Browse > Article
http://dx.doi.org/10.5713/ajas.2009.90091

Effect of Nicotinamide on Proliferation, Differentiation, and Energy Metabolism in Bovine Preadipocytes  

Liu, Xiaomu (College of Animal Science and Technology, China Agricultural University)
Fu, Jinlian (College of Animal Science and Technology, China Agricultural University)
Song, Enliang (Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences)
Zang, Kun (Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences)
Wan, Fachun (Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences)
Wu, Naike (Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences)
Wang, Aiguo (College of Animal Science and Technology, China Agricultural University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.22, no.9, 2009 , pp. 1320-1327 More about this Journal
Abstract
This study examined the effects of nicotinamide on proliferation, differentiation, and energy metabolism in a primary culture of bovine adipocytes. After treatment of cells with 100-500 $\mu{M}$ nicotinamide, cell growth was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and cellular lipid content was assessed by Oil Red O staining and a triglyceride (TG) assay. Several factors related to energy metabolism, namely adenosine triphosphatase (ATPase) activity, nitric oxide (NO) content, nitric oxide synthase (NOS) activity, the number of mitochondria and the relative expression of glyceraldehydes-3-phosphate dehydrogenase (GAPDH), peroxisome proliferator-activated receptor-$\gamma$ ($PPAR_{\gamma}$) and inducible NOS (iNOS), were also investigated. Results showed that nicotinamide induced both proliferation and differentiation in bovine preadipocytes. Nicotinamide decreased NO production by inhibiting NOS activity and iNOS mRNA expression, and controlled lipolytic activity by increasing ATPase activity and the number of mitochondria. The present study provides further evidence of the effects of nicotinamide on lipid and energy metabolism, and suggests that nicotinamide may play an important role in the development of bovine adipose tissue in vivo. This emphasizes the importance of investigating bovine adipose tissue to improve our understanding of dairy cow physiology.
Keywords
Nicotinamide; Bovine Preadipocytes; Nitric Oxide; Triglyceride; Lipolysis; Energy Balance;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 2  (Related Records In Web of Science)
Times Cited By SCOPUS : 2
연도 인용수 순위
1 Cetkovic-Cvrlje, M., S. Sandler and D. L. Eizirik. 1993. Nicotinamide and dexamethasone inhibit interleukin-1-induced nitric oxide production by rinm5f cells without decreasing messenger ribonucleic acid expression for nitric oxide synthase. Endocrinology.133(4):1739-1743   DOI   PUBMED   ScienceOn
2 Goff, J. P. and R. L. Horst. 1997. Physiological changes at parturition and their relationship to metabolic disorders. J. Dairy Sci. 80(7):1260-1268   DOI   ScienceOn
3 Hauschildt, S., P. Scheipers, W. G. Bessler and A. Mulsch. 1992. Induction of nitric oxide synthase in l929 cells by tumournecrosis factor alpha is prevented by inhibitors of poly (adpribose) polymerase. Biochem. J. 288( Pt 1):255-260
4 Jeong, Y. H., S. M. Lee, H. Kim, H. Y. Park, D. Yoon, S. J. Moon, A. Hosoda, D. Kim, S. Saeki and M. Kang. 2008. Cloning, expression, and regulation of bovine cellular retinoic acidbinding Protein-II (CRABP-II) during adipogenesis. Asian-Aust. J. Anim. Sci. 21(11):1551-1558
