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http://dx.doi.org/10.5713/ajas.2013.13567

Association between Genetic Polymorphism in the Swine Leukocyte Antigen-DRA Gene and Piglet Diarrhea in Three Chinese Pig Breeds  

Yang, Q.L. (College of Animal Science and Technology, Gansu Agricultural University)
Zhao, S.G. (College of Animal Science and Technology, Gansu Agricultural University)
Wang, D.W. (College of Animal Science and Technology, Gansu Agricultural University)
Feng, Y. (College of Animal Science and Technology, Gansu Agricultural University)
Jiang, T.T. (College of Animal Science and Technology, Gansu Agricultural University)
Huang, X.Y. (College of Animal Science and Technology, Gansu Agricultural University)
Gun, S.B. (College of Animal Science and Technology, Gansu Agricultural University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.27, no.9, 2014 , pp. 1228-1235 More about this Journal
Abstract
The swine leukocyte antigen (SLA)-DRA locus is noteworthy among other SLA class II loci for its limited variation and has not been investigated in depth. This study was investigated to detect polymorphisms of four exons of SLA-DRA gene and its association with piglet diarrhea in Landrace, Large White and Duroc pigs. No polymorphisms were detected in exon 3, while 2 SNPs (c.178G>A and c.211T>C), 2 SNPs (c.3093A>C and c.3104C>T) and 5 SNPs (c.4167A>G, c.4184A>G, c.4194A>G, c.4246A>G and c.4293G>A) were detected in exon 1, exon 2 and exon 4 respectively, and 1 SNP (c.4081T>C) in intron 3. Statistical results showed that genotype had significant effect on piglet diarrhea, individuals with genotype BC had a higher diarrhea score when compared with the genotypes AA, AB, AC and CC. Futhermore, genotype AC had a higher diarrhea score than the genotype CC in exon 1 (p<0.05); diarrhea scores of genotype AA and BB were higher than those of genotypes AC and CC in exon 2 (p<0.05); individuals with genotype AA had a higher diarrhea score than individuals with genotype AB and BB in exon 4 (p<0.05). Fourteen common haplotypes were founded by haplotype constructing of all SNPs in the three exons, its association with piglet diarrhea appeared that Hap2, 5, 8, 10, and 14 may be the susceptible haplotypes and Hap9 may be the resistant haplotype to piglet diarrhea. The genetic variations identified of the SLA-DRA gene may potentially be functional mutations related to piglet diarrhea.
Keywords
Swine Leukocyte Antigen; DRA Gene; Association; Genetic Polymorphism; Haplotype; Piglet Diarrhea;
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1 Lunney, J. K., C. S. Ho, M. Wysocki, and D. M. Smith. 2009. Molecular genetics of the swine major histocompatibility complex, the SLA complex. Dev. Comp. Immunol. 33:362-374.   DOI   ScienceOn
2 Mallard, B. A., B. N. Wilkie, and B. W. Kennedy. 1989. Genetic and other effects on antibody and cell mediated immune response in swine leucocyte antigen (SLA)-defined miniature pigs. Anim. Genet. 20:167-178.
3 Molina, R. M., S. H. Cha, W. Chittick, S. Lawson, M. P. Murtaugh, E. A. Nelson, J. Christopher-Hennings, K. J. Yoon, R. Evans, R. R. R. Rowland, and J. J. Zimmerman. 2008. Immune response against porcine reproductive and respiratory syndrome virus during acute and chronic infection. Vet. Immunol. Immunopathol. 126:283-292.   DOI   ScienceOn
4 Morris, R. S., P. R. Davies, and D. E. Lawton. 2002. Evolution of diseases in the world's pig industry. In: 17th International Pig Veterinary Society Congress Proceedings, Ames, IA, USA. pp. 1-10.
5 Patch, J. R., L. E. Pedersen, F. N. Toka, M. Moraes, M. J. Grubman, M. Nielsen, G. Jungersen, B. Soren, and W. T. Golde. 2011. Induction of foot-and-mouth disease virus-specific cytotoxic T cell killing by vaccination. Clin. Vaccine Immunol. 18:280-288.   DOI
6 Renard, C. and M. Vaiman. 1989. Possible relationships between SLA and porcine reproduction. Reprod. Nutr. Dev. 29:569-576.   DOI
7 Sachs, D. H., S. Germana, M. Ei-Gamil, K. Gustafsson, F. Hirsch, and K. Pratt. 1988. Class II genes of miniature swine. Immunogeneties 28:22-29.   DOI
8 Sambrook, J. and D. W. Russell. 2001. Molecular Cloning: A Laboratory Manual 3rd ed. Cold Spring Harbor: Cold Spring Harbor Laboratory Press.
9 Chen, F. X., J. Xie, Y. Zhou, N. L. Li, and K. Y. Chou. 2004. Novel SLA-DR alleles of three Chinese pig strains and the related function in human T cell response. Cell. Mol. Immunol. 1:212-218.
10 Essler, S. E., W. Ertl, J. Deutsch, B. C. Ruetgen, S. Groiss, M. Stadler, B. Wysoudil, W. Gerner, C. S. Ho, and A. Saalmueller. 2013. Molecular characterization of swine leukocyte antigen gene diversity in purebred Pietrain pigs. Anim. Genet. 44:202-205.   DOI   ScienceOn
11 Hedrick, P. W. and T. J. Kim. 2000. Genetics of complex polymorphisms: Parasites and maintenance of MHC variation. In: Evolutionary Genetics from Molecules to Morphology (Eds. R. S. Singh and C. B. Krimbas). Cambridge, NY, USA. pp. 204-234.
