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http://dx.doi.org/10.5713/ajas.2010.10299

Porcine Xenotransplantation to Primates  

Min, T.S. (National Research Foundation of Korea)
Han, H.J. (Bio-therapy Human Resources Center, Department of Veterinary Physiology, College of Veterinary Medicine, Chonnam National University)
Park, S.H. (Bio-therapy Human Resources Center, Department of Veterinary Physiology, College of Veterinary Medicine, Chonnam National University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.23, no.11, 2010 , pp. 1535-1542 More about this Journal
Abstract
Xenotransplantation is a hot topic currently, since the demand for diverse organs is increasing in patients. Among many species, pigs are suitable animals for xenotranplantation as they share many anatomical and physiological characteristics with humans. This review article provides an overview of porcine xenotransplantation and the rejection of pig xenotransplants in primates, and use of genetically modified and cloned pigs in xenotransplantation. It also highlights major target organs in porcine xenotransplantation and virus infection in xenotransplantation.
Keywords
Xenotransplantation; Pig; Rejection; Virus Infection;
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1 Tuso, P. J., D. V. Cramer, C. Yasunaga, C. A. Cosenza, G. D. Wuand L. Makowka. 1993. Removal of natural human xenoantibodies to pig vascular endothelium by perfusion of blood through pig kidneys and livers. Transplantation 55:1375-1378.   DOI
2 van der Windt, D. J., G. J. Echeverri, J. N. Ijzermans and D. K.Cooper. 2008. The choice of anatomical site for islet transplantation. Cell Transplant. 17:1005-1014.   DOI   ScienceOn
3 van der Windt, D. J., R. Bottino, A. Casu, N. Campanile, C.Smetanka, J. He, H. Murase, H. Hara, S. Ball, B. E. Loveland,D. Ayares, F. G. Lakkis, D. K. Cooper and M. Trucco. 2009.Long-term controlled normoglycemia in diabetic non-human primates after transplantation with hCD46 transgenic porcine islets. Am. J. Transplant. 9:2716-2726.   DOI   ScienceOn
4 Vodicka, P., K. Jr. Smetana, B. Dvorankova, T. Emerick, Y. Z. Xu,J. Ourednik, V. Ourednik and J. Motlik. 2005. The miniature pig as an animal model in biomedical research. Ann. NY Acad. Sci. 1049:161-171.   DOI   ScienceOn
5 Weiss, E. H., B. G. Lilienfeld, S. Müller, E. Müller, N. Herbach, B.Kessler, R. Wanke, R. Schwinzer, J. D. Seebach, E. Wolf and G.Brem. 2009. HLA-E/human beta2-microglobulin transgenic pigs: protection against xenogeneic human anti-pig natural killer cell cytotoxicity. Transplantation 87:35-43.   DOI   ScienceOn
6 Rogers, S. A., F. Chen, M. Talcott, H. Liapis and M. R.Hammerman. 2006. Glucose tolerance normalization following transplantation of pig pancreatic primordia into nonimmunosuppressed diabetic ZDF rats. Transpl. Immunol. 16:176-184.   DOI   ScienceOn
7 Rogers, S. A., F. Chen, M. R. Talcott, C. Faulkner, J. M. Thomas,M. Thevis and M. R. 2007. Hammerman. Long-term engraftment following transplantation of pig pancreatic primordia into non-immunosuppressed diabetic rhesus macaques. Xenotransplantation 14:591-602.   DOI   ScienceOn
8 Sachs, D. H. and C. Galli. 2009. Genetic manipulation in pigs. Curr. Opin. Organ Transplant. 14:148-153.   DOI   ScienceOn