5 Livak, K. J. and T. D. Schmittgen. 2001. Analysis of relative gene expression data using real-time quantitative pcr and the 2(-delta delta c(t)) method. Methods.25(4):402-408   DOI   ScienceOn
6 Oikarinen, A., J. Makela, T. Vuorio and E. Vuorio. 1991. Comparison on collagen gene expression in the developing chick embryo tendon and heart. Tissue and development timedependent action of dexamethasone. Biochim. Biophys. Acta. 1089:40-46   DOI   PUBMED   ScienceOn
7 Otonkoski, T., G. M. Beattie, M. I. Mally, C. Ricordi and A. Hayek. 1993. Nicotinamide is a potent inducer of endocrine differentiation in cultured human fetal pancreatic cells. J. Clin. Invest. 92(3):1459-1466   DOI   ScienceOn
8 Rolland, V., X. L. Liepvre, M. L. Houbiguian, M. Lavau and I. Dugail. 1995b. C/EBP alpha expression in adipose tissue of genetically obese zucker rats. 207:761-767   DOI   ScienceOn
9 Rosen, E. D., P. Sarraf, A. E. Troy, G. Bradwin, K. Moore, D. S. Milstone, B. M. Spiegelman and R. M. Mortensen. 1999. PPAR gamma is required for the differentiation of adipose tissue in vivo and in vitro. Mol. Cell 4(4):611-617   DOI   ScienceOn
10 Yan, H., E. Aziz, G. Shillabeer, A. Wong, D. Shanghavi, A. Kermouni, M. Abdel-Hafez and D. C. Lau. 2002. Nitric oxide promotes differentiation of rat white preadipocytes in culture. J. Lipid Res. 43(12):2123-2129   DOI   ScienceOn
11 Schmittgen, T. D. and K. J. Livak. 2008. Analyzing real-time pcr data by the comparative ct method. Nat. Protoc. 3(6):1101-1108   DOI   PUBMED   ScienceOn
12 Jaster, E. H., D. F. Bell and T. A. Mcpherron. 1983. Nicotinic acid and serum metabolite concentrations of lactating dairy cows fed supplemental niacin. J. Dairy Sci. 66:1039-1045   DOI   ScienceOn
13 Kronfeld, D. S. and F. Raggi. 1964. Nicotinamide coenzyme concentrations in mammary biopsy samples from ketotic cows. Biochem. J. 90(1):219-224
14 Li, F., Z. Z. Chong and K. Maiese. 2006. Cell life versus cell longevity: The mysteries surrounding the nad+ precursor nicotinamide. Curr. Med. Chem. 13(8):883-895   DOI   ScienceOn
15 Lehnert, S. A., A. Reverter, K. A. Byrne, Y. Wang, G. S. Nattrass, N. J. Hudson and P. L. Greenwood. 2007. Gene expression studies of developing bovine longissimus muscle from two different beef cattle breeds. BMC Dev. Biol. 7:95-108   DOI   PUBMED   ScienceOn
16 Yu, B.-L., Z. Shui-Ping, X. Xiao-Zhu and S. Z. Dong. 2007. Effect of niacin on cholesterol efflux in 3T3-L1 cells. Chinese Journal of Arteriosclerosis.15(4):289-292
17 Collins, P. B. and S. Chaykin. 1972. The management of nicotinamide and nicotinic acid in the mouse. J. Biol. Chem. 247(3):778-783
18 Engeli, S., J. Janke, K. Gorzelniak, J. Bohnke, N. Ghose, C. Lindschau, F. C. Luft and A. M. Sharma. 2004. Regulation of the nitric oxide system in human adipose tissue. J. Lipid Res. 45(9):1640-1648   DOI   ScienceOn
19 Lewis, J. E., Y. Shimizu and N. Shimizu. 1982. Nicotinamide inhibits adipocyte differentiation of 3t3-l1 cells. FEBS Lett. 146(1):37-41   DOI   ScienceOn
20 Bai, L., W. J. Pang, Y. J. Yang and G. S. Yang. 2008. Modulation of sirt1 by resveratrol and nicotinamide alters proliferation and differentiation of pig preadipocytes. Mol. Cell. Biochem. 307(1-2):129-140   DOI   PUBMED
