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http://dx.doi.org/10.5713/ajas.2009.90100

Dietary L-arginine Supplementation Improves Intestinal Function in Weaned Pigs after an Escherichia coli Lipopolysaccharide Challenge  

Liu, Yulan (State Key Laboratory of Animal Nutrition, China Agricultural University)
Han, Jie (Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University)
Huang, Jingjing (Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University)
Wang, Xiaoqiu (State Key Laboratory of Animal Nutrition, China Agricultural University)
Wang, Fenglai (State Key Laboratory of Animal Nutrition, China Agricultural University)
Wang, Junjun (State Key Laboratory of Animal Nutrition, China Agricultural University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.22, no.12, 2009 , pp. 1667-1675 More about this Journal
Abstract
This study was conducted to determine whether L-arginine (Arg) supplementation could improve intestinal function in weaned pigs after an Escherichia coli lipopolysaccharide (LPS) challenge. Treatments included: i) non-challenged control (CONTR, pigs fed a control diet and injected with sterile saline); ii) LPS-challenged control (LPS, pigs fed the same control diet and challenged by injection with Escherichia coli LPS); iii) LPS+0.5% Arg (pigs fed a 0.5% Arg diet and challenged with LPS); and iv) LPS+1.0% Arg (pigs fed a 1.0% Arg diet and challenged with LPS). On d 16, pigs were administrated with LPS or sterile saline. D-xylose was orally administrated at 2 h following LPS challenge, and blood samples were collected at 3 h following LPS challenge. At 6 h post-challenge, pigs were sacrificed and intestinal mucosa samples were collected. Supplementation of Arg attenuated LPS-induced damage in gut digestive and barrier functions, as indicated by an increase in ileal lactase activity, and duodenal and ileal diamine oxidase activities (p<0.05). Arg administration also prevented the increase of jejunal malondialdehyde content and the decrease of ileal superoxide dismutase activity by LPS challenge (p<0.05). Furthermore, the jejunal nitric oxide level and inducible nitric oxide synthase activity were also improved after Arg supplementation (p<0.05). These results indicate that Arg supplementation has beneficial effects in alleviating the impairment of gut function induced by LPS challenge.
Keywords
Arginine; Lipopolysaccharide; Weaned Pigs; Intestinal Function;
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