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http://dx.doi.org/10.5713/ajas.16.0502

Fermentation quality and in vitro methane production of sorghum silage prepared with cellulase and lactic acid bacteria  

Khota, Waroon (Faculty of Agriculture, Khon Kaen University)
Pholsen, Suradej (Faculty of Agriculture, Khon Kaen University)
Higgs, David (Department of Biological and Environmental Sciences, University of Hertfordshire)
Cai, Yimin (Japan International Research Center for Agricultural Science (JIRCAS))
Publication Information
Asian-Australasian Journal of Animal Sciences / v.30, no.11, 2017 , pp. 1568-1574 More about this Journal
Abstract
Objective: The effects of lactic acid bacteria (LAB) and cellulase enzyme on fermentation quality, microorganism population, chemical composition and in vitro gas production of sorghum silages were studied. Methods: Commercial inoculant Lactobacillus plantarum Chikuso 1 (CH), local selected strain Lactobacillus casei (L. casei) TH 14 and Acremonium cellulase (AC) were used as additives in sorghum silage preparation. Results: Prior to ensiling Sorghum contained $10^4LAB$ and $10^6cfu/g$ fresh matter coliform bacteria. The chemical compositions of sorghum was 26.6% dry matter (DM), 5.2% crude protein (CP), and 69.7% DM for neutral detergent fiber. At 30 days of fermentation after ensiling, the LAB counts increased to a dominant population; the coliform bacteria and molds decreased to below detectable level. All sorghum silages were good quality with a low pH (<3.5) and high lactic acid content (>66.9 g/kg DM). When silage was inoculated with TH14, the pH value was significantly (p<0.05) lower and the CP content significantly (p<0.05) higher compared to control, CH and AC-treatments. The ratio of in vitro methane production to total gas production and DM in TH 14 and TH 14+AC treatments were significantly (p<0.05) reduced compared with other treatments while in vitro dry matter digestibility and gas production did not differ among treatments. Conclusion: The results confirmed that L. casei TH14 could improve sorghum silage fermentation, inhibit protein degradation and decrease methane production.
Keywords
Cellulase; Lactic Acid Bacteria; Methane Production; Sorghum Silage;
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