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http://dx.doi.org/10.5713/ajas.2011.11029

Changes of Immunoglobulins and Lymphocyte Subpopulations in Peripheral Blood from Holstein Calves Challenged with Escherichia coli Lipopolysaccharide  

Kim, M.H. (Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University)
Yun, C.H. (Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University)
Kim, G.R. (Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University)
Ko, J.Y. (Jeonnam Branch, Nonghyupfeed)
Lee, Jung-Joo (Department of Animal Science, Chungbuk National University)
Ha, Jong-K. (Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.24, no.5, 2011 , pp. 696-706 More about this Journal
Abstract
The objective of this study was to characterize serum immunoglobulins and lymphocytes subpopulations in the peripheral blood mononuclear cells (PBMCs) of Holstein calves in response to lipopolysaccharide (LPS) challenge from Escherichia coli. Fourteen calves received subcutaneous injections of E. coli LPS at 10 weeks of age, and six calves were injected with saline as a control. The concentrations of total serum IgG and the relative amount of LPS-specific IgG in calves challenged with LPS were significantly higher (p<0.05) compared to control animals and LPS challenge significantly increased (p<0.05) the percentage of $CD5^+$ and $CD21^+$ T cells in PBMCs. Meanwhile, LPS challenge significantly increased (p<0.05, p<0.01) the percentage of $CD8^+$ and $CD25^+$ T cells in peripheral blood mononuclear cells (PBMC) at 7 and 14 Day-post LPS challenge (DPLC), respectively. The composition of $CD4^+CD25^+$ T cells and $CD8^+CD25^+$ T cells from calves challenged with LPS was also higher (p<0.05 and p = 0.562, respectively) than those of control calves at 14 DPLC. In conclusion, LPS challenge not only induces production of IgG with expression of B-cell immune response related cell surface molecules, but also stimulates activation of T-lymphocytes in PBMC. Our results suggest that LPS challenge in calves is a good model to elucidate cellular immune response against Gram-negative bacterial infections.
Keywords
Calf; T-lymphocytes; Immunoglobulins; Lipopolysaccharide;
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1 Sandbulte, M. R. and J. A. Roth. 2002. T-cell populations responsive to bovine respiratory syncytial virus in seronegative calves. Vet. Immunol. Immunopathol. 84:111-123.   DOI   ScienceOn
2 Shinohara, N. and M. Kern. 1976. Differentiation of lymphoid cells: B cell as a direct target and T cell as a regulator in lipopolysaccharide-enhanced induction of immunoglobulin production. J. Immunol. 116:1607-1612.
3 Tough, D. F., S. Siquan and S. Jonathan. 1997. T cells stimulation in vivo by Lipopolysaccharide (LPS). J. Exp. Med. 185:2089-2094.   DOI
4 Vanden Bush, T. J. and R. F. Rosenbusch. 2003. Characterization of the immune response to Mycoplasma bovis lung infection. Vet. Immunol. Immunopathol. 94:23-33.   DOI   ScienceOn
5 Vogel, S. N., M. L. Hilfiker and M. J. Caulfield. 1983. Endotoxin-induced T lymphocyte proliferation. J. Immunol. 130:1774-1779.
6 Waters, W. R., T. E. Rahner, M. V. Palmer, D. Cheng, B. J. Nonnecke and D. J. Whipple. 2003. Expression of L-selectin (CD62L), CD44, and CD25 on activated bovine T cells. Infect. Immun. 71:317-326.   DOI
7 Goodman, M. G. and W. O. Weigle. 1979. T cell regulation of polyclonal B cell responsiveness. I. Helper effects of T cells. J. Immunol. 122:2548-2553.
