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http://dx.doi.org/10.5713/ajas.2010.10010

Expression of Prolactin Receptor mRNA after Melatonin Manipulated in Cashmere Goats Skin during Cashmere Growth  

Yue, Chunwang (College of Animal Science and Technology, Hebei North University)
Du, Lixin (Institute of Animal Science Chinese Academy of Agricultural Sciences)
Zhang, Wei (College of Animal Science and Technology, China Agricultural University)
Zhu, Xiaoping (College of Animal Science and Technology, China Agricultural University)
Kong, Xianghao (College of Animal Science and Technology, Hebei North University)
Jia, Zhihai (College of Animal Science and Technology, China Agricultural University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.23, no.10, 2010 , pp. 1291-1298 More about this Journal
Abstract
The aim of this research was to investigate the dynamic changes of the level of total prolactin receptor (PRLR) mRNA and the short form prolactin receptor (S-PRLR) mRNA in skin of cashmere goats from the initiation of cashmere fibre growth to active growth. Eighteen half-sib wethers were allocated randomly to two groups. Melatonin implants were used in order to initiate growth of cashmere fibre before the normal time and reduce blood plasma prolactin (PRL) concentration. Real-time reverse transcription quantitative polymerase chain reaction (real-time PCR) was used to determine PRLR mRNA expression levels of skin from June to November. The results showed that, in Chinese Inner Mongolia cashmere goats, there were seasonal variations in expression of total PRLR mRNA in skin with levels decreasing from June to October. Synchronously, the cashmere fibre growth rate gradually increased during this period, but the expression levels of S-PRLR mRNA did not decrease along with seasonal variation from initiation to active growth of cashmere fibre. These results suggest that expression levels of S- PRLR mRNA might be involved in the process of cashmere growth. It was also possible that the change of alternative splicing of PRLR occurred in the skin of cashmere goats from proanagen to anagen.
Keywords
Prolactin Receptor; Real-time RT-PCR; Melatonin; Prolactin; Cashmere Fibre; Cashmere Goats;
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1 Wuliji, T., A. Litherland, A. L. Goetsch, T. Sahlu, R. Puchala, L. J.Dawson and T. Gipson. 2003. Evaluation of melatonin and bromocryptine administration in Spanish goats II. Effects on seasonal cashmere growth, yield and fiber characteristics of does. Small Rumin. Res. 49:41-49.   DOI   ScienceOn
2 Yue, C. W., L. X. Du, X. P. Zhu, X. H. Kong, W. Zhang and Z. H. Jia. 2007. Skin deiodinase profiles after melatonin manipulated in Chinese inner Mongolia cashmere goats. Asian-Aust. J. Anim. Sci. 20:1496-1502.   DOI
3 Zhou, H. M., D. Allain, J. Q. Li, W. G. Zhang and X. C. Yu. 2003. Effects of non-genetic factors on production traits of Inner Mongolia cashmere goats in China. Small Rumin. Res. 47:85-89.   DOI   ScienceOn
4 Puchala, R., S. G. Pierzynowski, T. Wuliji, A. L. Goetsch, S. A. Soto-Navarro and T. Sahlu. 2003. Effects of prolactin administered to a perfused area of the skin of Angora goats. J. Anim. Sci. 81:279-284.
5 Rhind, S. M. and S. R. Mcmillen. 1995. Seasonal patterns of secondary fibre growth, moulting and hair follicle activity in Siberian and Icelandic$\times$Scottish feral goats offered high and liw levels of dietary protein. Small Rumin. Res. 17:69-76.
6 Santiago-Moreno, J., A. Gomez-Brunet, A. Gonzalez-Bulnes, A.Toledano-Diaz, B. Malpaux and A. Lopez-Sebastian. 2005. Differences in reproductive pattern between wild and domestic rams are not associated with inter-specific annual variations in plasma prolactin and melatonin concentrations. Dom. Anim. En. 28:416-429.   DOI   ScienceOn
7 Santiago-Moreno, J., A. Lopez-Sebastian, A. Campoa, A.Gonzalez-Bulnes, R. Picazo and A. Gomez-Brunet. 2004. Effect of constant-release melatonin implants and prolonged exposure to a long day photoperiod on prolactin secretion and hair growth in mouflon (Ovis gmelini musimon). Dom. Anim. En. 26:303-314.   DOI   ScienceOn
8 Sun, O. L., H. P. Wang and W. De. 2003. Detection of prolactin receptor in skin of inner Mongolia alpas cashmere goat by chemiluminscent photography western blotting. Sci. Tech. Engineering 3:55-57. (Chinese)
9 Shin, R. P. C. and H. G. Friesen. 1974: Solubilization and purification of a prolactin receptor from the rabbit mammary gland. J. Biol. Chem. 249:7902-7911.