9 Shimizu, A. and K. Yamada. 2006. Pathology of renal xenograft rejection in pig to non-human primate transplantation. Clin. Transplant. 20:46-52.   DOI   ScienceOn
10 Shimizu, A., Y. Hisashi, K. Kuwaki, Y. L. Tseng, F. J. Dor, S. L.Houser, S. C. Robson, H. J. Schuurman, D. K. Cooper, D. H.Sachs, K. Yamada and R. B. Colvin. 2008. Thrombotic microangiopathy associated with humoral rejection of cardiac xenografts from alpha1,3-galactosyltransferase gene-knockout pigs in baboons. Am. J. Pathol. 172:1471-1481.   DOI   ScienceOn
11 Smolenski, R. T., M. Forni, M. Maccherini, M. L. Bacci, E. M.Slominska, H. Wang, P. Fornasari, R. Giovannoni, F. Simeone,A. Zannoni, G. Frati, K. Suzuki, M. H. Yacoub and M.Lavitrano. 2007. Reduction of hyperacute rejection and protection of metabolism and function in hearts of human decay accelerating factor (hDAF)-expressing pigs. Cardiovasc. Res. 73:143-152.   DOI   ScienceOn
12 Stiegler, P., V. Stadlbauer, F. Hackl, S. Schaffellner, F. Iberer, J.Greilberger, D. Strunk, S. Zelzer, C. Lackner and K. Tscheliessnigg. 2010. Prevention of oxidative stress in porcine islet isolation. J. Artif. Organs 13:38-47.   DOI
13 Tector, A. J., M. Berho, J. A. Fridell, A. DiCarlo, S. Liu, C.Soderland, J. S. Barkun, P. Metrakos and J. I. Tchervenkov. 2001. Rejection of pig liver xenografts in patients with liver failure: implications for xenotransplantation. Liver Transpl. 7:82-89.   DOI   ScienceOn
14 Pierson, R. N. III. 2009. Antibody-mediated xenograft injury: mechanisms and protective strategies. Transpl. Immunol. 21: 65-69.   DOI   ScienceOn
15 Platt, J. L. 1998. New directions for organ transplantation. Nature 392:11-17.   DOI   ScienceOn
16 Ramirez, P., J. Yelamos, P. Parrilla and R. Chavez. 2001. Hepatic xenotransplantation will benefit from strategies aimed to reduce complement activation. Liver Transpl. 7:562-563.   DOI   ScienceOn
17 Prather, R. S., R. J. Hawley, D. B. Carter, L. Lai and J. L. Greenstein. 2003. Transgenic swine for biomedicine and agriculture. Theriogenology 59:115-123.   DOI   ScienceOn
18 Puga Yung, G., M. K. Schneider and J. D. Seebach. 2009. Immune responses to alpha1,3 galactosyltransferase knockout pigs. Curr. Opin. Organ Transplant. 14:154-160.   DOI   ScienceOn
19 Qv, J., G. Chen, Z. Shen, G. Jiang, J. Ding, W. Gao, and C. Chen.2009. Activation of complement in the discordant heart xenotransplantation of pig-to-monkey model and the impact of intrathymic inoculation of xenogeneic antigen combined with whole-body gamma-radiation. Transplant. Proc. 41:3905-3908.   DOI   ScienceOn
20 Ramirez, P., R. Chavez, M. Majado, V. Munitiz, A. Muñoz, Q.Hernandez, C. G. Palenciano, G. Pino-Chavez, M. Loba, A.Minguela, J. Yelamos, M. R. Gago, A. S. Vizcaino, H. Asensi,M. G. Cayuela, B. Segura, F. Marin, A. Rubio, T. Fuente, R.Robles, F. S. Bueno, T. Sansano, F. Acosta, J. M. Rodriguez, F.Navarro, J. Cabezuelo, E. Cozzi, D. J. White, R. Y. Calne and P.Parrilla. 2000. Life-supporting human complement regulator decay accelerating factor transgenic pig liver xenograft maintains the metabolic function and coagulation in the nonhuman primate for up to 8 days. Transplantation 70:989-998.   DOI   ScienceOn
