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http://dx.doi.org/10.5713/ajas.15.0106

Effect of Microbial and Chemical Combo Additives on Nutritive Value and Fermentation Characteristic of Whole Crop Barley Silage  

Kim, Dong Hyeon (Division of Applied Life Science (BK21 Plus & Institute of Agriculture and Life Science), Gyeongsang National University)
Amanullah, Sardar M. (Division of Applied Life Science (BK21 Plus & Institute of Agriculture and Life Science), Gyeongsang National University)
Lee, Hyuk Jun (Division of Applied Life Science (BK21 Plus & Institute of Agriculture and Life Science), Gyeongsang National University)
Joo, Young Ho (Division of Applied Life Science (BK21 Plus & Institute of Agriculture and Life Science), Gyeongsang National University)
Kim, Sam Churl (Division of Applied Life Science (BK21 Plus & Institute of Agriculture and Life Science), Gyeongsang National University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.28, no.9, 2015 , pp. 1274-1280 More about this Journal
Abstract
This study was conducted to assess the effects of microbial and chemical combo additives on nutritive values, fermentation indices and aerobic stability of whole crop barley silage. Barley forage (Youngyang) was harvested at about 30% dry matter (DM) by treatments, chopped to 5 cm length and treated with distilled water only (CON), Lactobacillus plantarum (INO), propionic acid (PRO) or an equal mixture of INO and PRO (MIX). Barley forages were ensiled in 4 replications for 0, 2, 7, and 100 days. On 100 days of ensiling, MIX silage had higher (p<0.05) in vitro DM digestibility than CON silage, but lower (p<0.05) acid detergent fiber concentration. The pH in all treated silages was lower (p<0.05) than CON silage. The MIX silage had higher (p<0.05) lactate concentration and lactate to acetate ratio than in CON, but lower (p<0.05) yeast count. Aerobic stability in CON, PRO, and MIX silages were higher (p<0.05) than in INO silage. It is concluded that microbial and chemical combo additives using L. plantarum and propionic acid could efficiently improve nutritive values of barley silage in terms of increased in vitro DM digestibility compared to other treatments. In addition, all treatments except CON reduced yeast count which is the initiate microorganism of aerobic spoilage.
Keywords
Aerobic Stability; Barley Silage; Fermentation Indices; Lactobacillus Plantarum; Propionic Acid;
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1 Adesogan, A. T., L. E. Sollenberger, Y. C. Newman, and J. M. B. Vendramin. 2002. Factors affecting forage quality. In: Florida Forage Handbook (Ed. Y. C. Newman). University of Florida IFAS Exension, Gainesville, FL, USA. pp. 179-183.
2 Adesogan, A. T., N. Krueger, M. B. Salawu, D. B. Dean, and C. R. Staples. 2004. The influence of treatment with dual purpose bacterial inoculants or soluble carbohydrates on the fermentation and aerobic stability of bermudagrass. J. Dairy Sci. 87:3407-3416.   DOI   ScienceOn
3 Amanullah, S. M., D. H. Kim, H. J. Lee, Y. H. Joo, S. B. Kim, and S. C. Kim. 2014. Effects of microbial additives on chemical composition and fermentation characteristics of barley silage. Asian Australas. J. Anim. Sci. 27:511-517.   DOI   ScienceOn
4 AOAC. 1995. Official Methods of Aanalysis. 15th edn, Association of Official Analytical Chemists, Arlington, VA, USA.
5 Baah, J., W. Addah, E. K. Okine, and T. A. McAllister. 2011. Effects of homolactic bacterial inoculant alone or combined with an anionic surfactant on fermentation, aerobic stability and in situ ruminal degradability of barley silage. Asian Australas. J. Anim. Sci. 24:369-378.   DOI   ScienceOn
6 Bolsen, K. K., D. R. Bonilla, G. L. Huck, M. A. Young, and R. A. Hart-Thakur. 1996. Effect of a propionic acid bacterial inoculant on fermentation and aerobic stability of whole-plant corn silage. Kansas Agric. Exp. Stn. Res. Rep. 756:77-88.
7 Chaney, A. L. and E. P. Marbach. 1962. Modified reagents for determination of urea and ammonia. Clin. Chem. 8:130-132.
8 Cleale R. M. IV, J. L. Firkins, F. Van Der Beek, J. H. Clark, E. H. Jaster, G. C. McCoy, and T. H. Klusmeyer. 1990. Effect of inoculation of whole plant corn forage with Pediococcus acidilactici and Lactobacillus xylosus on preservation of silage and heifer growth. J. Dairy Sci. 73:711-718.   DOI
9 Courtin, M. G. and S. F. Spoelstra. 1990. A simulation model of the microbiological and chemical changes accompanying the initial stage of aerobic deterioration of silage. Grass Forage Sci. 45:153-165.   DOI
10 Driehuis. F., S. J. W. H. Oude Elferink, and P. G. Van Wikselaar. 2001. Fermentation characteristics and aerobic stability of grass silage inoculated with Lactobacillus buchneri, with or without homofermentative lactic acid bacteria. Grass Forage Sci. 56:330-343.   DOI
11 Filya I. and E. Sucu. 2007. The effect of bacterial inoculants and a chemical preservative on the fermentation and aerobic stability of whole-crop cereal silages. Asian Australas. J. Anim. Sci. 20:378-384.   DOI
12 Hristov, A. N. and T. A. McAllister. 2002. Effect of inoculants on whole-crop barley silage fermentation and dry matter disappearance in situ. J. Anim. Sci. 80:510-516.
