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http://dx.doi.org/10.5713/ajas.2009.80166

Cloning of Chicken Microsomal Glutathione S-transferase 1 Gene (MGST1) and Identification of Its Different Splice Variants  

Wang, X.-T. (State Key Laboratory of Agrobiotechnology and the Key laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University)
Zhang, H. (State Key Laboratory of Agrobiotechnology and the Key laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University)
Zhao, C.-J. (State Key Laboratory of Agrobiotechnology and the Key laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University)
Li, J.-Y. (State Key Laboratory of Agrobiotechnology and the Key laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University)
Xu, G.-Y. (State Key Laboratory of Agrobiotechnology and the Key laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University)
Lian, L.-S. (College of Animal Science and Technology, Yunnan Agricultural University)
Wu, C.-X. (State Key Laboratory of Agrobiotechnology and the Key laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University)
Deng, Xuemei (State Key Laboratory of Agrobiotechnology and the Key laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.22, no.2, 2009 , pp. 155-161 More about this Journal
Abstract
Mammal microsomal glutathione transferase 1 (MGST1) can conjugate many toxic or carcinogenic substances and depress oxidative stress. In this study, Chicken MGST1 and its variants were cloned for the first time and were composed of 956 or 944 nucleotides. The 12 nt deletion in the exon 2 did not alter the GT-AG rule and the ORFs for the two MGST1 variants were the same, which both comprised 465 nucletides and encoded a peptide with 155 amino acids. It was found that the two different splice variants identified using RT-PCR expressed in all three organs investigated of Dwarf Brown Chicken, namely liver, spleen and shell gland. Moreover, the expression level of MGST1 mRNA in the liver of Dwarf Brown chickens was the highest (p<0.01), and there were no significant differences between the spleen and the shell gland. These results provide a base for studying the biological function of Chicken MGST1.
Keywords
Chicken; MGST1; RACE; Splice Variant;
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