21 Ravelingien, A., F. Mortier, E. Mortier, I. Kerremans and J. Braeckman. 2004. Proceeding with clinical trials of animal to human organ transplantation: a way out of the dilemma. J. Med. Ethics. 30:92-98.   DOI
22 Lunney, J. K. 2007. Advances in swine biomedical model genomics. Int. J. Biol. Sci. 3:179-184.   DOI
23 Mueller, N. J., C. Livingston, C. Knosalla, R. N. Barth, S.Yamamoto, B. Gollackner, F. J. Dor, L. Buhler, D. H. Sachs, K.Yamada, D. K. Cooper and J. A. Fishman. 2004. Activation of porcine cytomegalovirus, but not porcine lymphotropic herpesvirus, in pig-to-baboon xenotransplantation. J. Infect. Dis. 189:1628-1633.   DOI   ScienceOn
24 Makowa, L., D. V. Cramer, A. Hoffman, M. Breda, L. Sher, G.Eiras-Hreha, P. J. Tuso, C. Yasunaga, C. A. Cosenza and G. D.Wu. 1995. The use of pig liver xenograft for temporary support of a patient with fulminant hepatic failure. Transplantation 59:1654-1659.   DOI
25 Manzini, S., A. Vargiolu, I. M. Stehle, M. L. Bacci, M. G. Cerrito,R. Giovannoni, A. Zannoni, M. R. Bianco, M. Forni, P. Donini,M. Papa, H. J. Lipps and M. Lavitrano. 2006. Genetically modified pigs produced with a nonviral episomal vector. Proc. Natl. Acad. Sci. USA. 103:17672-17677.   DOI   ScienceOn
26 Meije, Y., R. R. Tonjes and J. A. Fishman. 2010. Retroviral restriction factors and infectious risk in xenotransplantation. Am. J. Transplant. 10:1511-1516.   DOI   ScienceOn
27 Pereira-Sampaio, M. A., L. A. Favorito and F. J. Sampaio. 2004.Pig kidney: anatomical relationships between the intrarenal arteries and the kidney collecting system. Applied study for urological research and surgical training. J. Urol. 172:2077- 2081.   DOI   ScienceOn
28 Petersen, B., W. Ramackers, A. Tiede, A. Lucas-Hahn, D.Herrmann, B. Barg-Kues, W. Chuettler, L. Friedrich, R.Schwinzer, M. Winkler and H. Niemann. 2009. Pigs transgenic for human thrombomodulin have elevated production of activated protein C. Xenotransplantation 16:486-495.   DOI   ScienceOn
29 Pierson, R. N. 3rd., A. Dorling, D. Ayares, M. A. Rees, J. D.Seebach, J. A. Fishman, B. J. Hering and D. K. Cooper. 2009. Current status of xenotransplantation and prospects for clinical application. Xenotransplantation 16:263-280.   DOI   ScienceOn
30 Knosalla, C., K. Yazawa, A. Behdad, N. Bodyak, H. Shang, L.Bühler, S. Houser, B. Gollackner, A. Griesemer, I. Schmitt-Knosalla, H. J. Schuurman, M. Awwad, D. H. Sachs, D. K.Cooper, K. Yamada, A. Usheva and S. C. Robson. 2009. Renal and cardiac endothelial heterogeneity impact acute vascular rejection in pig-to-baboon xenotransplantation. Am. J. Transplant. 9:1006-1016.   DOI   ScienceOn
31 Komoda, H., S. Miyagawa, T. Omori, Y. Takahagi, H. Murakami,T. Shigehisa, T. Ito, H. Matsuda and R. Shirakura. 2005.Survival of adult islet grafts from transgenic pigs with Nacetylglucosaminyltransferase-III (GnT-III) in cynomolgus monkeys. Xenotransplantation 12:209-216.   DOI   ScienceOn
32 Lavitrano, M., M. Busnelli, M. G. Cerrito, R. Giovannoni, S. Manzini and A. Vargiolu. 2006. Sperm-mediated gene transfer. Reprod. Fertil. Dev. 18:19-23.   DOI   ScienceOn
33 Lee, H., B. Lee, Y. Kim, N. Paik and H. Rho. 2010.Characterization of transgenic pigs that express human decay accelerating factor and cell membrane-tethered human tissue factor pathway inhibitor. Reprod. Domest. Anim. (in press)
