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http://dx.doi.org/10.5713/ajas.2008.70532

Cloning, Expression, and Regulation of Bovine Cellular Retinoic Acid-binding Protein-II (CRABP-II) during Adipogenesis  

Jeong, Young Hee (Department of Food and Nutrition, Osaka City University)
Lee, Sang Mi (Department of Animal Science, Chonnam National University)
Kim, Hye-Min (Department of Animal Science, Chonnam National University)
Park, Hyo Young (Department of Animal Science, Chonnam National University)
Yoon, Duhak (National Livestock Research Institute, Rural Development Administration)
Moon, Seung Ju (Department of Animal Science, Chonnam National University)
Hosoda, Akemi (Department of Food and Nutrition, Osaka City University)
Kim, Dong-Ho (Department of Food and Nutrition, Osaka City University)
Saeki, Shigeru (Department of Food and Nutrition, Osaka City University)
Kang, Man-Jong (Department of Animal Science, Chonnam National University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.21, no.11, 2008 , pp. 1551-1558 More about this Journal
Abstract
The mammalian cellular retinoic acid-binding proteins, CRABP-I and CRABP-II, bind retinoic acid which acts as an inducer of differentiation in several biological systems. To investigate a possible role for CRABP-II in bovine adipogenesis, we have cloned bovine CRABP-II cDNA and the coding region for CRABP-I. The predicted amino acid sequences of CRABP-II were highly conserved among several animal species (human, mouse, and rat at 97%, 93%, and 93%, respectively). The expression pattern of bovine CRABP-II was examined in greater details by applying RT-PCR to various bovine tissues. CRABP-II mRNA was expressed in most adipose-containing tissues. Moreover, the expression of CRABP-I and -II mRNA dramatically increased during the differentiation of adipocytes from bovine intramuscular fibroblast-like cells. The effects of retinoic acid on adipocyte differentiation of bovine intramuscular fibroblast-like cells were concentration-dependent. Retinoic acid activated the formation of lipid droplets at a level of 1 nM, whereas inhibition was observed at a level of $1{\mu}M$. CRABP-I gene was up-regulated and CRABP-II gene down-regulated by retinoic acid during adipocyte differentiation. These results suggest that CRABPs may play an important role in the regulation of intracellular retinoic acid concentrations during adipogenesis.
Keywords
Bovine Adipocyte Differentiation; Lipid-binding Protein; Retinoic Acid; Retinoic Acid Receptor; Retinoid X Receptor;
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1 Suryawan, A. and C. Y. Hu. 1997. Effect of retinoic acid on differentiation of cultured pig preadipocytes. J. Anim. Sci. 75:112-117.   DOI
2 Tontonoz, P., R. A. Graves, A. I. Budavari, H. Erdjument-Bromage, M. Lui, E. Hu, P. Tempst and B. M. Spiegelman. 1994. Adipocyte-specific transcription factor ARF6 is a heterodimeric complex of two nuclear hormone receptors, $PPAR{\gamma}$ and $RXR{\alpha}$. Nucleic. Acids Res. 22:5628-5634.   DOI   ScienceOn
3 Tontonoz, P., S. Singer, B. M. Forman, P. Sarraf, J. A. Fletcher, C. D. Fletcher, R. P. Brun, E. Mueller, S. Altiok, H. Oppenheim, R. M. Evans and B. M. Spiegelman. 1997. Terminal differentiation of human liposarcoma cells induced by ligands for peroxisome proliferator-activated receptor gamma and the retinoid X receptor. Proc. Natl. Acad. Sci. USA. 94:237-241.   DOI
