Browse > Article
http://dx.doi.org/10.5713/ajas.2009.80579

Effects of Cordyceps militaris Mycelia on In vitro Rumen Microbial Fermentation  

Yeo, Joon Mo (Department of Animal Science, Korea National Agricultural College, RDA)
Lee, Shin Ja (Division of Applied Life Science (BK21 Program), Graduate School of Gyeongsang National University)
Lee, Sang Min (Division of Applied Life Science (BK21 Program), Graduate School of Gyeongsang National University)
Shin, Sung Hwan (Division of Applied Life Science (BK21 Program), Graduate School of Gyeongsang National University)
Lee, Sung Hoon (Gyeongsangnam-Do Livestock Veterinary Research Institute)
Ha, Jong K. (Department of Agricultural Biotechnology, Seoul National University)
Kim, WanYoung (Department of Animal Science, Korea National Agricultural College, RDA)
Lee, Sung Sill (Division of Applied Life Science (BK21 Program), Graduate School of Gyeongsang National University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.22, no.2, 2009 , pp. 201-205 More about this Journal
Abstract
Effects of Cordyceps militaris mycelia on rumen microbial fermentation were determined by measuring in vitro gas production, cellulose digestion and VFA concentrations. C. militaris mycelia was added to buffered rumen fluid with final concentrations of 0.00, 0.10, 0.15, 0.20, 0.25 and 0.30 g/L and incubation times were for 3, 6, 9, 12, 24, 36, 48 and 72 h. At all incubation times, the gas production showed a quadratic increase with the supplementation of C. militaris mycelia; maximum responses were seen with 0.25 g/L supplementation. However, the gas production was significantly lower for the 0.30 g/L supplementation than for the 0.25 g/L supplementation from 9 h to 72 h incubation. The cellulose filter paper (FP) digestion showed a quadratic increase, as did the gas production except at 3 h incubation. The concentration of total VFA was significantly increased by the supplementation of C. militaris mycelia compared with the control treatment; the highest response was also seen with 0.25 g/L supplementation. This was true for responses in the concentration of acetic and propionic acids. As opposed to other responses, the responses of pH to the supplementation of C. militaris mycelia showed a quadratic decrease from 3 h to 36 h incubation. In conclusion, C. militaris mycelia alter the mixed rumen microbial fermentation with increases in the production of gas and VFA, and cellulose FP digestion.
Keywords
Cordyceps militaris; Rumen Microorganisms; In vitro Gas Production;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 1  (Related Records In Web of Science)
Times Cited By SCOPUS : 1
연도 인용수 순위
1 Calabr$\grave{o}$, S., F. Infascelli, F. Bovera, G. Moniello and V. Piccolo. 2001, In vitro degradability of three forages: fermentation kinetics and gas production of NDF and neutral detergentsoluble fraction of forages, J. Sci. Food Agric. 82:222-229   DOI   ScienceOn
2 Doane, P. H., P. Schofield and A. N. Pell. 1997, Neutral detergent fiber disappearance and gas and volatile fatty acid production during the in vitro fermentation of six forages, J. Anim. Sci.75:3342-3352   DOI   PUBMED   ScienceOn
3 Koh, J. H., H. J. Suh and T. S. Ahn. 2003, Hot-water extract from mycelia of Cordyceps sinensis as a substitute for antibiotic growth promoters, Biotechnol. Lett. 25:585-590   DOI   ScienceOn
4 Li, S. P., F. Q. Yang and K. W. K. Tsim. 2006, Quality control of Cordyceps sinensis, a valued traditional Chinese medicine, J. Pharm. Biomed. Anal41:1571-1584   DOI   ScienceOn
5 Menke, K. H. and H. Steingass. 1988, Estimation of the energetic feed value obtained from chemical analysis and gas production using rumen fluid, Anim. Res. Dev. 28:7-55   DOI