34 Li, L., D. Pang, T. Wang, Z. Li, L. Chen, M. Zhang, N. Song, D.Nie, Z. Chen, L. Lai and H. Ouyang. 2009. Production of a reporter transgenic pig for monitoring Cre recombinase activity. Biochem. Biophys. Res. Commun. 382:232-235.   DOI   ScienceOn
35 Lila, N., C. G. McGregor, S. Carpentier, J. Rancic, G. W. Byrne and A. Carpentier. 2010. Gal knockout pig pericardium: new source of material for heart valve bioprostheses. J. Heart Lung Transplant. 29:538-543.   DOI   ScienceOn
36 Lin, C. C., D. K. Cooper and A. Dorling. 2009. Coagulation dysregulation as a barrier to xenotransplantation in the primate. Transpl. Immunol. 21:75-80.   DOI   ScienceOn
37 Issa, N. C., R. A. Wilkinson, A. Griesemer, D. K. Cooper, K.Yamada, D. H. Sachs and J. A. Fishman. 2008. Absence of replication of porcine endogenous retrovirus and porcine lymphotropic herpesvirus type 1 with prolonged pig cell microchimerism after pig-to-baboon xenotransplantation. J. Virol. 82:12441-12448.   DOI   ScienceOn
38 Lin, C. C., M. Ezzelarab, R. Shapiro, B. Ekser, C. Long, H. Hara,G. Echeverri, C. Torres, H. Watanabe, D. Ayares, A. Dorling and D. K. Cooper. 2010. Recipient tissue factor expression is associated with consumptive coagulopathy in pig-to-primate kidney xenotransplantation. Am. J. Transplant. 10:1556-1568.   DOI   ScienceOn
39 Hughes, H. C. 1986. Swine in cardiovascular research. Lab. Anim. Sci. 36:348-350.
40 Inverardi, L. and R. Pardi. 1994. Early events in cell-mediated recognition of vascularized xenografts: cooperative interactions between selected lymphocyte subsets and natural antibodies. Immunol. Rev. 141:71-93.   DOI   ScienceOn
41 Kamar, N., J. Selves, J. M. Mansuy, L. Ouezzani, J. M. Péron, J.Guitard, O. Cointault, L. Esposito, F. Abravanel, M. Danjoux,D. Durand, J. P. Vinel, J. Izopet and L. Rostaing. 2008. Hepatitis E virus and chronic hepatitis in organ-transplant recipients. N. Engl. J. Med. 358:811-817.   DOI   ScienceOn
42 Kawai, T., D. Andrews, R. B. Colvin, D. H. Sachs and A. B.Cosimi. 2000. Thromboembolic complications after treatment with monoclonal antibody against CD40 ligand. Nat. Med. 6:114.
43 Kirk, A. D. and D. M. Harlan. 2001. Reply: Thromboembolic complications after treatment with monoclonal antibody against CD40 ligand. Nat. Med. 6:114.
44 Khalfoun, B., D. Barrat, H. Watier, M. C. Machet, B. Arbeille-Brassart, J. G. Riess, H. Salmon, Y. Gruel, P. Bardos and Y.Lebranchu. 2000. Development of an ex vivo model of pig kidney perfused with human lymphocytes. Analysis of xenogeneic cellular reactions. Surgery 128:447-457
45 Good, A. H., D. K. Cooper, A. J. Malcolm, R. M. Ippolito, E.Koren, F. A. Neethling, Y. Ye, N. Zuhdi and L. R. Lamontagne. 1992. Identification of carbohydrate structures that bind human antiporcine antibodies: implications for discordant xenografting in humans. Transplant Proc. 24:559-562.
46 Kirkeby, S. and H. B. Mikkelsen. 2008. Distribution of the alphaGal- and the non-alphaGal T-antigens in the pig kidney: potential targets for rejection in pig-to-man xenotransplantation. Immunol Cell Biol. 86:363-371.   DOI   ScienceOn
47 Klymiuk, N., B. Aigner, G. Brem and E. Wolf. 2010. Genetic modification of pigs as organ donors for xenotransplantation. Mol. Reprod. Dev. 77:209-221.
