Zeng Z, Zhang S, Wang H, Piao X. Essential oil and aromatic plants as feed additives in non-ruminant nutrition: a review. J Anim Sci Biotechnol 2015;6:7.   DOI
14 Lora JH, Glasser WG. Recent industrial applications of lignin: a sustainable alternative to nonrenewable materials. J Polym Environ 2002;10:39-48.   DOI
15 Gosselink RJA, de Jong E, Guran B, Abherli A. Co-ordination network for lignin-standardisation, production and applications adapted to market requirements (EUROLIGNIN). Ind Crop Prod 2004;20:121-9.   DOI
16 Transparency Market Research [Internet]. Lignin market - global industry analysis, size, share, growth, trends and forecast, 2015-2023. 2015 [cited 2016 April 15]. Available from: http://www.transparencymarketresearch.com/lignin-market.html.
17 Ugartondo V, Mitjans M, Vinardell MP. Comparative antioxidant and cytotoxic effects of lignins from different sources. Bioresource Technol 2008;99:6683-7.   DOI
18 Dong X, Dong MD, Lu YJ, et al. Antimicrobial and antioxidant activities of lignin from residue of corn stover to ethanol production. Ind Crop Prod 2011;34:1629-34.   DOI
19 Phillip LE, Idziak ES, Kubow S. The potential use of lignin in animal nutrition, and in modifying microbial ecology of the gut. In: Proc 45th East Nutr Conf; 2000 May 13-14; Montreal, Quebec, Canada. Animal Nutrition Association of Canada; 2000. p 165-84.
20 Ayyachamy M, Cliffe FE, Coyne JM, Collier J, Tuohy MG. Lignin: untapped biopolymers in biomass conversion technologies. Biomass Conv Bioref 2013;3:255-67.   DOI
21 Wang Y, Marx T, Lora J, McAllister TA, Phillip LE. Effects of purified lignin on in vitro ruminal fermentation and on growth performance, carcass traits and fecal shedding of Escherichia coli by feedlot lambs. Anim Feed Sci Technol 2009;151:21-31.   DOI
22 Lora JH. Industrial commercial lignins: sources, properties and applications. In: Belgacem MN, Gandinin A, editors. Monomers, Polymers and Composites from Renewable Materials. Oxford, UK: Elsevier; 2009. p. 225-42.
23 Wang Y, Xu Z, Bach SJ, McAllister TA. Effects of phlorotannins from Ascophyllum nodosum (brown seaweed) on ruminal digestion of forage and concentrate diets in vitro. Anim Feed Sci Technol 2008; 145:375-95.   DOI
24 Menke KH, Raab L, Salewski A, et al. The estimation of the digestibility and metabolizable energy content of ruminant feedingstuffs from the gas production when they are incubated with rumen liquor in vitro. J Agric Sci (Cambr) 1979;93:217-22.   DOI
25 Canadian Council on Animal Care (CCAC). Guide to the Care and Use of Experimental Animals. Vol 1E; Olfert D, Cross BM, McWilliam AA, editors; Ottawa, ON; 2009.
26 Fedorak PM, Hrudey SE. A simple apparatus for measuring gas production by methanogenic culture in serum bottles. Environ Technol Lett 1983;4:425-32.   DOI
27 Van Soest PJ, Robertson JB, Lewis BA. Methods for dietary fibre, neutral detergent and non-starch polysaccharides in relation to animal nutrition. J Dairy Sci 1991;74:3583-97.   DOI
28 Kouazounde J, Jin L, Assogba F, et al. Effects of essential oils from medicinal plants acclimated to Benin on in vitro ruminal fermentation of grass. J Sci Food Agric 2015;95:1031-8.   DOI
29 NRC. Nutrient requirements of beef cattle. 7th ed. Washington, DC: Natl Acad Sci Press; 1996.
30 Bailey DRC, Entz T, Fredeen HT, Rahnefeld GW. Operator and machine effects on ultrasonic measurements of beef cows. Livest Prod Sci 1988;18:305-9.   DOI
31 Herrera-Saldana, RE, Huber JT, Poore MH. Dry matter, crude protein and starch degradability of five cereal grains. J Dairy Sci 1990;73:2386-93.   DOI
32 Chaves AV, Thompson LC, Iwaasa AD, et al. Effect of pasture type (alfalfa vs. grass) on methane and carbon dioxide production by yearling beef heifers. Can J Anim Sci 2006;86:409-18.   DOI
33 SAS Institute. SAS User's Guide. Statistics. Cary, NC: SAS Inst, Inc; 2009.
34 Jung HG. Inhibition of structural carbohydrate fermentation by forage phenolics. J Sci Food Agric 1985;36:74-80.   DOI
35 Hartley RD, Akin DE. Effect of forage cell wall phenolic acids and derivatives on rumen microflora. J Sci Food Agric 1989;49:405-11.   DOI
36 Oskoueian E, Abdullah N, Oskoueian A. Effects of flavonoids on rumen fermentation activity, methane production, and microbial population. BioMed Res Int 2013; Article ID 349129.
37 Patra AK, Yu Z. Effects of essential oils on methane production and fermentation by, and abundance and diversity of, rumen microbial populations. Appl Environ Microbiol 2012;78:4271-80.   DOI