4 Wardlaw, S. A., R. A. Bucco, W. L. Zheng and D. E. Ong. 1997. Variable expression of cellular retinol-and cellular retinoic acid-binding proteins in the rat uterus and ovary during the estrous cycle. Biol. Reprod. 56:125-132.   DOI   ScienceOn
5 Xue, J. C., E. J. Schwarz, A. Chawla and M. A. Lazar. 1996. Distinct stages in adipogenesis revealed by retinoid inhibition of differentiation after induction of $PPAR{\gamma}$. Mol. Cell Biol. 16:1567-1575.   DOI
6 Zheng, W. L. and D. E. Ong. 1998. Spatial and temporal patterns of expression of cellular retinol-binding protein and cellular retinoic acid-binding proteins in rat uterus during early pregnancy. Biol. Reprod. 58:963-970.   DOI   ScienceOn
7 Gorocica-Buenfil, M. A., F. L. Fluharty, C. K. Reynolds and S. C. Loerch. 2007b. Effect of dietary vitamin A restriction on marbling and conjugated linoleic acid content in Holstein steers. J. Anim. Sci. 85:2243-2255.   DOI   ScienceOn
8 Aso, H., H. Abe, I. Nakajima, K. Ozutsumi, T. Yamaguchi, Y. Takamori, A. Kodama, F. B. Hoshino and S. Takano. 1995. A preadipocyte clonal line from bovine intramuscular adipose tissue: nonexpression of Gult-4 protein during adipocyte differentiation. Biochem. Biophys. Res. Commun. 213:369-375.   DOI   ScienceOn
9 Astrom, A., A. Tavakkol, U. Pettersson, M. Cromie, J. T. Elder and J. J. Voorhees. 1991. Molecular cloning of two human cellular retinoic acid-binding proteins (CRABP). Retinoic acid-induced expression of CRABP-II but not CRABP-I in adult human skin in vivo and in skin fibroblasts in vitro. J. Biol. Chem. 266:17662-17666.
10 Astrom, A., U. Pettersson and J. J. Voorhees. 1992. Structure of the human cellular retinoic acid-binding protein II gene. Early transcriptional regulation by retinoic acid. J. Biol. Chem. 267:25251-25255.
11 Gorocica-Buenfil, M. A., F. L. Fluharty, T. Bohn, S. J. Schwartz and S. C. Loerch. 2007c. Effect of low vitamin A diets with high-moisture or dry corn on marbling and adipose tissue fatty acid composition of beef steers. J. Anim. Sci. 85:3355-3366.   DOI   ScienceOn
12 Okuno, M., E. Arimoto, M. Nishizuka, T. Nishihara and M. Imagawa. 2002. Isolation of up- or down-regulated genes in $PPAR{\gamma}$-expressing NIH-3T3 cells during differentiation into adipocytes. FEBS Lett. 519:108-112.   DOI   ScienceOn
13 Ong, D. E., M. E. Newcomer and F. Chytil. 1993. The retinoids, Vol. 2. Academic Press, New York. pp. 283-318.
14 Chambon, P. 1996. A decade of molecular biology of retinoic acid receptors. FASEB J. 10:940-954.   DOI
15 Safonova, I., C. Darimont, E. Z. Amri, P. Grimaldi, G. Ailhaud, U. Reichert and B. Shroot. 1994. Retinoids are positive effectors of adipose cell differentiation. Mol. Cell Endocrinol. 104:201-211.   DOI   ScienceOn
16 Bruck, N., J. Bastien, G. Bour, A. Tarrade, J. L. Plassat, A. Bauer, S. Adam-Stitah and C. Rochette-Egly. 2005. Phosphorylation of the retinoid x receptor at the omega loop, modulates the expression of retinoic-acid-target genes with a promoter context specificity. Cell Signal. 17:1229-1239.   DOI   ScienceOn
17 Bucco, R. A., M. H. Melner, D. S. Gordon, S. Leers-Sucheta and D. E. Ong. 1995. Inducible expression of cellular retinoic acid-binding protein II in rat ovary: gonadotropin regulation during luteal development. Endocrinol. 136:2730-2740.   DOI   ScienceOn
18 Cowherd, R. M., R. E. Lyle and R. E. Jr. McGehee. 1999. Molecular regulation of adipocyte differentiation. Semin. Cell Dev. Biol. 10:3-10.