48 Ezzelarab, M., B. Garcia, A. Azimzadeh, H. Sun, C. C. Lin, H.Hara, S. Kelishadi, T. Zhang, Y. J. Lin, H. C. Tai, R. Wagner, J.Thacker, N. Murase, K. McCurry, R. N. Barth, D. Ayares, R. N.Pierson 3rd and D. K. Cooper. 2009. The innate immune response and activation of coagulation in alpha1,3-galactosyltransferase gene-knockout xenograft recipients. Transplantation 87:805-812.   DOI   ScienceOn
49 Hara, H., B. Gridelli, Y. J. Lin, A. Marcos and D. K. Cooper. 2008.Liver xenografts for the treatment of acute liver failure: clinical and experimental experience and remaining immunologic barriers. Liver Transpl. 14:425-434.   DOI   ScienceOn
50 Hering, B. J., M. Wijkstrom, M. L. Graham, M. Hårdstedt, T. C.Aasheim, T. Jie, J. D. Ansite, M. Nakano, J. Cheng, W. Li, K.Moran, U. Christians, C. Finnegan, N. Kirchhof and H. J.Schuurman. 2006. Prolonged diabetes reversal after intraportal xenotransplantation of wild-type porcine islets in immunosuppressed nonhuman primates. Nat. Med. 12:301-303.   DOI   ScienceOn
51 Hisashi, Y., K. Yamada, K. Kuwaki, Y. L. Tseng, F. J. Dor, S. L.Houser, S. C. Robson, H. J. Schuurman, D. K. Cooper, D. H.Sachs, R. B. Colvin and A. Shimizu. 2008. Rejection of cardiac xenografts transplanted from alpha1,3-galactosyltransferase gene-knockout (GalT-KO) pigs to baboons. Am. J. Transplant. 8:2516-2526.   DOI   ScienceOn
52 Diamond, L. E., C. M. Quinn, M. J. Martin, J. Lawson, J. L. Plattand J. S. Logan. 2001. A human CD46 transgenic pig model system for the study of discordant xenotransplantation. Transplantation 71:132-142.   DOI   ScienceOn
53 Hofmann, A., B. Kessler, S. Ewerling, M. Weppert, B. Vogg, H.Ludwig, M. Stojkovic, M. Boelhauve, G. Brem, E. Wolf and A.Pfeifer. 2003. Efficient transgenesis in farm animals by lentiviral vectors. EMBO Rep. 4:1054-1060.   DOI
54 Horvath, K. A., P. C. Corcoran, A. K. Singh, R. F. Hoyt, C. Carrier,M. L. 3rd Thomas and M. M. Mohiuddin. 2010. Left ventricular pressure measurement by telemetry is an effective means to evaluate transplanted heart function in experimental heterotopic cardiac xenotransplantation. Transplant. Proc. 42:2152-2155.   DOI   ScienceOn
55 Denner, J., H. J. Schuurman and C. Patience. 2009. The International Xenotransplantation Association consensus statement on conditions for undertaking clinical trials of porcine islet products in type 1 diabetes-chapter 5: Strategies to prevent transmission of porcine endogenous retroviruses. Xenotransplantation 16:239-248.   DOI   ScienceOn
56 Dieckhoff, B., A. Karlas, A. Hofmann, W. A. Kues, B. Petersen, A.Pfeifer, H. Niemann, R. Kurth and J. Denner. 2007. Inhibition of porcine endogenous retroviruses (PERVs) in primary porcine cells by RNA interference using lentiviral vectors. Arch. Virol. 152:629-634.   DOI
57 Diswall, M., J. Angström, H. Karlsson, C. J. Phelps, D. Ayares, S.Teneberg and M. E. Breimer. 2010. Structural characterization of alpha1,3-galactosyltransferase knockout pig heart and kidney glycolipids and their reactivity with human and baboon antibodies. Xenotransplantation 17:48-60.   DOI   ScienceOn
58 Ekser, B., B. Gridelli, A. J. Tector and D. K. Cooper. 2009. Pig liver xenotransplantation as a bridge to allotransplantation: which patients might benefit? Transplantation 88:1041-1049.   DOI   ScienceOn
59 Ekser, B., P. Rigotti, B. Gridelli and D. K. Cooper. 2009. Xenotransplantation of solid organs in the pig-to-primate model. Transpl. Immunol. 21:87-92.   DOI   ScienceOn
60 Ekser, B., G. J. Echeverri, A. C. Hassett, M. H. Yazer, C. Long, M.Meyer, M. Ezzelarab, C. C. Lin, H. Hara, D. J. van der Windt,E. M. Dons, C. Phelps, D. Ayares, D. K. Cooper and B. Gridelli. 2010. Hepatic function after genetically engineered pig liver transplantation in baboons. Transplantation (in press)
61 Gewartowska, M. and W. L. Olszewski. 2007. Hepatocyte transplantation-biology and application. Ann. Transplant. 12:27-36.
