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http://dx.doi.org/10.5713/ajas.2012.12187

Different Phosphate Transport in the Duodenum and Jejunum of Chicken Response to Dietary Phosphate Adaptation  

Fang, Rejun (College of Animal Science and Technology, Hunan Agricultural University)
Xiang, Zhifeng (College of Animal Science and Technology, Hunan Agricultural University)
Cao, Manhu (College of Animal Science and Technology, Hunan Agricultural University)
He, Jia (College of Animal Science and Technology, Hunan Agricultural University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.25, no.10, 2012 , pp. 1457-1465 More about this Journal
Abstract
Intestinal phosphate (Pi) absorption across the apical membrane of small intestinal epithelial cells is mainly mediated by the type IIb Na-coupled phosphate co-transporter (NaPi-IIb), but its expression and regulation in the chicken remain unclear. In the present study, we investigated the mRNA and protein levels of NaPi-IIb in three regions of chicken small intestine, and related their expression levels to the rate of net phosphate absorption. Our results showed that maximal phosphate absorption occurs in the jejunum, however the highest expression levels of NaPi-IIb mRNA and protein occurs in the duodenum. In response to a low-Pi diet (TP 0.2%), there is an adaptive response restricted to the duodenum, with increased brush border membrane (BBM) Na-Pi transport activity and NaPi-IIb protein and mRNA abundance. However, when switched from a low-(TP 0.2%) to a normal diet (TP 0.6%) for 4 h, there is an increase in BBM NaPi-IIb protein abundance in the jejunum, but no changes in BBM NaPi-IIb mRNA. Therefore, our study indicates that Na-Pi transport activity and NaPi-IIb protein expression are differentially regulated in the duodenum vs the jejunum in the chicken.
Keywords
Chicken; NaPi-IIb; Small Intestine; Duodenum; Jejunum;
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1 Saddoris, K. L., J. C. Fleet and J. S. Radcliffe. 2010. Sodium-dependent phosphate uptake in the jejunum is post-transcriptionally regulated in pigs fed a low-phosphorus diet and is independent of dietary calcium concentration. J. Nutr. 4:731-736.
2 Segawa, H., I. Kaneko, S. Yamanaka, M. Ito, M. Kuwahata, Y. Inoue, S. Kato and K. Miyamoto. 2004. Intestinal Na-Pi cotranspotter adaptation to dietuy Pi content in vitamin D receptor null mice. Am. J. Physiol. Renal Physiol. 287:F39-47.   DOI   ScienceOn
3 Sugiura, S. H., K. Kelsey and R. P. Ferraris. 2007. Molecular and conventional responses of large rainbow trout to dietary phosphorus restriction. J. Comp. Physiol. B. 177:461-472.   DOI
4 Villa-Bellosta, R. and V. Sorribas. 2010. Compensatory regulation of the sodium/phosphate cotransporters NaPi-IIc (SCL34A3) and Pit-2 (SLC20A2) during Pi deprivation and acidosis. Pflugers. Arch. 459:499-508.   DOI
5 Xu, H., L. Bai, J. F. Collins and F. K. Ghishan. 2002. Age-dependent regulation of rat intestinal type IIb sodium-phosphate cotransporter by 1, 25-(OH) 2 vitamin D3. Am. J. Physiol. Cell Physiol. 282:C487-C493.   DOI   ScienceOn
6 Yan, F., R. Angel and C. M. Ashwell. 2007. Characterization of the chicken small intestine type IIb sodium phosphate cotransporter. Poult. Sci. 86:67-76.   DOI
7 Magagnin, S., A. Werner, D. Markovich, V. Sorribas, G. Stange, J. Biber and H. Murer. 1993. Expression cloning of human and rat renal cortex Na/Pi cotransport. Proc. Natl. Acad. Sci. USA. 90:5979-5983.   DOI
8 McHaffie, G. S., C. Graham, B. Kohl, U. Strunck-Warnecke and A. Werner. 2007. The role of an intracellular cysteine stretch in the sorting of the type II Na/phosphate cotransporter. Biochim. Biophys. Acta. 1768:2099-2106.   DOI   ScienceOn
9 Miyamoto, K., S. Tatsumi, T. Sondoda, H. Yamamoto, H. Minami, Y. Taketani and E. Takeda. 1995. Cloning and functional expression of a Na-dependent phosphate cotransporter from human kidney: cDNA cloning and functional expression. Biochem. J. 301:81-85.