21 Sirover, M. 1999. New insights into an old protein: The functional diversity of mammalian glyceraldehyde-3-phosphate dehydrogenase. Biochem. Biophy. Acta. 432:159-184
22 Morrison, R. F. and S. R. Farmer. 2000. Hormonal signaling and transcriptional control of adipocyte differentiation. J. Nutr. 130(12):3116S-3121S
23 Hungate, R. E. 1966. The rumen and its microbes. Academic Press, New York
24 Jaster, E. H. and N. E. Ward. 1990. Supplemental nicotinic acid or nicotinamide for lactating dairy cows. J Dairy Sci. 73(10):2880-2887   DOI   ScienceOn
25 Bossy-Wetzel, E. and S. A. Lipton. 2003. Nitric oxide signaling regulates mitochondrial number and function. Cell Death Differ. 10(7):757-760   DOI   ScienceOn
26 Magni, G., A. Amici, M. Emanuelli, G. Orsomando, N. Raffaelli and S. Ruggieri. 2004. Enzymology of nad+ homeostasis in man. Cell. Mol. Life Sci. 61(1):19-34   DOI   ScienceOn
27 Medina-Gomez, G., S. Gray and A. Vidal-Puig. 2007. Adipogenesis and lipotoxicity: Role of peroxisome proliferator-activated receptor gamma (ppargamma) and ppargammacoactivator-1 (pgc1). Public Health Nutr. 10(10A):1132-1137   DOI   PUBMED   ScienceOn
28 Stanley, S., K. Wynne, B. McGowan and S. Bloom. 2005. Hormonal regulation of food intake. Physiol. Rev. 85(4):1131-1158   DOI   ScienceOn
29 Rolland, V., I. Dugail, X. L. Liepvre and M. Lavau. 1995a. Evidence of increased glyceraldehyde-3-phosphate dehydrogenase and fatty acid synthetase promoteractivities in transiently transfected adipocytes from genetically obese rats. J. Biol. Chem. 270:1102-1106   DOI   ScienceOn
30 Lin, S. J. and L. Guarente. 2003. Nicotinamide adenine dinucleotide, a metabolic regulator of transcription, longevity and disease. Curr. Opin. Cell Biol. 15(2):241-246   DOI   ScienceOn
31 Pires, J. A. and R. R. Grummer. 2007. The use of nicotinic acid to induce sustained low plasma nonesterified fatty acids in feedrestricted holstein cows. J. Dairy Sci. 90(8):3725-3732   DOI   ScienceOn
32 Yang, J., L. K. Klaidman, A. Nalbandian, J. Oliver, M. L. Chang, P. H. Chan and J. D. J. Adams. 2002. The effects of nicotinamide on energy metabolism following transient focal cerebral ischemia in wistar rats. Neurosci. Lett. 333(2):91-94   DOI   ScienceOn
33 Hauner, H. 2005. Secretory factors from human adipose tissue and their functional role. Proc. Nutr. Soc. 64(2):163-169   DOI
34 Choi, K. C., Y. B. Shrestha, S. G. Roh, D. Hishikawa, M. Kuno, H. Tsuzuki, Y. H. Hong and S. Sasaki. 2003. The role of phosphatidylinositol 3-kinase and mitogenic activated protein kinase on the differentiation of ovine preadipocytes. Asian-Aust. J. Anim. Sci. 16(8):1199-1204
35 Ulbrich, S. E., S. Rehfeld, S. Bauersachs, E. Wolf, R. Rottmayer, S. Hiendleder, M. Vermehren, F. Sinowatz, H. H. Meyer and R. Einspanier. 2006. Region-specific expression of nitric oxide synthases in the bovine oviduct during the oestrous cycle and in vitro. J. Endocrinol. 188(2):205-213   DOI   ScienceOn
36 Kaanders, J. H., L. A. Pop, H. A. Marres, I. Bruaset, F. J. van den Hoogen, M. A. Merkx and A. J. van der Kogel. 2002. Arcon: Experience in 215 patients with advanced head-and-neck cancer. Int. J. Radiat. Oncol. Biol. Phys. 52(3):769-778   DOI   ScienceOn
37 Yang, J., S. P. Zhao, J. Li and S. Z. Dong. 2008. Effect of niacin on adipocyte leptin in hypercholesterolemic rabbits. Cardiovasc. Pathol. 17(4):219-225   DOI   ScienceOn
