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http://dx.doi.org/10.5713/ajas.2009.90048

Low Lysine Treatment Increases Adipogenic Potential of Bovine Intramuscular Preadipocytes  

Beloor, Jagadish (Department of Animal Science & Biotechnology, Jinju National University)
Kang, Hye Kyeong (Department of Animal Science & Biotechnology, Jinju National University)
Yun, Cheol-Heui (Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University)
Kim, Sang Hoon (Department of Biology, Kyung Hee University)
Moon, Yang Soo (Department of Animal Science & Biotechnology, Jinju National University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.22, no.5, 2009 , pp. 721-726 More about this Journal
Abstract
The molecular mechanism of adipocyte differentiation has been well documented. However, the effect of specific nutrients such as lysine on adipocyte differentiation is poorly understood especially in ruminant animals. Therefore, the aim of the present study was to elucidate the influence of lysine on adipocyte differentiation and adipogenic genes in cultured bovine preadipocytes. The preadipocytes were treated with different concentrations of lysine (40, 160, 320 mg/L) or troglitazone (10 ${\mu}M$) for 2 days and then subsequently cultured in differentiation medium until day 6. Expression levels of $C/EBP{\alpha}$ were significantly higher (p<0.001) in 40 and 160 mg/L lysine-treated cells compared to 320 mg/L treatment. Though there was an increasing trend in $PPAR{\gamma}$ expression levels with the decreasing lysine concentration, the results were not significant. The preadipocyte factor (pref-1), expression significantly (p<0.001) reduced with decreasing lysine concentration. The Oil red O staining results were better in 40 mg/L treated cells compared to 160 and 320 mg/L lysine treated cells. Our overall results indicate that insufficient supply of lysine increases the adipogenic potential in bovine intramuscular preadipocytes.
Keywords
Adipogenesis; Preadipocytes; Lysine; Bovine;
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1 Adeola, O. and L. G. Young. 1989. Dietary protein-induced changes in porcine muscle respiration, protein synthesis and adipose tissue metabolism. J. Anim. Sci. 67:664-673   DOI   PUBMED   ScienceOn
2 Bluher, M. 2005. Transgenic adipocyte differentiation, animal models for the study of adipose tissue biology. Best Pract. Res. Clin. Endocrinol. Metab. 19:605-623   DOI   PUBMED   ScienceOn
3 Candek-Potokar, M., L. Lefaucheur, B. Zlender and M. Bonneau. 1998. Effect of age and/or weight at slaughter on longissimus dorsi muscle: biochemical traits and sensory quality in pigs. Meat Sci. 48:287-300   DOI   ScienceOn
4 Gondret, F. and B. Lebret. 2002. Feeding intensity and dietary protein level affect adipocyte cellularity and lipogenic capacity of muscle homogenates in growing pigs, without modification of the expression of sterol regulatory element binding protein. J. Anim. Sci. 80:3184-3193
5 Hong, Y. H., Y. Nishimura, D. Hishikawa, H. Tsuzuki, H. Miyahara, C. Gotoh, K. C. Choi, D. D. Feng, C. Chen, H. G. Lee, K. Katoh, S. G. Roh and S. Sasaki. 2005. Acetate and propionate short chain fatty acids stimulate adipogenesis via GPCR43. Endocrinol. 146:5092-5099   DOI   ScienceOn
6 Katsumata, M., M. Matsumoto, S. Kobayashi and Y. Kaji. 2007. Reduced dietary lysine enhances proportion of oxidative fibers in porcine skeletal muscle. Anim. Sci. J. 79:347-353
