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http://dx.doi.org/10.5713/ajas.2010.90275

Molecular Cloning, Tissue Distribution and Expression of Porcine y+L Amino Acid Transporter-1  

Zhi, Ai-min (College of Animal Science, South China Agricultural University)
Zhou, Xiang-yan (ASIAPAC (DongGuan) Biotecnology Co., Ltd.)
Zuo, Jian-jun (College of Animal Science, South China Agricultural University)
Zou, Shi-geng (College of Animal Science, South China Agricultural University)
Huang, Zhi-yi (College of Animal Science, South China Agricultural University)
Wang, Xiao-lan (College of Animal Science, South China Agricultural University)
Tao, Lin (College of Animal Science, South China Agricultural University)
Feng, Ding-yuan (College of Animal Science, South China Agricultural University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.23, no.2, 2010 , pp. 272-278 More about this Journal
Abstract
In this study, we cloned, sequenced and characterized porcine y+L Amino Acid Transporter-1 (y+LAT1). By screening a translated EST database with the protein sequence of the human $y^{+}$LAT1 and by using rapid amplification of cDNA ends (RACE), the full-length cDNA encoding porcine $y^{+}$LAT1 was isolated from porcine intestine RNA. It was 2,111 bp long, encoding a 511 amino acid trans-membrane glycoprotein composed of 12 transmembrane domains. The predicted amino acid sequence was found to be 91%, 90%, 87% and 87% identical to those of cattle, human, mouse and rat $y^{+}$LAT1 respectively. Real-time RT-PCR results indicated that the small intestine had the highest $y^{+}$LAT1 mRNA abundance and the lung had the lowest $y^{+}$LAT1 mRNA abundance. Baby hamster kidney (BHK) cells transfected with green fluorescent protein (GFP) tagged porcine $y^{+}$LAT1 cDNA indicated that the cellular localization of the gene product in BHK was on the plasma membrane.
Keywords
Cloning; Pig; Amino Acid Transporter; $y^{+}$LAT1; SLC7A7;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 1  (Related Records In Web of Science)
Times Cited By SCOPUS : 0
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