12 Ho, C. S., J. K. Lunney, A. Ando, C. Rogel-Gaillard, J. H. Lee, L. B. Schook, and D. M. Smith. 2009. Nomenclature for factors of the SLA system, update 2008. Tissue Antigens 73:307-315.   DOI   ScienceOn
13 Ho, C. S., J. K. Lunney, J. H. Lee, M. H. Franzo-Romain, G. W. Martens, R. R. Rowland, and D. M. Smith. 2010b. Molecular characterization of swine leucocyte antigen class II genes in outbred pig populations. Anim. Genet. 41:428-432.
14 Kelly, D. J., J. J. O'Brien, and K. J. McCracken. 1990. Effect of creep feeding on the incidence, duration and severity of post-weaning diarrhea in pigs. Res. Vet. Sci. 49:223-228.
15 Lewis, C. R., T. Ait-Ali, M. Clapperton, A. L. Archibald, and S. Bishop. 2007. Genetic perspectives on host responses to porcine reproductive and respiratory syndrome (PRRS). Viral Immunol. 20:343-358.   DOI   ScienceOn
16 Beuzen, N. D., M. J. Stear, and K. C. Chang. 2000. Molecular markers and their use in animal breeding. Vet. J. 160:42-52.   DOI   ScienceOn
17 Chardon, P., C. Renard, and M. Vaiman. 1999. The major histocompatibility complex in swine. Immunol. Rev. 167:179-192.   DOI   ScienceOn
18 Breast Cancer Association Consortium. 2006. Commonly studied single-nucleotide polymorphisms and breast cancer: Results from the breast cancer association consortium. J. Natl. Cancer Inst. 98:1382-1396.   DOI   ScienceOn
19 Botstein, D., R. L. White, M. Skolnick, and R. W. Davis. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am. J. Hum. Genet. 32:314-331.
20 Byun, S. O., Q. Fang, H. Zhou, and J. G. H. Hickford. 2009. An effective method for silver-staining DNA in large numbers of polyacrylamide gels. Anal. Biochem. 385:174-175.   DOI   ScienceOn
21 Chardon, P., C. Renard, C. R. Gaillard, and M. Vaiman. 2000. The porcine major histocompatibility complex and related paralogous regions: A review. Genet. Sel. Evol. 32:109-128.   DOI   ScienceOn
22 Tamura, K., D. Peterson, N. Peterson, G. Stecher, M. Nei, and S. Kumar. 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. 28:2731-2739.   DOI   ScienceOn
23 Zhou, H. and J. G. H. Hickford. 2008. Clonal polymerase chain reaction-single-strand conformational polymorphism analysis: An effective approach for identifying cloned sequences. Anal. Biochem. 378:111-112.   DOI   ScienceOn
24 SAS. 1999. SAS User's Guide: Statistics (Version 8.01 Ed.). SAS Institute. Inc., Cary, NC, USA.
25 Shinkai, H., A. Arakawa, M. Tanaka-Matsuda, H. Ide-Okumura, K. Terada, M. Chikyu, T. Kawarasaki, A. Ando, and H. Uenishi. 2012. Genetic variability in swine leukocyte antigen class II and Toll-like receptors affects immune responses to vaccination for bacterial infections in pigs. Comp. Immunol. Microbiol. Infect. Dis. 35:523-532.   DOI   ScienceOn
26 Sommer, S. 2005. The importance of immune gene variability (MHC) in evolutionary ecology and conservation. Front. Zool. 2:16.   DOI
27 Stephens, M. and P. Donnelly. 2003. A comparison of Bayesian methods for haplotype reconstruction from population genotype data. Am. J. Hum. Genet. 73:1162-1169.   DOI   ScienceOn
28 Vaiman, D., D. Mecier, K. Moazami-Goudarzi, A. Eggen, R. Ciampolini, A. Lepingle, R. Velmala, J. Kaukinen, S. L. Varvio, and P. Martin. 1994. A set of 99 cattle microsatellites: characterization, synteny mapping, and polymorphism. Mamm. Genome 5:288-297.   DOI   ScienceOn
29 Wang, Z. and J. Moult. 2001. SNPs, proteins structer, and disease. Hum. Mutat. 17:263-270.   DOI   ScienceOn
30 Yang, Q. L., J. J. Kong, S. G. Zhao, L. X. Liu, D. W. Wang, F. S. Li, T. T. Jiang, and Y. Feng. 2012. Polymorphism of SLA-DRA gene exon 2 and its correlation with piglet diarrhea. Acta Vet. Zootech. Sin. 43:1020-1027. [in Chinese, English abstract]
31 Yang, Q. L., J. J. Kong, D. W. Wang, S. G. Zhao, and S. B. Gun. 2013. Swine leukocyte antigen-DQA gene variation and its association with piglet diarrhea in Large White, Landrace and Duroc. Asian Australas. J. Anim. Sci. 26:1065-1071.   과학기술학회마을   DOI   ScienceOn
32 Luetkemeier, E. S., R. S. Malhi, J. E. Beever, and L. B. Schook. 2009. Diversification of porcine MHC class II genes: evidence for selective advantage. Immunogenetics 61:119-129.   DOI
33 Ho, C. S., G. W. Martens, M. S. Amoss Jr, L. N. Gomez-Raya, C. W. Beattie, and D. M. Smith. 2010a. The SLA alleles and haplotypes present in Sinclair and Hanford swine. Dev. Comp. Immunol. 34:250-257.   DOI   ScienceOn
34 Hudson, R. R. 2001. Linkage Disequilibrium and Recombination. In: Handbook of Statistical Genetics. In: Handbook of Statistical Genetics (Eds. D. J. Balding, M. Bishop, and C. Cannings). John Wiley and Sons, Ltd., Chichester, UK. pp. 309-324.