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http://dx.doi.org/10.5713/ajas.2007.1417

Supplementation Effects of $C_{18:2}$ or $C_{18:3}$ Rich-oils on Formations of CLA and TVA, and Lipogenesis in Adipose Tissues of Sheep  

Choi, S.H. (Department of Animal Science, Chungbuk National University)
Lim, K.W. (Department of Animal Science, Chungbuk National University)
Lee, H.G. (School of Agricultural Biotechnology, Seoul National University)
Kim, Y.J. (Department of Food and Biotechnology, Korea University)
Song, Man K. (Department of Animal Science, Chungbuk National University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.20, no.9, 2007 , pp. 1417-1423 More about this Journal
Abstract
The present study was conducted to investigate the supplementation effects of $C_{18:2}$ rich-soybean oil or $C_{18:3}$ rich-perilla oil (7% of total diet, DM basis) for 12 weeks on plasma metabolites, fatty acid profile, in vitro lipogenesis, and activities of LPL and FAS in adipose tissue of sheep. The treatments were basal diet (Control), $C_{18:2}$ rich-soybean oil supplemented diet (SO-D) and $C_{18:3}$ rich-perilla oil supplemented diet (PO-D). All the sheep were fed the diets consisting of roughage to concentrate in the ratio of 40:60 (DM basis). Oil supplemented diets (SO-D and PO-D) slightly increased contents of triglyceride (TG) and total cholesterol (TC), proportions of both cis-9 trans-11 and trans-10 cis-12 CLA and TVA, but lowered (p<0.01) those of $C_{18:0}$ compared to the control diet. No differences were observed in the contents of TG and TC and proportions of fatty acids in plasma between supplemented oils. Oil supplemented diets slightly increased the proportions of cis-9 trans-11 and trans-10 cis-12 types of CLA in subcutaneous adipose tissue of sheep compared to the control diet. The rate of lipogenesis with acetate was higher (p<0.01) for intermuscular- and subcutaneous adipose tissues than that for intramuscular adipose tissue, while that with glucose did not differ among fat locations in sheep fed SO-D. No differences were observed in the rate of lipogenesis between substrates in all fat locations. The rates of lipogenesis with glucose increased only in the intermuscular- (p<0.01) and subcutaneous adipose tissue (p<0.005) compared to those with acetate. The rates of lipogenesis with acetate were the highest in the intermuscular and intramuscular adipose tissue of the sheep fed PO-D. Oil supplemented diets slightly increased the rate of lipogenesis with glucose for all fat locations. Supplementation of oils to the diet numerically increased the fatty acid synthase activity but did not affect the lipoprotein lipase activity in subcutaneous adipose tissue.
Keywords
Conjugated Linoleic Acid; Oils; Lipogenesis; Lipogenic Enzymes; Adipose Tissue; Sheep;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
Times Cited By Web Of Science : 6  (Related Records In Web of Science)
Times Cited By SCOPUS : 6
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1 Garcia, M. R., M. Amstalden, C. D. Morrison, D. H. Keisler and G. L. Williams. 2003. Age at puberty, total fat and conjugated linoleic acid content of carcass, and circulating metabolic hormones in beef heifers fed a diet high in linoleic acid beginning at four months of age. J. Anim. Sci. 81(1):261-268   DOI
2 Griinari, J. M., B. A. Corl, S. H. Lacy, P. Y. Chouinard, K. V. Nurmela and D. E. Bauman. 2000. Conjugated linoleic acid is synthesized endogenously in lactating dairy cows by delta (9)-desaturase. J. Nutr. 130:2285-2291   DOI
3 Iverius, P. H. and J. D. Brunzell. 1985. Human adipose tissue lipoprotein lipase: changes with feeding and relation to postheparin plasma enzyme. Am. J. Physiol. Endocrinol Metabolism. 249:E107-E114   DOI
4 Park, Y., J. J. Albright, J. M. Storkson, M. E. Cook and M. W. Pariza. 1997. Effects of conjugated linoleic acid on body composition in mice. Lipids 32:853-858   DOI   ScienceOn
5 Wang, J. H., S. H. Choi, K. W. Lim, K. H. Kim and M. K. Song. 2006. Effect of the mixed oil and monensin supplementation, and feeding duration of supplements on c9, t11-CLA contents in plasma and fat tissues of Korean native (Hanwoo) steers. Asian-Aust. J. Anim. Sci. 19:1464-1469   과학기술학회마을   DOI
