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http://dx.doi.org/10.5713/ajas.2011.11390

Effect of Peripheral Administration of Kisspeptin-10 on Dynamic LH Secretion in Prepubertal Ewes  

Wang, Jun (College of Animal Science and Technology, Jilin Agricultural University)
Sun, Lei (College of Animal Husbandry and Veterinary Medicine, Liaoning Medical University)
Zhang, Tao (College of Animal Science and Technology, Jilin Agricultural University)
Zhou, Haizhu (College of Animal Science and Technology, Jilin Agricultural University)
Lou, Yujie (College of Animal Science and Technology, Jilin Agricultural University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.25, no.6, 2012 , pp. 785-788 More about this Journal
Abstract
The aim of the present study was to clarify the effect of kisspeptin-10 on LH secretion in prepubertal ewes. In experiment 1, prepubertal ewes fitted with indwelling jugular catheters were randomly assigned to receive 0, 0.5, 1 or 2 mg of kisspeptin-10 dissolved in saline, and serial blood samples were collected at 15-min intervals for 180 min to analyze the response curves of LH after injection. In experiment 2, prepubertal ewes fitted with indwelling jugular catheters were injected with 0 or 1 mg of kisspeptin-10 dissolved in saline and the injection was repeated 3 times at 1 h interval and serial blood samples were collected at 15-min intervals for 210 min to analyze the response curves of LH after injection. The results showed that single intravenous administration of 0.5, 1 and 2 mg of kisspeptin-10 all could significantly increased LH secretion in prepubertal ewes, and the effect of 1 and 2 mg of kisspeptin-10 on LH secretion was higher than that of 0.5 mg group. The results also showed that repeated intravenous administration of kisspeptin-10 could effectively increase LH secretion and repeated administration did not influence the effect of kisspeptin-10 on LH secretion in prepubertal ewe. In conclusion, the present study indicated that single or repeated intravenous administration of kisspeptin-10 could effectively increase LH secretion in prepubertal ewes.
Keywords
Kisspeptin-10; LH; Prepubertal Ewes;
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