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http://dx.doi.org/10.5713/ajas.15.0384

Ramie Leaf Extracts Suppresses Adipogenic Differentiation in 3T3-L1 Cells and Pig Preadipocytes  

Lee, Joomin (Department of Food and Nutrition, College of Natural Science, Chosun University)
Kim, Ah-Ra (Department of Food and Nutrition, College of Natural Science, Chosun University)
Lee, Jae-Joon (Department of Food and Nutrition, College of Natural Science, Chosun University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.29, no.9, 2016 , pp. 1338-1344 More about this Journal
Abstract
The present study was carried out to evaluate the anti-obesity effect of different concentrations of extracts of hot air-dried ramie leaf (HR) and freeze-dried ramie leaf (FR) in 3T3-L1 cells and pig preadipocytes. To analyze the effect on cell proliferation, cells were treated with $25{\mu}g/mL$ or $100{\mu}g/mL$ HR or FR extract for 2 days. Cell differentiation was evaluated by measuring glycerol-3-phosphate dehydrogenase and lipoprotein lipase (LPL) activities and intracellular triglyceride content. Treatment with either HR or FR extracts inhibited the proliferation of 3T3-L1 cells and pig preadipocytes in a dose-dependent manner. HR extract treatment inhibited the differentiation of both cell types more effectively than FR treatment. The extent of triglyceride accumulation decreased significantly in both cells following either HR or FR treatment. Furthermore, LPL activity significantly decreased after treatment with HR or FR extract. These results indicated that HR and FR extracts may inhibit proliferation and differentiation of 3T3-L1 cells and pig preadipocytes. Further studies are needed to explore the anti-obesity effect of HR and FR extracts.
Keywords
Ramie Leaf; Preadipocytes; Proliferation; Differentiation; Obesity;
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