7 SAS. 1999. SAS/STAT Software for PC. Release 6.11, SAS Institute, Cary, NC, USA
8 Kawada, T., Y. Kamei and E. Sugimoto. 1996. The possibility of active form of vitamins A and D as suppressors on adipocyte development via ligand-dependent transcriptional regulators. Int. J. Obes. Relat. Metab. Disord. 20:52-57
9 Fernyhough, M. E., E. Okine, G. J. Hausman, J. L. Vierck and M. V. Dodson. 2007. Invited review: PPAR-gamma and GLUT-4 expression as differentiation markers for preadipocyte conversion to become an adipocyte. Domest. Anim. Endocrinol. 33:367-378   DOI   ScienceOn
10 Jeong, H. Y., M. S. Lee, H. Kim, Y. H. Park, D. Yoon, S. J. Moon, A. Hosoda, H. D. Kim, S. Saeki and M. J. Kang. 2008. Cloning, expression, and regulation of bovine cellular retinoic acid-binding protein-2 (CRABP-2) during adipogenesis. Asian-Aust. J. Anim. Sci. 21:1551-1558
11 Ritchie, H. D., S. R. Rust, R. A. Merkel and W. G. Bergen. 1993. Getting rid of excess fat is not an easy task. Feedstuffs 13
12 Lee, S. C., H. J. Lee, D. W. Kim and K. I. Han. 2000. Effect of hormones on proliferation of stromal vascular cells from Hanwoo cattle adipose tissue. Asian-Aust. J. Anim. Sci. 13:161-166
13 Spiegelman, B. M. and J. S. Flier. 1996. Flier, Adipogenesis and obesity: rounding out the big picture. Cell 87:377-389   DOI   ScienceOn
14 Wood, J. D., S. N. Brown, G. R. Nute, F. M. Whittington, A. M. Perry, S. P. Johnson and M. Enser. 1996. Effects of breed, feed level and conditioning time on the tenderness of pork. Meat Sci. 44:105-112   DOI   ScienceOn
15 Aso, H., I. Abe, K. Nakajima, T. Ozutsumi, Yamaguchi and Y. Takamori. 1995. A preadipocyte clonal line from bovine intramuscular adipose tissue, Biochem. Biophys. Res. Commun. 213:369-375   DOI   ScienceOn
16 Kokta, T. A., M. V. Dodson, A. Gertler and R. A. Hill. 2004. Intercellular signalling between adipose tissue and muscle tissue. Domest. Anim. Endocrinol. 27:303-331   DOI   ScienceOn
17 Butterwith, S. C. 1994. Molecular events in adipocyte development. Pharmacol. Ther. 61:399-411   DOI   PUBMED   ScienceOn
18 Castell, A.G., R. L. Cliplef, L. M. Poste-Flynn and G. Butler. 1994. Performance, carcass and pork characteristics of castrates and gilts self-fed diets differing in protein content and lysine: Energy ratio. Canadian J. Anim. Sci. 74:519-528   DOI   ScienceOn
19 Kerr, B. J., F. K. McKeith and R. A. Easter. 1995. Effect on performance and carcass characteristics of nursery to finisher pigs fed reduced crude protein, amino acid-supplemented diets, J. Anim. Sci. 73:433-440
20 Ntambi, M. and Y. C. Kim. 2000. Adipocyte differentiation and gene expression, J. Nut. 130:3122-3126
21 Sugihara, H., N. Yonemitsu, S. Miyabara and S. Toda. 1987. Proliferation of unilocular fat cells in primary culture. J. Lipid Res. 28:1038-1045
22 Hausman, D. B. and M. DiGirolamo. 2001. The biology of white adipocyte proliferation. Obes. Rev. 2:239-254   DOI   ScienceOn
23 Tanaka, S., E. Takahashi, T. Matsui and H. Yano. 2001. Zinc promotes adipocyte differentiation in vitro. Asian-Aust. J. Anim. Sci. 14:966-969
24 Fernyhough, M. E., D. L. Helterline, J. L. Vierck, G. J. Hausman, R. A. Hill and M. V. Dodson. 2005. Dedifferentiation of mature adipocytes to form adipofibroblasts: more than just a possibility. Adipocytes 1:17-24   DOI   ScienceOn