10 Murer, H., I. Forster and J. Biber. 2004. The sodium phosphate cotransporter family SLC34. Pflugers. Arch. 447:763-767.   DOI
11 Nakagawa, N. and F. K. Ghishan. 1994. Low phosphate diet upregulates the renal and intestinal sodium-dependent phosphate transporter in vitamin D-resistant hypophosphatemic mice. Proc. Soc. Exp. Biol. Med. 205:162-167.   DOI
12 Palmada, M., M. Dieter, A. Speil, C. Bohmer, A. F. Mack, H. J. Wagner, K. Klingel, R. Kandolf, H. Murer, J. Biber, E. I. Closs and F. Lang. 2004. Regulation of intestinal phosphate cotransporter NaPi IIb by ubiquitin ligase Nedd4-2 and by serum- and glucocorticoid-dependent kinase 1. Am. J. Physiol. Gastrointest. Liver Physiol. 287:G143-G150.   DOI   ScienceOn
13 Quamme, G. A. 1985. Phosphate transport in intestinal brush-border membrane vesicles: effect of pH and dietary phosphate. Am. J. physiol. 249:G168-G176.
14 Hattenhaur, O., M. Traebert, H. Murer and J. Biber. 1999. Regulation of small intestinal Na-Pi type IIb cotransporter by dietary phosphate intake. Am. J. Physiol. Gasreoinrest. Liver Physiol. 277:G756-762.
15 Radanovic, T., C. A. Wagner, H. Murer and J. Biber. 2005. Regulation of intestinal phosphate transport. I. Segmental expression and adaptation to low-P (i) diet of the type IIb Na+-Pi cotransporter in mouse small intestine. Am. J. Physiol. 288:G496-G500.
16 Ghishan, F. K., N. Arab and H. Shibata. 1990. Intestinal phosphate transport in spontaneously hypertensive rats and genetically matched controls. Gastroenterology 99:106-112.
17 Hashimoto, M., D. Wang, T. Kamo, Y. Zhu, T. Tsujiuchi, Y. Konishi, M. Tanaka and H. Sugimura. 2000. Isolation and localization of type IIb Na/Pi cotransporter in the developing rat lung. Am. J. Pathol. 157:21-27.   DOI   ScienceOn
18 Heaney, R.P. and B. E. C. Nordin. 2002. Calcium effects nn phnsphoius absorption:implications for the prevention and co-therapy of osteoporosis. J. Am. Coll. Nutr. 21:239-204.   DOI
19 Hector Giral, Yupanqui Caldas, Eileen Sutherland, Paul Wilson, Sophia Breusegem, Nicholas Barry, Judith Blaine, Tao Jiang, Xiaoxin X. Wang and Moshe Levi. 2009. Regulation of rat intestinal Na-dependent phosphate transporters by dietary phosphate. Am. J. Physiol. Renal Physiol. 297:1466-1475.   DOI   ScienceOn
20 Hilfiker, H., O. Hattenhauer, M. Traebert, I. Forster, H. Murer and J. Biber. 1998. Characterization of a murine type II sodiumphosphate cotransporter expressed in mammalian intestine. Proc. Natl. Acad. Sci. USA. 95:14564-14569.   DOI   ScienceOn
21 Huber, K., C. Walter, B. Schroder and G. Breves. 2002. Phosphate transport in the duodenum and jejunum of goats and its adaptation by dietary phosphate and calcium. Am. J. Physiol. Regul. Integr. Comp. Physiol. 283:R296-R302.
22 Bai, L., J. F. Collins and F. K. Ghishan. 2000. Cloning and characterization of a type III Na-dependent phosphate cotransporter from mouse intestine. Am. J. Physiol. Cell Physiol. 279:C1135-C1143.
23 Hurwitz, S. and A. Bar. 1970. The sites of calcium and phosphate absorption in the chick. Poult. Sci. 49:324-325.   DOI
24 Kiyamova, R., V. Gryshkova, G. Ovcharenko and D. Lituyev et al. 2008. Development of monoclonal antibodis specific for the human sodium-dependent phosphate cotransporter NaPi2b. Hybridoma 27:277-284.   DOI   ScienceOn
25 Arima, K., E. R. Hines, P. R. Kiela, J. B. Drees, J. F. Collins and F. K. Chishan. 2002. Glucocorticoid regulation and glycosylation of mouse intestinal type IIb Na-Pi cotransporter during ontogeny. Am. J. Physiol. Gastrointest. Liver Physiol. 283:G426-G434.
26 Blahos, J. and A. D. Care. 1981. The jejunumis the site of maximal rate of intestinal absorption of phosphate in chicks. Physiol. Bohemoslov. 30:157-159.
27 Borowitz, S. M. and F. K. Ghishan. 1989. Phosphate transport in human jejunal bnrsh border membrane vesicles. Gastroenterology 96:4-10.
28 Borowitz, S. M. and G. S. Granrud. 1992. Ontogency of intestinal phosphate absorption in rabbits. Am. J. Physiol. 262:G847-G853.
29 Cross, H. S., H. Debiec and M. Peterlik. 1990. Mechanism and regulation of intestinal phosphate absorption. Miner. Electrolyte Metab. 16:115-124.
30 Collins, J. F. and F. K. Ghishan. 1994. Molecular cloning, functional expression, tissue distribution, and in situ hybridization of the renal sodium phosphate ($Na^{+}$/Pi) transporter in the control and hypophosphatemic mouse. FASEB. J. 8:862-868.