8 Jyonouchi, H., R. M. Voss and R. A. Good. 1990. Up-regulation and down-regulation of cell surface and MRNA expression of CD5 antigen by various humoral factors on murine 70z/3 pre-B cell leukemia cell line: IL-4 down-regulates CD5 antigen expression. Cell. Immunol. 130:66-78.   DOI   ScienceOn
9 Kim, M. H., C. H. Yun, J. Y. Ko, J. S. Kang, H. S. Kim, S. J. Kang, W. S. Lee, J. H. Kim and J. K. Ha. 2009. Changes of immunophysiological characteristics in neonatal calves experimentally challenge with mixture of live bacteria and virus. J. Dairy Sci. 92:5534-5543.   DOI   ScienceOn
10 Kinsbergen, M., R. M. Bruckmainer and J. W. Blum. 1994. Metabolic, endocrine and hematological responses to intravenous E. coli endotoxin administration in 1-week-old calves. Zentralbl Veterinarmed A. 41:530-547.   DOI
11 Morrison, D. C. and J. L. Ryan. 1979. Bacterial endotoxins and host immune response. Adv. Immunol. 28:293-450.
12 Nonnecke, B. J., W. R. Waters, M. R. Foote, M. V. Palmer, B. L. Miller, T. E. Johnson, H. B. Perry and M. A. Fowler. 2005. Development of an adult-like cell-mediated immune response in calves after early immunization with Mycobacterium bovis bacillus Calmette- Guerin. J. Dairy Sci. 88:195-210.   DOI   ScienceOn
13 Perkins, K. H., M. J. Vandehaar, R. J. Tempelman and J. L. Burton. 2001. Negative energy balance does not decrease expression of leukocyte adhesion or antigen-presenting molecules in cattle. J. Dairy Sci. 84:421-428.   DOI   ScienceOn
14 Rings, D. M. 1985. Salmonellosis in calves. Vet. Clin. North Am. Food Anim. Pract. 1:529-539.
15 Coutinho, A., E. Gronowicz, G. MOller and H. Lemke. 1976. Polyclonal B cell activators. In Mitogens in Immunology (Ed. J. J. Oppenheim and D. L. Rosenstreich). Academic Press, New York, USA, pp. 173-190.
16 Riollet, C., P. Rainard and B. Poutrel. 2001. Cell subpopulations and cytokine expression in cow milk in response to chronic staphylococcus aureus infection. J. Dairy Sci. 84:1077-1084.   DOI   ScienceOn
17 Andersson, J., G. MOller and O. SjOberg. 1972. Selective induction of DNA synthesis in T and B lymphocytes. Cell. Immunol. 4:381-393.   DOI   ScienceOn
18 Burvenich, C., V. Van Merris, J. Mehrzad, A. Diez-Fraile and L. Duchateau. 2003. Severity of E. coli mastitis is mainly determined by cow factors. Vet. Res. 34:521-564.   DOI   ScienceOn
19 Dailey, M. O. 1998. Expression of T lymphocyte adhesion molecules: Regulation during antigen-induced T-cell activation and differentiation. Crit. Rev. Immunol. 18:153-184.   DOI
20 Deluyker, H. A., P. Rossitto, S. N. Van Oye and J. S. Cullor. 2004. Efficacy of an Escherichia coli J-5 mutant strain bacterin in the protection of calves from endotoxin disease caused by subcutaneous challenge with endotoxins from Escherichia coli. Vaccine 23:709-717.   DOI   ScienceOn
21 Elsasser, T. H., M. Richards, R. Collier and G. F. Hartnell. 1996. Physiological responses to repeated endotoxin challenge are selectively affected by recombinant bovine somatotropin administration to calves. Domest. Anim. Endocrinol. 13:91-103.   DOI   ScienceOn
22 Foot, M. R., B. J. Nonnecke, M. A. Fowler, B. L. Miller, D. C. Beitz and W. R. Waters. 2005. Effects of age and nutrition on expression of CD25, CD44 and L-selectin (CD62L) on T cells from neonatal calves. J. Dairy Sci. 88:2718-2729.   DOI   ScienceOn
23 Acres, S. D. 1985. Enterotoxigenic E. coli infections in newborn calves: A review. J. Dairy Sci. 68:229-256.   DOI
24 Foot, M. R., B. J. Nonnecke, D. C. Beitz and W. R. Waters. 2007. Antigen-specific B-cell responses by neonatal calves after early vaccination. J. Dairy Sci. 90:5208-5207.   DOI   ScienceOn
25 Freudenberg, M. A., M. Thomas, G. Marina, K. Christoph, L. Regine and G. Chris. 2001. Role of lipopolysaccharide susceptibility in the innate immune response to Salmonella typhimurium infection: LPS, a primary target for recognition of Gram-negative bacteria. Microbes Infect. 3:1213-1222.   DOI   ScienceOn