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http://dx.doi.org/10.5713/ajas.2009.80290

Effect of Thyroid Hormone on the Gene Expression of Myostatin in Rat Skeletal Muscle  

Ma, Yi (Tianjin Institute of Animal Science and Veterinary)
Chen, Xiaoqiang (Department of Agronomy, Tianjin Agricultural University)
Li, Qing (State Key Laboratory for Agrobiotechnology, China Agricultural University)
An, Xiaorong (State Key Laboratory for Agrobiotechnology, China Agricultural University)
Chen, Yongfu (State Key Laboratory for Agrobiotechnology, China Agricultural University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.22, no.2, 2009 , pp. 275-281 More about this Journal
Abstract
Modification of thyroid hormone levels has a profound effect on skeletal muscle differentiation, predominantly through direct regulation involving thyroid hormone receptors. Nevertheless, little is known about the regulation of myostatin gene expression in skeletal muscle due to altered concentrations of thyroid hormone. Thus, the goal of our study was to find out whether altered thyroid states could change the gene expression of myostatin, the most powerful inhibitor of skeletal muscle development. A hyperthyroid state was induced in rats by daily injections of L-thyroxine 20 mg/100 g body weight for 14 days, while a hypothyroid state was induced in another group of rats by administering methimazole (0.04%) in drinking water for 14 days. After a period of 14 days of L-thyroxine treatment we observed a significant increase of myostatin expression both in mRNA and protein level. However, decreased expression of myostatin mRNA and protein were observed in hypothyroid rats. Furthermore, our studies demonstrated that the upregulation of myostatin gene expression might be responsible for the loss of body weight induced by altered thyroid hormone levels. We concluded that myostatin played a role in a metabolic process in muscle that was regulated by thyroid hormone.
Keywords
Thyroid Hormone; Skeletal Muscle; Hyperthyroidism; Hypothyroidism; Myostatin; Expression;
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