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http://dx.doi.org/10.5713/ajas.2009.80185

Effects of ${\beta}$-Mercaptoethanol on the Growth of Preantral Follicles and the Maturation of Intrafollicular Oocytes  

Gong, Seung Pyo (Department of Agricultural Biotechnology, Seoul National University)
Lim, Jeong Mook (Department of Agricultural Biotechnology, Seoul National University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.22, no.1, 2009 , pp. 35-41 More about this Journal
Abstract
This study was undertaken to evaluate how ${\beta}$-mercaptoethanol (bME), an exogenous antioxidant, interacts with preantral follicles cultured in vitro. Mouse primary or secondary follicles were cultured in glutathione (GSH)-free or GSH-containing medium supplemented with bME of various concentrations, and the growth of preantral follicles, the maturation of intrafollicular oocytes and preimplantation development after parthenogenesis were monitored. In experiment 1, 0, 25, 50 or 100 ${\mu}M$ bME was added to culture medium supplemented with 100 ${\mu}M$ GSH or not. When secondary follicles were cultured in GSH-free medium, no significant change in follicle growth was detected after bME addition. However, exposure to bME in the presence of GSH significantly inhibited both follicle growth and oocyte maturation. Such detrimental effect became prominent in primary follicles and bME strongly inhibited follicle growth in the absence of GSH. In conclusion, there are stage-dependent effects of bME on follicle growth and oocyte maturation, and selective use of antioxidants contributes to establishing an efficient follicle culture system.
Keywords
Preantral Follicle; In-vitro Culture; ${\beta}$-Mercaptoethanol; Follicle Growth; Oocyte Maturation;
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1 Almahbobi, G., A. Nagodavithane and A. O. Trounson. 1995. Effects of epidermal growth factor, transforming growth factor alpha and androstenedione on follicular growth and aromatization in culture. Hum. Reprod. 10:2767-2772
2 Caamaño, J. N., Z. Y. Ryoo, J. A. Thomas and C. R. Youngs. 1996. beta-mercaptoethanol enhances blastocyst formation rate of bovine in vitro-matured/in vitro-fertilized embryos. Biol. Reprod. 55:1179-1184   DOI   ScienceOn
3 Demeestere, I., J. Centner, C. Gervy, Y. Englert and A. Delbaere. 2005. Impact of various endocrine and paracrine factors on in vitro culture of preantral follicles in rodents. Reprod. 130:147-156   DOI   PUBMED   ScienceOn
4 Durlinger, A. L., M. J. Gruijters, P. Kramer, B. Karels, T. R. Kumar, M. M. Matzuk, U. M. Rose, F. H. de Jong, J. T. Uilenbroek, J. A. Grootegoed and A. P. Themmen. 2001. Anti-M$\ddot{u}$llerian hormone attenuates the effects of FSH on follicle development in the mouse ovary. Endocrinol. 142:4891-4899   DOI   ScienceOn
5 Lee, S. T., M. H. Choi, E. J. Lee, S. P. Gong, M. Jang, S. H. Park, H. Jee, D. Y. Kim, J. Y. Han and J. M. Lim. 2008. Establishment of autologous embryonic stem cells derived from preantral follicle culture and oocyte parthenogenesis. Fertil. Steril. 90:1910-1920   DOI   ScienceOn
6 Lee, S. T., M. H. Choi, S. P. Gong, J. Y. Han and J. M. Lim. 2007. Establishment of a basic method for manipulating preantral follicles: effects of retrieval method on in vitro growth of preantral follicles and intrafollicular oocytes. Zygote 15:109-116   DOI   ScienceOn
7 Mao, J., G. Wu, M. F. Smith, T. C. McCauley, T. C. Cantley, R. S. Prather, B. A. Didion and B. N. Day. 2002. Effects of culture medium, serum type, and various concentrations of folliclestimulating hormone on porcine preantral follicular development and antrum formation in vitro. Biol. Reprod. 67:1197-1203   DOI   ScienceOn
