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http://dx.doi.org/10.5713/ajas.2009.80475

Differential Action of trans-10, cis-12 Conjugated Linoleic Acid on Adipocyte Differentiation of Ovine and 3T3-L1 Preadipocytes  

Iga, T. (Department of Food Production Science, Faculty of Agriculture, Shinshu University)
Satoh, T. (Department of Food Production Science, Faculty of Agriculture, Shinshu University)
Yamamoto, S. (Department of Food Production Science, Faculty of Agriculture, Shinshu University)
Fukui, K. (Department of Food Production Science, Faculty of Agriculture, Shinshu University)
Song, S.H. (Department of Animal Physiology, Graduate School of Agricultural Science, Tohoku University)
Choi, K.C. (National Institute of Animal Science)
Roh, S.G. (Department of Animal Physiology, Graduate School of Agricultural Science, Tohoku University)
Sasaki, S. (Department of Food Production Science, Faculty of Agriculture, Shinshu University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.22, no.11, 2009 , pp. 1566-1573 More about this Journal
Abstract
Trans-10, cis-12 conjugated linoleic acid (CLA) has been reported to inhibit the adipocyte differentiation of preadipocytes in non-ruminant animals (mice, rat, and human). However, the effects of trans-10, cis-12 CLA have not been clear in ruminants. The objective of this study was to investigate the effects of trans-10, cis-12 CLA on adipocyte differentiation of ovine preadipocytes. Differentiation of these preadipocytes was facilitated by treatment with trans-10, cis-12 CLA. Trans-10, cis-12 CLA increased the number and size of oil red O-stainable lipid drops as well as the levels of GPDH activity. PPAR-$\gamma{2}$ and adipophilin mRNA, adipogenic marker genes, were increased by treatment with trans-10, cis-12 CLA. This result was different from that observed with 3T3-L1 preadipocytes, a clonal cell line derived from rodents. Furthermore, trans-10, cis-12 CLA alone induced the adipocyte differentiation of ovine preadipocytes in differentiation-induction medium without troglitazone. These results suggest that CLA is an inducer and regulator in adipocyte differentiation of ovine preadipocytes, with species differences between ovine and rodent preadipocytes.
Keywords
trans-10; cis-12 CLA; Adipocyte; Sheep;
Citations & Related Records
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