10 Sobolevaa, T. K., I. Vetharaniama, A. J. Nixona, P. Montenegroa, A. J. Pearsona and J. Sneydc. 2005. Mathematical modelling of prolactin-receptor interaction and the corollary for prolactin receptor gene expression in skin. J. Theor. Biol. 234:289-298.   DOI   ScienceOn
11 Winder, L. M., D. R. Scobie, A. R. Bray and R. Bickerstaffe. 1995. Wool growth rate in vitro is independent of host animal nutrition, season, and the potential mediators of photoperiod, melatonin and prolactin. J. Exp. Zool. 272:446-454.   DOI   ScienceOn
12 Huang, S. Y., H. L. Song, E. C. Lin, W. C. Lee, J. C. Chiang and H.L. Tsou. 2006. Association of polymorphisms in epidermal growth factor, prostaglandin-endoperoxide synthase 2 and prolactin receptor genes with semen quality in duroc boars. Asian-Aust. J. Anim. Sci. 19:793-807.   DOI
13 Ibraheem, M., H. Galbraith, J. Scaife and S. Ewen. 1994. Growth of secondary hair follicles on the cashmere goat in vitro and their response to prolactin and melatonin. J. Anat. 185:135-142.
14 Kloren, W. R. L., B. W. Norton and M. J. Waters. 1993. Fleece growth in Australian cashmere goats. III. The seasonal patterns of cashmere and hair growth, and association with growth hormone, prolactin and thyroxine in blood. Aust. J. Agric. Res. 44:1035-1050.   DOI   ScienceOn
15 Marc, E. F., K. Bela, L. Anna and N. Gyorgy. 2000. Prolactin: Structure, function, and regulation of secretion. Physiol. Rev. 80:1523-1631.
16 Nixon, A. J., C. A. Ford, J. E. Wildermoth, A. J. Craven, M. G.Ashby and A. J. Pearson. 2002. Regulation of prolactin receptor expression in ovine skin in relation to circulating prolactin and wool follicle growth status. J. Endocrinol. 172: 605-614.   DOI   ScienceOn
17 McNeilly, A. S. and P. Andrews. 1974. Purification and characterization of caprine prolactin. J. Endocrinol. 60:359-367.   DOI   ScienceOn
18 Nixon, A. J., V. J. Choy, C. A. Ford and A. J. Pearson. 1998. Prolactin receptors are highly expressed in wool follicle dermal papillae. Proc. NZ. Soc. Anim. Prod. 58:298-302.
19 Nixon, A. J., V. J. Choy, A. L. Parry and A. J. Pearson. 1993. Fiber growth initiation in hair follicles of goats treated with melatonin. J. Exp. Zool. 1:47-56.
20 Omelka, R., M. Martiniakov, D. Pekoviov and M. Bauerov. 2008. Associations between Alu I polymorphism in the prolactin receptor gene and reproductive traits of Slovak large white, white meaty and landrace pigs. Asian-Aust. J. Anim. Sci. 21:484-488   DOI
21 Bignon, C., N. Binart, C. Ormandy, L. A. Schuler, P. A. Kelly andJ. Djiane. 1997. Long and short forms of the ovine prolactin receptor: cDNA cloning and genomic analysis reveal that the two forms arise by different alternative splicing mechanisms in ruminants and in rodents. J. Mol. Endocrinol. 19:109-120.   DOI   ScienceOn
22 Bustin, S. A. 2000. Absolute quantification of mRNA using realtime reverse transcription polymerase chain reaction assays. J. Mol. Endocrinol. 25:169-193.   DOI   ScienceOn
23 Emesih, G. C., G. R. Newton, T. H. Teh and J. H. Zia. 1993. Effects of photoperiod and continuous administration of melatonin on plasma concentrations of prolactin in cashmere goats. Small Rumin. Res. 11:247-256.   DOI   ScienceOn
24 Choy, V. J., A. J. Nixon and A. J. Pearson. 1997. Distribution of prolactin receptor immunoreactivity in ovine skin and changes during the wool follicle growth cycle. J. Endocrinol. 155:265-275.   DOI   ScienceOn
25 Dicks, P., A. J. F. Russel and G. A. Lincoln. 1994. The role of prolactin in the reactivation of hair follicles and the spring moult in goats. J. Endocrinol. 143:441-448.   DOI   ScienceOn
26 Dicks, P., A. J. F., Russel and G. A. Lincoln. 1995. The effect of melatonin implants administered from December until April, on plasma prolactin, triiodothyronine and thyroxine concentrations and on the timing of the spring moult in cashmere goats. Anim. Sci. 60:239-247.   DOI
27 Foitzik, K., K. Krause, A. J. Nixon, C. A. Ford, U. Ohnemus, A. J.Pearson and R. Paus. 2003. Prolactin and its receptor are expressed in murine hair follicle epithelium, show hair cycledependent expression, and induce catagen. Am. J. Pathol. 162: 1611-1621.   DOI   ScienceOn