13 Hargreaves, A., J. Hill, and J. D. Leaver. 2009. Effect of stage of growth on the chemical composition, nutritive value and ensilability of whole-crop barley. Anim. Feed Sci. Technol. 152:50-61.   DOI   ScienceOn
14 Henderson, N. 1993. Silage additives. Anim. Feed Sci. Technol. 45:35-56.   DOI   ScienceOn
15 Higginbotham G. E., S. C. Mueller, K. K. Bolsen, and E. J. DePeters. 1998. Effects of inoculants containing propionic acid bacteria on fermentation and aerobic stability of corn silage. J. Dairy Sci. 81:2185-2192.   DOI   ScienceOn
16 Kung, L., Jr. and N. K. Ranjit. 2001. The effect of Lactobacillus buchneri and other additives on the fermentation and aerobic stability of barley silage. J. Dairy Sci. 84:1149-1155.   DOI   ScienceOn
17 Kung, L., Jr., J. R. Robinson, N. K. Ranjit, J. H. Chen, C. M. Golt, and J. D. Pesek. 2000. Microbial populations, fermentation end-products, and aerobic stability of corn silage treated with ammonia or a propionic acid-based preservative. J. Dairy Sci. 83:1479-1486.   DOI   ScienceOn
18 Kung, L., Jr., A. C. Sheperd, A. M. Smagala, K. M. Endres, C. A. Bessett, N. K. Ranjit, and J. L. Glancey. 1998. The effect of preservatives based on propionic acid on the fermentation and aerobic stability of corn silage and a total mixed ration. J. Dairy Sci. 81:1322-1330.   DOI   ScienceOn
19 Lindgren, S., K. Petterson, A. Kaspersson, A. Jonsson, and P. Lingvall. 1985. Microbial dynamics during aerobic deterioration of silages. J. Sci. Food Agric. 36:765-774.   DOI
20 McDonald, P., A. R. Henderson, and S. J. E. Heron. 1991. The Biochemistry of Silage. 2nd edn. Chalcombe Publications, Bucks, England.
21 Mills, J. A. and L. Kung, Jr. 2002. The effect of delayed ensiling and application of a propionic acid-based additive on the fermentation of barley silage. J. Dairy Sci. 85:1969-1975.   DOI   ScienceOn
22 Muck, R. E. 1993. The role of silage additives in making high quality silage. In: Silage Production: from Seed to Animal. Proceedings from the National Silage Production Conference, Syracuse, NY, USA. pp. 46-47.
23 Muck, R. E. 2004. Effects of corn silage inoculants on aerobic stability. Trans. ASAE. 47:1011-1016.   DOI
24 Muck, R. E. and L. Kung, Jr. 1997. Effects of silage additives on ensiling. In: Silage: Field to Feedbunk. NRAES-99. Northeast Regional Agricultural Engineering Service, Ithaca, NY, USA. pp. 187-199.
25 Ohyama, Y., S. Masaki, A. Takigawa, and T. Morichi. 1971. Studies on various factors affecting silage fermentation IX: Synergistic effect of inoculation of lactobacillus plantarum and addition of glucose on silage quality. Jap. J. Zootech. Sci. 42:1-8.
26 Pitt, R. E., Y. Liu, and R. E. Muck. 1991. Simulation of the effect of additives on aerobic stability of alfalfa and corn silages. Trans. ASAE. 34:1633-1641.   DOI
27 Ranjit, N. K. and L. Kung, Jr. 2000. The effect of Lactobacillus buchneri, Lactobacillus plantarum, or a chemical preservative on the fermentation and aerobic stability of corn silage. J. Dairy Sci. 83:526-535.   DOI   ScienceOn
28 SAS Institute Inc. (2002). SAS/STAT User's Guide (Version 9). Cary, NC, USA.
29 Tilley, J. M. A. and R. A. Terry. 1963. A two-stage technique for the in vitro digestion of forage crops. Grass Forage Sci. 18:104-111.   DOI
30 Sucu, E. and I. Filya. 2006. Effects of bacterial inoculants on fermentation, aerobic stability and rumen degradability characteristics of wheat silages. Turkish J. Vet. Anim. Sci. 30:187-193.
31 Van Soest, P. J., J. B. Robertson, and B. A. Lewis. 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74:3583-3597.   DOI
32 Weinberg, Z. G. and R. E. Muck. 1996. New trends and opportunities in the development and use of inoculants for silage. FEMS Microbiol. Rev. 19:53-68.
33 Woolford, M. K. 1975. Microbiological screening of the straight chain fatty acids (C1-C12) as potential silage additives. J. Sci. Food Agric. 26:219-228.   DOI
34 Woolford, M. K. 1984. The Silage Fermentation. Marcel Dekker, Inc., New York, NY, USA.
35 Zahiroddini, H., J. Baah, W. Absalom, and T. A. McAllister. 2004. Effect of an inoculant and hydrolytic enzymes on fermentation and nutritive value of whole crop barley silage. Anim. Feed Sci. Technol. 117:317-330.   DOI   ScienceOn
36 Zahiroddini, H., J. Baah, and T. A. McAllister. 2006. Effects of microbial inoculants on the fermentation, nutrient retention, and aerobic stability of barley silage. Asian Australas. J. Anim. Sci. 19:1429-1436.   DOI