6 Nakamura, K., Y. Yamaguchi, S. Kagota, Y. M. Kwon, K. Shinozuka and M. Kunitomo, 1999, Inhibitory effect of Cordyceps sinensis on spontaneous liver metastasis of Lewis lung carcinoma and B16 melanoma cells in syngeneic mice, Jpn. J. Pharmacol. 79:335-341   DOI
7 Yeon, S. H., J. R. Kim and Y. J. Ahn. 2007, Comparison of growth-inhibiting activities of Cordyceps militaris and Paecilomyces japonica cultured on Bombyx mori pupae towards human gastrointestinal bacteria, J. Sci. Food Agric. 87:54-59   DOI   ScienceOn
8 Yu, K. W., K. M. Kim and H. J. Suh. 2003, Pharmacological activities of stromata of Cordyceps scarabaecola, Phytother Res. 17:244-249   DOI   ScienceOn
9 Yu, R., L. Wang, H. Zhang, C. Zhou and Y. Zhao. 2004b, Isolation, purification and identification of polysaccharides from cultured Cordyceps militaris, Fitoterapia. 75:662-666   DOI   ScienceOn
10 Kneifel, H., W. A. Konig, W. Loeffler and R. Muller. 1977, Ophiocordin, an antifungal antibiotic of Cordyceps ophioglossoides, Archiv. Microbiol. 113:121-130   DOI   ScienceOn
11 Trigg, P., W. E. Gutteridge and J. Williamson. 1971, The effect of cordycepin on malaria parasites, Trans. R. Soc. Trop. Med. Hyg. 65:514-520   DOI   ScienceOn
12 Hungate, R. E. 1966, The rumen bacteria. In: The rumen and its microbes (Ed. R. E. Hungate), Academic Press, New York and London, p. 8
13 Yu, R., L. Song, Y. Shao, W. Bin, L. Wang, H. Shang, Y. Wo, W. Ye and X. Yao. 2004a, Isolation and biological properties of polysaccharide CPS-1 from cultured Cordyceps militaris, Fitoterapia. 75:465-472   DOI   ScienceOn
14 Lee, S. S., C. K. Choi, B. H. Ahn, Y. H. Moon, C. H. Kim and J. K. Ha. 2004, In vitro stimulation of rumen microbial fermentation by a rumen anaerobic fungal culture. Anim. Feed Sci. Technol.115:215-226   DOI   ScienceOn
15 Wallace, R. J. 2004, Antimicrobial properties of plant secondary metabolites, Proc. Nutr. Soc. 63:621-629   DOI
16 Krishnamoorthy, U., H. Steingass and K. H. Menke. 1991. Preliminary observation on the relationship between gas production and microbial protein synthesis in vitro. Arch. Anim. Nutr. 5:521-526   DOI   ScienceOn
17 Ahn, Y. J., S. J. Park, S. G. Lee, S. C. Shin and D. H. Choi. 2000, Cordycepin: selective growth inhibitor derived from liquid culture of Cordyceps militaris against Clostridium spp, J. Agric. Food Chem. 48:2744-2748   DOI   ScienceOn
18 Fedorak, P. M. and S. E. Hrudey. 1983, A simple apparatus for measuring gas production by methanogenic cultures in serum bottles, Environ. Technol. Lett. 4:425-432   DOI   ScienceOn
19 Koh, J. H., K. W. Yu, H. J. Suh, Y. M. Choi and T. S. Ahn 2002, Activation of macrophages and the intestinal immune system by orally administered decoction from cultured mycelium of Cordyceps sinensis, Biosci. Biotechnol. Biochem. 66:407-411   DOI   ScienceOn
20 Li, Zheji, Ganfeng Yi, Jingdong Yin, Peng Sun, Defa Li and Chris Knight. 2008, Effects of organic acids on growth performance, gastrointestinal pH, intestinal microbial populations and immune responses of weaned pigs, Asian-Aust. J. Anim. Sci. 21:252-261
21 Al-Mamun, M., C. Tanaka, Y. Hanai, Y. Tamura and H. Sano. 2007, Effects of plantain (Plantago lanceolata L.) herb and heat exposure on plasma glucose metabolism in sheep, Asian-Aust. J. Anim. Sci. 20:894-899
22 SAS Institute, 1996. SAS User's Guide: Statistics. SAS Institute, Inc., Cary, NC, USA
23 Getachew, G., P. H. Robinson, E. J. DePeters and S. J. Taylor. 2004, Relationships between chemical compositions, dry matter degradation and in vitro gas production of several ruminant feeds, Anim. Feed Sci. Technol. 111:57-71   DOI   ScienceOn