8 Matzuk, M. M., K. H. Burns, M. M. Viveiros and J. J. Eppig. 2002. Intercellular communication in the mammalian ovary: oocytes carry the conversation. Sci. 296:2178-2180   DOI   PUBMED   ScienceOn
9 Rodr$\acute{i}$guez-Gonz$\acute{a}$lez, E., M. L$\acute{o}$pez-Bejar, M. J. Mertens and M. T. Paramio. 2003. Effects on in vitro embryo development and intracellular glutathione content of the presence of thiol compounds during maturation of prepubertal goat oocytes. Mol. Reprod. Dev. 65:446-453   DOI   ScienceOn
10 Lenie, S., R. Cortvrindt, T. Adriaenssens and J. Smitz. 2004. A reproductive two-step culture system for isolated primary mouse ovarian follicles as single functional units. Biol. Repod. 71:1730-1738   DOI   ScienceOn
11 Smitz, J. E. and R. G. Cortvrindt. 2002. The earliest stages of folliculogenesis in vitro. Reprod. 123:185-202   DOI   PUBMED   ScienceOn
12 Smitz, J., R. Cortvrindt, Y. Hu and H. Vanderstichele. 1998. Effects of recombinant activin A on in vitro culture of mouse preantral follicles. Mol. Reprod. Dev. 50:294-304   DOI   ScienceOn
13 Yang, J., M. Fu, S. Wang, X. Chen, G. Ning, B. Xu, Y. Ma, M. Zhang and G. Xia. 2008. An antisense oligodeoxynucleotide to the LH receptor attenuates FSH-induced oocyte maturation in mice. Asian-Aust. J. Anim. Sci. 21:972-979
14 Choi, M. H., J. H. Oh, T. M. Kim, J. Y. Han and J. M. Lim. 2006. Morphological criteria of bovine ovaries for predicting retrieval efficiency of preantral follicles. Asian-Aust. J. Anim. Sci. 19:1711-1715
15 Abeydeera, L. R., W. H. Wang, T. C. Cantley, R. S. Prather and B. N. Day. 1998. Presence of beta-mercaptoethanol can increase the glutathione content of pig oocytes matured in vitro and the rate of blastocyst development after in vitro fertilization. Theriogenol. 50:747-756   DOI   ScienceOn
16 Wu, J., B. R. Emery and D. T. Carrell. 2001. In vitro growth, maturation, fertilization, and embryonic development of oocytes from porcine preantral follicles. Biol. Reprod. 64:375-381   DOI   ScienceOn
17 Eppig, J. J. and A. C. Schroeder. 1989. Capacity of mouse oocytes from preantral follicles to undergo embryogenesis and development to live young after, growth, maturation, and fertilization in vitro. Biol. Reprod. 41:268-276   DOI   ScienceOn
18 Lim, J. M., S. S. Liou and W. Hansel. 1996. Intracytoplasmic glutathione concentration and the role of beta-mercaptoethanol in preimplantation development of bovine embryos. Theriogenol. 46:429-439   DOI   ScienceOn
19 Ohboshi, S., K. Hanada, J. Zhao, M. Hattori, N. Fujihara, R. Umetsu, T. Yoshida and H. Tomogane. 1996. In vitro development of bovine one-cell embryos fertilized in vitro in serum- and feeder cell-free culture systems. Asian-Aust. J. Anim. Sci. 9:583-590
20 Boland, N. I. and R. G. Gosden. 1994. Effects of epidermal growth factor on the growth and differentiation of cultured mouse ovarian follicles. J. Reprod. Fertil. 101:369-374   DOI   PUBMED
21 Ishii, T., I. Hishinuma, S. Bannai and Y. Sugita. 1981. Mechanism of growth promotion of mouse lymphoma L1210 cells in vitro by feeder layer or 2-mercaptoethanol. J. Cell Physiol. 107:283-293   DOI   PUBMED
22 Kim, I. W., S. P. Gong, C. R. Yoo, J. H. Choi, D. Y. Kim and J. M. Lim. 2008. Derivation of developmentally-competent oocytes by culturing of preantral follicles retrieved from adult ovaries: maturation, blastocyst formation, and embryonic stem cell transformation. Fertil. Steril. In press
23 Liu, X., K. Andoh, H. Yokota, J. Kobayashi, Y. Abe, K. Yamada, H. Mizunuma and Y. Ibuki. 1998. Effects of growth hormone, activin, and follistatin on the development of preantral follicle from immature female mice. Endocrinol. 139:2342-2347   DOI   PUBMED   ScienceOn
