Browse > Article
http://dx.doi.org/10.5713/ajas.2012.12502

Development of Gut Microbiota in a Mouse Model of Ovalbumin-induced Allergic Diarrhea under Sub-barrier System  

Wang, Juan-Hong (Laboratory of Gastrointestinal Microbiology, Nanjing Agricultural University)
Fan, Song-Wei (Laboratory of Gastrointestinal Microbiology, Nanjing Agricultural University)
Zhu, Wei-Yun (Laboratory of Gastrointestinal Microbiology, Nanjing Agricultural University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.26, no.4, 2013 , pp. 545-551 More about this Journal
Abstract
This study aimed to present a mouse model of ovalbumin (OVA) induced allergic diarrhea under a sub-barrier system and investigate the development of gut microbiota in this model. Male BALB/c mice were systemically sensitized with OVA or sham-sensitized with saline, and followed by oral OVA intubation, leading to OVA-specific acute diarrhea. Compared with sham-sensitized mice, sera OVA-specific IgG1 and total IgE in OVA-sensitized mice were dramatically elevated, and the number of mast cells was greatly increased in the jejunum of the OVA-sensitized mice. Principle component analysis of the DGGE profile showed that samples from group of OVA-sensitized mice and group of sham-sensitized mice were scattered into two different regions. Real-time PCR analysis showed that the number of 16S rRNA gene copies of Lactobacillus in the colon of OVA-sensitized mice decreased significantly, while there was no significant difference in the number of Bifidobacterium and total bacteria. In conclusion, OVA-specific allergic diarrhea was successfully induced under a sub-barrier system, and changes of allergic reactions during induction was coupled with changes in gut microbiota, especially the number of colonic Lactobacillus, but the role of gut microbiota in the development of food allergic reactions needs to be further evaluated.
Keywords
Ovalbumin; BALB/c mice; Allergy; Diarrhea; Gut Microbiota;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Forbes, E. E., K. Groschwitz, J. P. Abonia, E. B. Brandt, E. Cohen, C. Blanchard, R. Ahrens, L. Seidu, A. McKenzie, R. Strait, F. D. Finkelman, P. S. Foster, K. I. Matthaei, M. E. Rothenberg and S. P. Hogan. 2008. IL-9- and mast cell-mediated intestinal permeability predisposes to oral antigen hypersensitivity. J. Exp. Med. 205:897-913.   DOI   ScienceOn
2 Forsythe, P., M. D. Inman and J. Bienenstock. 2007. Oral treatment with live Lactobacillus reuteri inhibits the allergic airway response in mice. Am. J. Respir. Crit. Care Med. 175:561-569.   DOI   ScienceOn
3 Helm, R. M., R. W. Ermel and O. L. Frick. 2003. Nonmurine animal models of food allergy. Environ. Health Perspect. 111:239-244.   DOI
4 Hougee, S., A. J. Vriesema, S. C. Wijering, L. M. Knippels, G. Folkerts, F. P. Nijkamp, J. Knol and J. Garssen. 2010. Oral treatment with probiotics reduces allergic symptoms in ovalbumin-sensitized mice: a bacterial strain comparative study. Int. Arch. Allergy Immunol. 151:107-117.   DOI   ScienceOn
5 Iliev, I. D., M. Tohno, D. Kurosaki, T. Shimosato, F. He, M. Hosoda, T. Saito and H. Kitazawa. 2008. Immunostimulatory oligodeoxynucleotide containing TTTCGTTT motif from Lactobacillus rhamnosus GG dna potentially suppresses OVA-specific IgE production in mice. Scand. J. Immunol. 67:370-376.   DOI   ScienceOn
6 Johansson, M. A., Y. M. Sjogren, J. O. Persson, C. Nilsson and E. Sverremark-Ekstrom. 2011. Early colonization with a group of Lactobacilli decreases the risk for allergy at five years of age despite allergic heredity. PLoS One. 6:e23031.   DOI
7 Khafipour, E., S. Li, J. C. Plaizier and D. O. Krause. 2009. Rumen microbiome composition determined using two nutritional models of subacute ruminal acidosis. Appl. Environ. Microbiol. 75:7115-7124.   DOI   ScienceOn
8 Kim, J. Y., Y. O. Choi and G. E. Ji. 2008. Effect of oral probiotics (Bifidobacterium lactis AD011 and Lactobacillus acidophilus AD031) administration on ovalbumin-induced food allergy mouse model. J. Microbiol. Biotechnol. 18:1393-1400.
9 Kweon, M. N., M. Yamamoto, M. Kajiki, I. Takahashi and H. Kiyono. 2000. Systemically derived large intestinal CD4(+) Th2 cells play a central role in STAT6-mediated allergic diarrhea. J. Clin. Invest. 106:199-206.   DOI   ScienceOn
10 Li, F., J. Z. Wu and Y. Li. 2003. Experience of breeding nude mice in laminar flow cabinet. Lab. Anim. Sci. Adm. 20:52-54.
11 Li, H. and W. Chen. 2005. Breeding diabetes scid mice with streamline cabinet. Lab. Anim. Sci. Adm. 22:54-55.
12 Matsuki, T., K. Watanabe, J. Fujimoto, T. Takada and R. Tanaka. 2004. Use of 16S rRNA gene-targeted group-specific primers for real-time PCR analysis of predominant bacteria in human feces. Appl. Environ. Microbiol. 70:7220-7228.   DOI   ScienceOn
13 Muyzer, G., E. C. de Waal and A. G. Uitterlinden. 1993. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl. Environ. Microbiol. 59:695-700.