19 Dani, C., A. G. Smith, S. Dessolin, P. Leroy, L. Staccini, P. Villageois, C. Darimont and G. Ailhaud. 1997. Differentiation of embryonic stem cells into adipocytes in vitro. J. Cell Sci. 110:1279-1285.
20 Dong, D., S. E. Ruuska, D. J. Levinthal and N. Noy. 1999. Distinct roles for cellular retinoic acid-binding proteins I and II in regulating signaling by retinoic acid. J. Biol. Chem. 274:23695-23698.   DOI
21 Giguere, V., S. Lyn, P. Yip, C. H. Siu and S. Amin. 1990. Molecular cloning of cDNA encoding a second cellular retinoic acid-binding protein. Proc. Natl. Acad. Sci. USA. 87:6233-6237.   DOI   ScienceOn
22 Li, E. and A. W. Norris. 1996. Structure/function of cytoplasmic vitamin A-binding proteins. Annu. Rev. Nutr. 16:205-234.   DOI   ScienceOn
23 Gorocica-Buenfil, M. A., F. L. Fluharty, C. K. Reynolds and S. C. Loerch. 2007a. Effect of dietary vitamin A concentration and roistered soybean inclusion on marbling, adipose cellularity, and fatty acid composition of beef. J. Anim. Sci. 85:2230-2242.   DOI   ScienceOn
24 Kawada, T., N. Aoki, Y. Kamei, K. Maeshige, S. Nishiu and E. Sugimoto. 1990. Comparative investigation of vitamins and their analogues on terminal differentiation, from preadipocytes to adipocytes, of 3T3-L1 cells. Comp. Biochem. Physiol. 96:323-326.   DOI   ScienceOn
25 Lee, H. J., S. C. Lee, Y. K. Oh and In K. Han. 2000. Effects of vitamins on the differentiation of preadipocytes from Hanwoo cattle adipose tissues. Asian-Aust. J. Anim. Sci. 13:446-450.   DOI
26 Means, A. L., J. R. Thompson and L. J. Gudas. 2000. Transcriptional regulation of the cellular retinoic acid binding protein I gene in F9 teratocarcinoma cells. Cell Growth Differ. 11:71-82.
27 Miano, J. M. and B. C. Berk. 2000. Retinoids: versatile biological response modifiers of vascular smooth muscle phenotype. Circ. Res. 87:355-362.   DOI   ScienceOn
28 Napoli, J. L. 1996. Retinoic acid biosynthesis and metabolism. FASEB J. 10:993-1001.   DOI
29 Ohyama, M., K. Matsuda, S, Torii, T. Matsui, H. Yano, T. Kawada and T. Ishihara. 1998. The interation between vitamin A and thiazolidinedione on bovine adipocyte differentiation in primary culture. J. Anim. Sci. 76:61-65.   DOI
30 Oka, A., Y. Maruo, T. Miki, T. Yamasaki and T. Saito. 1998. Influence of vitamin A on the quality of beef from the Tajima strain of Japanase black cattle. Meat Sci. 48:150-167
31 Kamei, Y., T. Kawada, J. Mizukami and E. Sugimoto. 1994. The prevention of adipose differentiation of 3T3-L1 cells caused by retinoic acid is elicited through retinoic acid receptor alpha. Life Sci. 55:307-312.
32 Bastien, J., S. Adam-Stitah, J. L. Plassat, P. Chambon and C. Rochette-Egly. 2002. The phosphorylation site located in the A region of retinoic X receptor alpha is required for the antiproliferative effect of retinoic acid (RA) and the activation of RA target genes in F9 cells. J. Biol. Chem. 277:28683-28689.   DOI   ScienceOn
33 Haq, R. and F. Chytil. 1991. Expression of nuclear retinoic acid receptors in rat adipose tissue. Biochem. Biophys. Res. Commun. 176:1539-1544.   DOI   ScienceOn
34 Kamei, Y., T. Kawada, R. Kazuki and E. Sugimoto. 1993. Retinoic acid receptor gamma 2 gene expression is up-regulated by retinoic acid in 3T3-L1 preadipocytes. Biochem. J. 293:807-812.   DOI