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http://dx.doi.org/10.5713/ajas.2012.12626

Comparative Analysis of Nkx2-5/GATA4/TBX5 Expression in Chicken, Quail and Chicken-quail Hybrids during the Early Stage of Cardiac Development in Embryos  

Ban, Qian (College of Animal Science and Technology, Shihezi University)
Liu, Xiaojun (College of Animal Science and Technology, Shihezi University)
Hui, Wenqiao (College of Animal Science and Technology, Shihezi University)
Chen, Danying (College of Animal Science and Technology, Shihezi University)
Zhao, Zongsheng (College of Animal Science and Technology, Shihezi University)
Jia, Bin (College of Animal Science and Technology, Shihezi University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.26, no.4, 2013 , pp. 476-482 More about this Journal
Abstract
The present study makes an investigation into expression of genes related to cardiac development in chicken, quail and chicken-quail hybrids during the early stage of embryogenesis. Real-time PCR was used to detect mRNA expressions of Nkx2-5, GATA4 and TBX5 in the heart of chicken, quail and chicken-quail hybrids embryos during the 3rd to 7th days of incubation. Results showed that NKX2-5 mRNA displayed a similar expression trend in chicken, quail and chicken-quail hybrids. The initial and highest expression of Nkx2-5 was focused on the 3rd day of incubation, then it declined till 5th day of incubation, thereafter, it fluctuated. Expression of Nkx2-5 gene in quail was significantly higher than in chicken and chicken-quail hybrids, and no significant difference was observed between the two latter species. GATA4 mRNA showed a similar expression trend between chicken and quail, which displayed a steady increase from 3rd to 6th d, then, the expression level decreased. However, GATA4 mRNA expression in chicken-quail hybrids was significantly higher than that in chicken and quail from 3rd to 5th d (p<0.01), but significantly lower than that in chicken and quail during the later stage of the experiment (p<0.05), due to the dramatic drop from 5th d onwards (p<0.01). TBX5 mRNA expression in chicken and quail showed the same trend as GATA4 expressed in the two species. Furthermore, TBX5 expression in chicken-quail hybrids was significantly higher than that in chicken and quail during the whole course of experiment, although relatively lower TBX5 expression was detected in the early stage. In conclusion, Nkx2-5, GATA4 and TBX5 genes showed dynamic changes during the process of cardiac development in chicken, quail and their hybrids embryos. In addition, the expression trend in chicken was similar to that in quail, and there was no significant difference for gene expression level, except NKX2-5. However, expression of these genes in chicken-quail hybrids was significantly different from their parents, the difference mechanism needs to be further explored.
Keywords
Cardiac Development; Embryogenesis; Gene Expression; Chicken; Quail; Chicken-quail Hybrids;
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1 Cao, T. T. 2010. The study of related genes of causing abnormal sexual differentiation in the chicken and quail intergeneric hybrid. Master Thesis, Shihezi University, Xinjiang, China.
2 Davis, D. L., A. V. Edwards, A. L. Juraszek, A. Phelps, A. Wessels and J. B. E. Burch. 2001. A GATA-6 gene heart-region-specific enhancer provides a novel means to mark and probe a discrete component of the mouse cardiac conduction system. Mech. Develop. 108:105-119.   DOI   ScienceOn
3 Gillio-Meina, C., Y. Y. Hui and H. A. LaVoie. 2003. GATA-4 and GATA-6 transcription factors: expression, immunohistochemical localization, and possible function in the porcine ovary. Biol. Reprod. 68:412-422.
4 Gong, L., G. Qiu, X. Xu and K. Sun. 2006. Advances of heart-specific transcription factors: NKX2-5, TBX5 mad G4TA4 in congenitnl heart disease.Int. J. Genetics. 29:133-136.
5 Gove, C., M. Walmsley, S. Nijjar, D. Bertwistle, M. Guille, G. Partington, A. Bomford and R. Patient. 1997. Over-expression of GATA-6 in Xenopus embryos blocks differentiation of heart precursors. EMBO J. 16:355-368.   DOI   ScienceOn
6 Greenfield, C. L., K. M. Lartin, F. S. Sanders and R. R. Dietert. 1986. Heterochromatin staining pattern of quall-chicken hybrid lymphocytes. J. Hered. 77:216-217.
7 Grepin, C., G. Nemer and M. Nemer. 1997. Enhanced cardiogenesis in embryonic stem cells overexpresssing the GATA-4 transcription factor. Development 24:2387-2395.