62 Chen, G., H. Sun, H. Yang, D. Kubelik, B. Garcia, Y. Luo, Y.Xiang, A. Qian, L. Copeman, W. Liu, C. J. Cardella, W. Wang,Y. Xiong, W. Wall, D. J. White and R. Zhong. 2006. The role of anti-non-Gal antibodies in the development of acute humoral xenograft rejection of hDAF transgenic porcine kidneys in baboons receiving anti-Gal antibody neutralization therapy. Transplantation 81:273-283.   DOI   ScienceOn
63 Cooper, D. K. and A. Casu. 2009. The International Xenotransplantation Association consensus statement on conditions for undertaking clinical trials of porcine islet products in type 1 diabetes--chapter 4: Pre-clinical efficacy and complication data required to justify a clinical trial. Xenotransplantation 16:229-238.   DOI   ScienceOn
64 Cooper, D. K. and J. J. Teuteberg. 2010. Pig heart xenotransplantation as a bridge to allotransplantation. J. Heart Lung Transplant. 29:838-840.   DOI   ScienceOn
65 Cooper, D. K., A. Keogh, J. Brink, P. A. Corris, W. Klepetko, R. N.Pierson, M. Schmoeckel, R. Shirakura and L. WarnerStevenson L. 2000. Report of the Xenotransplantation Advisory Committee of the International Society for Heart and Lung Transplantation: the present status of xenotransplantation and its potential role in the treatment of end-stage cardiac and pulmonary diseases. J. Heart Lung Transplant. 19:1125-1165.   DOI   ScienceOn
66 d'Apice, A. J. and P. J. Cowan. 2009. Xenotransplantation: the next generation of engineered animals. Transpl Immunol. 21:111-115.   DOI   ScienceOn
67 Cooper, D. K., B. Gollackner and D. H. Sachs. 2002. Will the pig solve the transplantation backlog? Annu. Rev. Med. 53:133.   DOI   ScienceOn
68 Cooper, D. K., E. Koren and R. Oriol. 1993. Genetically engineered pigs. Lancet 342: 682-683.   DOI   ScienceOn
69 Cozzi, E., A. W. Tucker, G. A. Langford, G. Pino-Chavez, L.Wright, M. J. O'Connell, V. J. Young, R. Lancaster, M.McLaughlin, K. Hunt, M. C. Bordin and D. J. White. 1997. Characterization of pigs transgenic for human decayaccelerating factor. Transplantation 64:1383-1392.   DOI   ScienceOn
70 Brunetti, D., I. Lagutina, S. Colleoni, R. Duchi, F. Calabrese, M.Seveso, E. Cozzi, G. Lazzari, F. Lucchini and C. Galli. 2008.Transgene expression of green fluorescent protein and germ line transmission in cloned pigs derived from in vitro transfected adult fibroblasts. Cloning Stem Cells 10:409-419.   DOI   ScienceOn
71 Buhler, L., M. Basker, I. P. Alwayn, C. Goepfert, H. Kitamura, T.Kawai, S. Gojo, T. Kozlowski, E. L. Ierino, M. Awwad, D. H.Sachs, R. Sackstein, S. C. Robson and D. K. Cooper. 2000. Coagulation and thrombotic disorders associated with pig organ and hematopoietic cell transplantation in nonhuman primates. Transplantation 70:1323-1331.   DOI   ScienceOn