14 Nubel, U., B. Engelen, A. Felske, J. Snaidr, A. Wieshuber, R. I. Amann, W. Ludwig and H. Backhaus. 1996. Sequence heterogeneities of genes encoding 16S rRNAs in Paenibacillus polymyxa detected by temperature gradient gel electrophoresis. J. Bacteriol.178:5636-5643.
15 Prioult, G. and C. Nagler-Anderson. 2005. Mucosal immunity and allergic responses: lack of regulation and/or lack of microbial stimulation. Immunol. Rev. 206:204-218.   DOI   ScienceOn
16 Sanguinetti, C. J., D. Dias Neto and A. J. Simpson. 1994. Rapid silver staining and recovery of PCR products separated on polyacrylamide gels. Biotechniques 17:914-921.
17 Schouten, B., B. C. van Esch, G. A. Hofman, S. A. van Doorn, J. Knol, A. J. Nauta, J. Garssen, L. E. Willemsen and L. M. Knippels. 2009. Cow milk allergy symptoms are reduced in mice fed dietary synbiotics during oral sensitization with whey. J. Nutr. 139:1398-1403.   DOI   ScienceOn
18 Strachan, D. P. 1989. Hay fever, hygiene, and household size. BMJ. 299:1295-1260.   DOI
19 Sicherer, S. H. and H. A. Sampson. 2006. Food allergy. J. Allergy Clin. Immunol. 117:S470-S475.   DOI   ScienceOn
20 Sjogren, Y. M., M. C. Jenmalmw, M. F. Bottcher, B. Bjorksten and E. Sverremark-Ekstrom. 2009. Altered early infant gut microbiota in children developing allergy up to 5 years of age. Clin. Exp. Allergy 39:518-526.   DOI   ScienceOn
21 Sunada, Y., S. Nakamura and C. Kamei. 2007. Effects of Lactobacillus acidophilus strain L-55 on experimental allergic rhinitis in BALB/c mice. Biol. Pharm. Bull. 30:2163-2166.   DOI   ScienceOn
22 Suzuki, M. T., L. T. Taylor and E. F. DeLong. 2000. Quantitative analysis of small-subunit rRNA genes in mixed microbial populations via 5'-nuclease assays. Appl. Environ. Microbiol. 66:4605-4614.   DOI   ScienceOn
23 Takahashi, N., H. Kitazawa, N. Iwabuchi, J. Z. Xiao, K. Miyaji, K. Iwatsuki and T. Saito. 2006. Immunostimulatory oligodeoxynucleotide from Bifidobacterium Longum suppresses Th2 immune responses in a murine model. Clin. Exp. Immunol. 145:130-138.   DOI   ScienceOn
24 Wang, M., C. Karlsson, C. Olsson, I. Adlerberth, A. E. Wold, D. P. Strachan, P. M. Martricardi, N. Aberg, M. R. Perkin, S. Tripodi, A. R. Coates, B. Hesselmar, R. Saalman, G. Molin and S. Ahrne. 2008. Reduced diversity in the early fecal microbiota of infants with atopic eczema. J. Allergy Clin. Immunol. 121:129-134.   DOI   ScienceOn
25 Won, T. J., B. Kim, Y. T. Lim, D. S. Song, S. Y. Park, E. S. Park, D. I. Lee and K. W. Hwang. 2011. Oral administration of Lactobacillus strains from Kimchi inhibits atopic dermatitis in NC/Nga mice. J. Appl. Microbiol. 110:1195-1202.   DOI   ScienceOn
26 Zoetendal, E. G., A. D. Akkermans and W. M. De Vos. 1998. Temperature gradient gel electrophoresis analysis of 16S rRNA from human fecal samples reveals stable and host-specific communities of active bacteria. Appl. Environ. Microbiol. 64:3854-3859.
27 Bjorksten, B., P. Naaber, E. Sepp and M. Mikelsaar. 1999. The intestinal microflora in allergic Estonian and Swedish two-year-old-children. Clin. Exp. Allergy 29:342-346.   DOI
28 Bashir, M. E., S. Louie, H. N. Shi and C. Nagler-Anderson. 2004. Toll-like receptor 4 signaling by intestinal microbes influences susceptibility to food allergy. J. Immunol. 172:6978-6987.   DOI
29 Beckstead, J. H., P. S. Halverson, C. A. Ries and D. F. Bainton. 1981. Enzyme histochemistry and immunohistochemistry on biopsy specimens of pathologic human bone marrow. Blood. 57:1088-1098.
30 Bischoff, S. C. and S. Kramer. 2007. Human mast cells, bacteria and intestinal immunity. Immunol. Rev. 217:329-337.   DOI   ScienceOn
31 Bjorksten, B., E. Sepp, K. Julge, T. Voor and M. Mikelsaar. 2001. Allergy development and the intestinal microflora during the first year of life. J. Allergy Clin. Immun. 108:516-520.   DOI   ScienceOn
32 Bottcher, M. F., E. K. Nordin, A. Sandin, T. Midtvedt and B. Bjorksten. 2000. Microflora-associated characteristics in faeces from allergic and non-allergic infants. Clin. Exp. Allergy 30:1590-1596.
33 Brandt, E. B., R. T. Strait, D. Hershko, Q. Wang, E. E. Muntel, T. A. Scribner, N. Zimmermann, F. D. Finkelman and M. E. Rothenberg. 2003. Mast cells are required for experimental oral allergen-induced diarrhea. J. Clin. Invest. 2:1666-1677.
34 Brandt, E. B., A. Munitz, T. Orekov, M. K. Mingler, M. McBride, F. D. Finkelman and M. E. Rothenberg. 2009. Targeting IL-4/IL-13 signaling to alleviate oral allergen-induced diarrhea.J. Allergy Clin. Immun. 123:53-58.   DOI   ScienceOn