38 Barroso, I., B. Benito, C. Garci-Jimenez, A. Hernandez, M. J. Obregon and P. Santisteban. 1999. Norepinephrine, triiodothyronine and insulin upregulate glyceraldehyde-3-phosphate dehydrogenase mrna during brown adipocyte differentiation. Eur. J. Endocrinol. 141:169-179   DOI   ScienceOn
39 Heuer, C., Y. H. Schukken and P. Dobbelaar. 1999. Postpartum body condition score and results from the first test day milk as predictors of disease, fertility, yield, and culling in commercial dairy herds. J. Dairy Sci. 82(2):295-304   DOI   ScienceOn
40 Liu, Y. M., J. M. Lacorte, N. Viguerie, C. Poitou, V. Pelloux, B. Guy-Grand, C. Coussieu, D. Langin, A. Basdevant and K. Clement. 2003. Adiponectin gene expression in subcutaneous adipose tissue of obese women in response to shortterm very low calorie diet and refeeding. J. Clin. Endocrinol. Metab. 88:5881-5886   DOI   ScienceOn
41 Vaca, P., G. Berna, F. Martin and B. Soria. 2003. Nicotinamide induces both proliferation and differentiation of embryonic stem cells into insulin-producing cells. Transplant. Proc. 35(5):2021-2023   DOI   ScienceOn
42 Pellat-Deceunynck, C., J. Wietzerbin and J. C. Drapier. 1994. Nicotinamide inhibits nitric oxide synthase mrna induction in activated macrophages. Biochem. J. 297( Pt 1):53-58
43 Barak, Y., M. C. Nelson, E. S. Ong, Y. Z. Jones, P. Ruiz-Lozano, K. R. Chien, A. Koder and R. M. Evans. 1999. Ppar gamma is required for placental, cardiac, and adipose tissue development. Mol. Cell 4(4):585-595   DOI   ScienceOn
44 Komatsu, T., F. Itoh, S. Mikawa and K. Hodate. 2003. Gene expression of resistin in adipose tissue and mammary gland of lactating and non-lactating cows. J. Endocrinol. 178(3):R1-5   DOI   ScienceOn
45 Andrade, J., M. Conde, R. Ramirez, J. Monteseirin, J. Conde, F. Sobrino and F. J. Bedoya. 1996. Protection from nicotinamide inhibition of interleukin-1 beta-induced rin cell nitric oxide formation is associated with induction of mnsod enzyme activity. Endocrinology.137(11):4806-4810   DOI   PUBMED   ScienceOn
46 Campbell, J. M., M. R. Murphy, R. A. Christensen and T. R. Overton. 1994. Kinetics of niacin supplements in lactating dairy cows. J. Dairy Sci. 77:566-575   DOI   ScienceOn
47 Catalan, V., J. Gomez-Ambrosi, F. Rotellar, C. Silva, A. Rodriguez, J. Salvador, M. J. Gil, J. A. Cienfuegos and G. Fruhbeck. 2007. Validation of endogenous control genes in human adipose tissue: Relevance to obesity and obesity-associated type 2 diabetes mellitus. Horm. Metab. Res. 39(7):495-500   DOI   ScienceOn
48 Nisoli, E., E. Clementi, C. Paolucci, V. Cozzi, C. Tonello, C. Sciorati, R. Bracale, A. Valerio, M. Francolini, S. Moncada and M. O. Carruba. 2003. Mitochondrial biogenesis in mammals: The role of endogenous nitric oxide. Science.299(5608):896-899   DOI   PUBMED   ScienceOn
49 Beloor, J., H. K. Kang, C. Yun, S. H. Kim and Y. S. Moon. 2009. Low lysine treatment increases adipogenic potential of bovine intramuscular preadipocytes. Asian-Aust. J. Anim. Sci. 22(5):721-726
50 Williams, A. and D. Ramsden. 2005. Nicotinamide: A double edged sword. Parkinsonism Relat. Disord. 11(7):413-420   DOI   ScienceOn
51 Talke, H., K. P. Maier, M. Kersten and W. Gerok. 1973. Effect of nicotinamide on carbohydrate metabolism in the rat liver during starvation. Eur. J. Clin. Invest. 3(6):467-474   DOI   ScienceOn