72 Byrne, G. W., K. R. McCurry, M. J. Martin, S. M. McClellan, J. L.Platt and J. S. Logan. 1997. Transgenic pigs expressing human CD59 and decay-accelerating factor produce an intrinsic barrier to complement-mediated damage. Transplantation 63:149-155.   DOI   ScienceOn
73 Cabot, R. A., B. Kuhholzer, A. W. Chan, L. Lai, K. W. Park, K. Y.Chong, G. Schatten, C. N. Murphy, L. R. Abeydeera, B. N.Day and R. S. Prather. 2001. Transgenic pigs produced using in vitro matured oocytes infected with a retroviral vector. Anim. Biotechnol. 12:205-214.   DOI   ScienceOn
74 Thomas, F. T., T. Ljung, J. Henretta, K. Pittman and J. Thomas.1995. Reversal of type II (NIDDM) diabetes by pancreas islet transplantation: an emerging new concept in pathophysiology of an enigmatic disease. Transplant. Proc. 27:3167-3169.
75 Cardona, K., G. S. Korbutt, Z. Milas, J. Lyon, J. Cano, W. Jiang, H.Bello-Laborn, B. Hacquoil, E. Strobert, S. Gangappa, C. J.Weber, T. C. Pearson, R. V. Rajotte and C. P. Larsen. 2006. Long-term survival of neonatal porcine islets in nonhuman primates by targeting costimulation pathways. Nat. Med. 12:304-306.   DOI   ScienceOn
76 Chen, G., H. Qian, T. Starzl, H. Sun, B. Garcia, X. Wang, Y. Wise,Y. Liu, Y. Xiang, L. Copeman, W. Liu, A. Jevnikar, W. Wall, D.K. Cooper, N. Murase, Y. Dai, W. Wang, Y. Xiong, D. J. Whiteand R. Zhong. 2005. Acute rejection is associated with antibodies to non-Gal antigens in baboons using Gal-knockout pig kidneys. Nat. Med. 11:1295-1298.   DOI   ScienceOn
77 Bauer, A., J. Postrach, M. Thormann, S. Blanck, C. Faber, B.Wintersperger, S. Michel, J. M. Abicht, F. Christ, C. Schmitz,M. Schmoeckel, B. Reichart and P. Brenner. 2010. First experience with heterotopic thoracic pig-to-baboon cardiac xenotransplantation. Xenotransplantation 17:243-249.   DOI   ScienceOn
78 Whitelaw, C. B., P. A. Radcliffe, W. A. Ritchie, A. Carlisle, F. M.Ellard, R. N. Pena, J. Rowe, A. J. Clark, T. J. King and K. A.Mitrophanous. 2004. Efficient generation of transgenic pigs using equine infectious anaemia virus (EIAV) derived vector. FEBS Lett. 571:233-236.   DOI   ScienceOn
79 Yu, P., P. Zhang, L. Zhang, S. F. Li, J. Q. Cheng, Y. R. Lu, Y. P. Li and H. Bu. 2009. Studies on long-term infection of human cells with porcine endogenous retrovirus. Acta Virol. 53:169-174.   DOI
80 Teotia, S. S., R. C. Walker, J. M. Schirmer, H. D. Tazelaar, M. G.Michaels, J. M. Risdahl, G. W. Byrne, J. S. Logan and C. G.McGregor. 2005. Prevention, detection, and management of early bacterial and fungal infections in a preclinical cardiac xenotransplantation model that achieves prolonged survival. Xenotransplantation 12:127-133.   DOI   ScienceOn