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http://dx.doi.org/10.5713/ajas.2014.14046

Recombinant Goat VEGF164 Increases Hair Growth by Painting Process on the Skin of Shaved Mouse  

Bao, Wenlei (College of Life Science, Inner Mongolia University)
Yin, Jianxin (College of Life Science, Inner Mongolia University)
Liang, Yan (College of Life Science, Inner Mongolia University)
Guo, Zhixin (College of Life Science, Inner Mongolia University)
Wang, Yanfeng (College of Life Science, Inner Mongolia University)
Liu, Dongjun (College of Life Science, Inner Mongolia University)
Wang, Xiao (College of Life Science, Inner Mongolia University)
Wang, Zhigang (College of Life Science, Inner Mongolia University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.27, no.9, 2014 , pp. 1355-1359 More about this Journal
Abstract
To detect goat vascular endothelial growth factor (VEGF)-mediated regrowth of hair, full-length VEGF164 cDNA was cloned from Inner Mongolia cashmere goat (Capra hircus) into the pET-his prokaryotic expression vector, and the recombinant plasmid was transferred into E. coli BL21 cells. The expression of recombinant $6{\times}his-gVEGF164$ protein was induced by 0.5 mM isopropyl thio-${\beta}$-D-galactoside at $32^{\circ}C$. Recombinant goat VEGF164 (rgVEGF164) was purified and identified by western blot using monoclonal anti-his and anti-VEGF antibodies. The rgVEGF164 was smeared onto the dorsal area of a shaved mouse, and we noted that hair regrowth in this area was faster than in the control group. Thus, rgVEGF164 increases hair growth in mice.
Keywords
Goat; Vascular Endothelial Growth Factor (VEGF); Recombinant Protein; Hair Growth;
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