24 Telfer, E. E. 1996. The development of methods for isolation and culture of preantral follicles from bovine and porcine ovaries. Theriogenol. 45:101-110   DOI   ScienceOn
25 Hayashi, M., E. A. McGee, G. Min, C. Klein, U. M. Rose, M. van Duin and A. J. Hsueh. 1999. Recombinant growth differentiation factor-9 (GDF-9) enhances growth and differentiation of cultured early ovarian follicles. Endocrinol. 140:1236-1244   DOI   ScienceOn
26 Tao, Y., G. Xia, S. Bo, B. Zhou, M. Zhang and F. Wang. 2004. Nitric oxide exerts different functions on porcine oocytes cultured in different models, which is affected by betamercaptoethanol. Asian-Aust. J. Anim. Sci. 17:317-324   DOI   ScienceOn
27 Gu$\acute{e}$rin, P., S. El Mouatassim and Y. M$\acute{e}$n$\acute{e}$zo. 2001. Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings. Hum. Reprod. Update 7:175-189   DOI   ScienceOn
28 Donnelly, K. and E. E. Telfer. 1994. Growth of porcine preantral ovarian follicles in different culture systems. J. Reprod. Fertil. Abstract Series. 13:abstract 76   DOI   ScienceOn
29 Salmen, J. J., F. Skufca, A. Matt, G. Gushansky, A. Mason and C. S. Gardiner. 2005. Role of glutathione in reproductive tract secretions on mouse preimplantation embryo development. Biol. Reprod. 73:308-314   DOI   ScienceOn
30 Choi, J. K., J. H. Lee, S. T. Lee, M. H. Choi, S. P. Gong, E. J. Lee and J. M. Lim. 2007. Developmental competence of intrafollicular oocytes derived from preantral follicle culture with different protocols after parthenogenetic activation. Asian-Aust. J. Anim. Sci. 20:1190-1195
31 Eppig, J. J. and M. O'Brein. 1996. Development in vitro of mouse oocytes from primordial follicles. Biol. Reprod. 54:197-207   DOI   ScienceOn
32 Takahashi, M., T. Nagai, S. Hamano, M. Kuwayama, N. Okamura and A. Okano. 1993. Effect of thiol compounds on in vitro development and intracellular glutathione content of bovine embryos. Biol. Reprod. 49:228-232   DOI   ScienceOn
33 Cortvrindt, R., J. Smitz and A. C. Van Steirteghem. 1996. In-vitro maturation, fertilization and embryo development immature oocytes from early preantral follicles from prepuberal mice in a simplified culture system. Hum. Reprod. 11:2656-2666   DOI   ScienceOn
34 Gong, S. P., E. J. Lee, S. T. Lee, H. Kim, S. H. Lee, H. J. Han and J. M. Lim. 2008. Improved establishment of autologous stem cells derived from preantral follicle culture and oocyte parthenogenesis. Stem Cells Dev. 17:695-712   DOI   ScienceOn
35 Issels, R. D., A. Nagele, K. G. Eckert and W. Wilmanns. 1988. Promotion of cystine uptake and its utilization for glutathione biosynthesis induced by cysteamine and N-acetylcysteine. Biochem. Pharmacol. 37:881-888   DOI   ScienceOn
36 Choi, J. H., I. W. Kim, C. R. Yoo and J. M. Lim. 2008. Developmental competent oocytes growing in vitro culture of primary follicles: maturation and blastocyst formation after IVF and parthenogenetic activation. Fertil. Steril. Submitted
37 Choi, M. H., S. P. Gong and J. M. Lim. 2008. Retrieval of porcine ovarian follicles by different methods. Asian-Aust. J. Anim. Sci. 21:353-357   DOI   ScienceOn
38 Moon, S., S. P. Gong, E. J. Lee, W. K. Lee, J. M. Lim and H. Kim. 2008. Physical co-location between imprinting gene clusters and microRNAs and its impact on stem cell development. Genome Biol. Submitted
39 Wang, Z., S. Yu and Z. Xu. 2007. Effects of collection methods on recovery efficiency, maturation rate and subsequent embryonic developmental competence of oocytes in Holstein cow. Asian-Aust. J. Anim. Sci. 20:496-500