8 Huss, D., G. Poynter and R. Lansford. 2008. Japanese quail (Coturnix japonica) as a laboratory animal model. Lab. Anim. 37:513-519.   DOI   ScienceOn
9 Krause, A., W. Zacharias, T. Camarata, B. Linkhart, E. Law, A. Lischk, E. Miljan and H. G. Simon. 2004. Tbx5 and Tbx4 transcription factors interact with a new chicken PDZ-LIM protein in limb and heart development. Dev. Biol. 273:106-120.   DOI   ScienceOn
10 Brand, T., B. Andree, A. Schneider, A. Buchberger and H. H. Arnold. 1997. Chicken NKx2-8, a novel homeobox gene expressed during early heart and foregut development. Mech. Develop. 64:53-59.   DOI   ScienceOn
11 Bruneau, B. G. 2002. Transcriptional regulation of vertebrate cardiac morphogenesis. Circ. Res. 90:509-519.   DOI   ScienceOn
12 Arceci, R. J., A. A. King, M. C. Simon, S. H. Orkin and D. B. Wilson. 1993. Mouse GATA-4: a retinoic acid-inducible GATA-binding transcription factor expressed in endodermally derived tissues and heart. Mol. Cell. Biol. 13:2235-2246.
13 Bisping, E., S. Ikeda, S. W. Kong, O. Tarnavsk, N. Bodyak, J. R. McMullen, S. Rajagopal, J. K. Son, Q. Ma, Z. Springer, P. M. Kang, S. Izumo and W. T. Pu. 2006. Gata-4 is required for maintenance of postnatal cardiac function and protection from pressure overload induced heart failure. Proc. Natl. Acad. Sci. USA. 103:14471-14476.   DOI   ScienceOn
14 Liao, H., X. Guo, L. Zhou, Y. Li, X. Li, D. Liang, D. Li and N. Xu. 2012. Expression of androgen and estrogen receptors in the testicular tissue of chickens, quails and chicken-quail hybrids. Afr. J. Biotechnol. 11:7344-7353.
15 Viragh, S. Z., A. C. Gittenberger-de Groot, R. E. Poelmann and F. Kalman. 1993. Early development of quail heart epicardium and associated vascular and glandular structures. Anat. Embryol (Berl). 188:381-393.
16 Schwartz, R. J. and E. N. Olson. 1999. Building the heart piece by piece: Modularity of cis-elements regulating Nkx2-5 transcription. Development 126:4187-4192.
17 Srivastava, D. and E. N. Olson. 2000. A genetic blueprint for cardiac development. Nature 407:221-226.   DOI   ScienceOn
18 Taksashima, Y. and Y. Mizuma. 1982. The sex ratio of chicken-quail hybrids. Japanese. Poult. Sci. 19:53-55.   DOI
19 Wagner, M. and M. Siddiqui. 2007. Signal transduction in early heart development (II): ventricular chamber specification, trabeculation, and heart valve formation. Exp. Biol. Med. 232:866-880.
20 Warkman, A. S., T. A. Yatskievych, K. M. Hardy, P. A. Krieg and P. B. Antin. 2008. Myocardin expression during avian embryonic heart development requires the endoderm but is independent of BMP signaling. Dev. Dyn. 237:216-221.   DOI   ScienceOn
21 Yang, X., J. Tian, C. Chen, H. Sun, L. Zhong, X. Wu and J. Zhu. 2010. Temporal regulation of p300 on heart-specific transcription factors during mouse cardiogenesis. J. Chongqing Med. Univ. 35:961-965.
22 Minvielle, F. 2007. The future of Japanese quail for research and production. World. Poult. Sci. J. 60:500-507.
23 Liberatore, C. M., R. D. Searcy-Schrick and K. E. Yutzey. 2000. Ventricular expression of TBX5 inhibits normal heart chamber. Development. Dev. Biol. 223:169-180.   DOI   ScienceOn
24 Lucotte, G., A. Perramon and M. Kaminski. 1977. Molecular basis for heterosis in the chicken-quail hybrid. Comp. Biochem. Physiol. B. 56:119-122.
25 MacNeill, C., B. Ayres, A. C. Laverriere and J. B. Burch. 1997. Transcripts for functionally distinct isoforms of chicken GATA-5 are differentially expressed from alternative first exons. J. Biol. Chem. 272:8396-8401.   DOI   ScienceOn
26 Olson, E. N. 2006. Gene regulatory networks in the evolution and development of the heart. Science 313:1922-1927.   DOI   ScienceOn
27 Plageman, T. F. and K. E. Yutzey. 2004. Differential expression and function of Tbx5 and Tbx20 in cardiac Development. J. Biol. Chem. 279:19026-19034.   DOI   ScienceOn
28 Poynter, G., D. Huss and R. Lansford. 2009. Japanese quail: an efficient animal model for the production of transgenic avians. Cold Spring Harb. Protoc. 1:112.
29 Pu, W. T., T. Ishiwata, A. L. Juraszek, Q. Ma and S. Izumo. 2004. GATA4 is a dosage-sensitive regulator of cardiac morphogenesis. Dev. Biol. 275:235-244.   DOI   ScienceOn
30 Saber, A. S., K. M. Shoghy, A. M. Erasha and M. M. Nada. 2008. Cardiac looping and formation of the heart regions in Japanese quail embryo (Coturnix coturnix). J. Vet. Anat. 1:3-13.
31 Searcy, R. D., E. B. Vincent, C. M. Liberatore and K. E. Yutzey. 1998. A GATA-dependent nkx-2.5 regulatory element activates early cardiac gene expression in transgenic mice. Development 125:4461-4470.