6 Choi, S. H., G. W. Lim, G. L. Jin and M. K. Song. 2006b. Effect of the level of carbohydrates on bio-hydrogenatin and CLA production by rumen bacteria when incubated with soybean oil or flaxseed oil in vitro. Kor. J. Anim. Sci. Technol. 48:521-532   DOI   ScienceOn
7 Kott, R. W., P. G. Hatfield, J. W. Bergman, C. R. Flynn, H. V. Wagoner and J. A. Boles. 2003. Feedlot performance, carcass composition, and muscle and fat CLA concentration of lambs fed diets supplemented with safflower seed. Small Rumin. Res. 49:11-17   DOI   ScienceOn
8 Song, M. K., H. J. Sohn, S. K. Hong and H. C. Kim. 2001. Utilization of substrate of the in vitro lipid synthesis in the adipose tissue of Hanwoo steers. Asian-Aust. J. Anim. Sci. 14: 1560-1563   DOI
9 Steel, R. G. D. and J. H. Torrie. 1980. Principles and procedures of statistics. Mcgraw Hill Book Co., NY
10 Tsuboyama-Kasaoka, M., M. Takahashi, K. Tanemura, H. J. Kim, T. Tange, H. Okuyama, M. Kasai, S. Ikemoto and O. Ezaki. 2000. Conjugated linoleic acid supplementation reduces adipose tissue by apoptosis and develops lipodystrophy in mice. Diabetes. 49:1534-1542   DOI   ScienceOn
11 Lepage, G. and C. Roy. 1986. Direct trans-esterification of all classes of lipids in a one step reaction. J. Lipid Res. 27:114-120   DOI   ScienceOn
12 Mir, Z., L. A. Goonewardene, E. Okine, S. Jaegar and H. D. Scheer. 1999. Effect of feeding canola oil on constituents, conjugated linoleic acid (CLA) and long chain fatty acids in goats milk. Small Rumin. Res. 33:137-143   DOI   ScienceOn
13 Nilsson-Ehle, P. and M. C. Schotz. 1976. A Stable Radioactive Emulsion for Assay of Lipoprotein Lipase. J. Lipid Res. 17:536-541
14 NRC. 1985. Nutritional requirement of sheep. 6th ed. Natl. Acad. Press, Washington, DC
15 Ostrowska, E., M. Muralitharan, R. F. Cross, D. E. Bauman and F. R. Dunshea. 1999. Dietary conjugated linoleic acids increase lean tissue and decrease fat deposition in growing pigs. J. Nutr. 129:2037-2042   DOI
16 Faulconnier, Y., M. Thevenet, J. Flechet and Y. Chilliard. 1994. Lipoprotein lipase and metabolic activities in incubated bovine adipose tissue explants: Effects of insulin, dexamethasone, and fetal bovine serum. J. Anim. Sci. 72:184-191   DOI
17 Folch, J., M. Lee and G. H. Sloan-Stanley. 1957. A simple method for the isolation and purification of total lipids from animal tissue. J. Biol. Chem. 226:497-509
18 Vernon, R. G. 1979. Lipogenesis in Ovine Adipose Tissue in Tissue Culture. Ann. Rech. Vet. 10:399-400
19 Wang, J. H., S. H. Choi, C. G. Yan and M. K. Song. 2005. Effect of monensin and fish oil supplementation on biohydrogenation and CLA production by rumen bacteria in vitro when incubated with safflower oil. Asian-Aust. J. Anim. Sci. 18:221-225   과학기술학회마을   DOI
20 Vernon, R. G. 1976. Effct of dietary fats on ovine adipose tissue metabolism. Lipids. 11:662-669   DOI   ScienceOn
21 Wang, J. H., M. K. Song, Y. S. Son and M. B. Chang. 2002a. Effect of concentrate level on the formation of conjugated linoleic acid and trans-octadecenoic acid by ruminal bacteria when incubated with oilseeds in vitro. Asian-Aust. J. Anim. Sci. 15:687-694
22 Wang, J. H. and M. K. Song. 2002b. Effect of concentrate to roughage ratio and oil source on the formation of $t-11C_{18:1}$ and c-9, $t-11C_{18:2}$ in rumen fluid and plasma of sheep. The 4th Korea-Japan Joint Symposium on Rumen Metabolism and Physiology in Jeju, Korea. p. 113 (Abst.)
23 Wang, J. H., S. H. Choi and M. K. Song. 2003. pH affects the in vitro formation of cis-9, trans-11 CLA and trans-11 Octadecenoic acid by ruminal bacteria when incubated with oilseeds. Asian-Aust. J. Anim. Sci. 16:1743-1748   과학기술학회마을   DOI
24 Park, Y., J. M. Storkson, K. J. Albright, K. S. Liu and M. W. Pariza. 1999. Evidence that the trans-10, cis-12 Isomer of Conjugated Linoleic Acid Induces Body Composition Changes in Mice. Lipids. 34:235-241   DOI   ScienceOn
25 SAS. 1985. SAS User's Guide. Statistical analysis systems institute, Inc. Cary, NC
26 Dole, V. P. 1956. A relation between non-esterified fatty acids in plasma and the metabolism of glucose. J. Clin. Invest. 35(2):150-154   DOI   ScienceOn
27 Smith, S. B. and J. D. Crouse. 1984. Relative Contribution of Acetate, Lactate and Glucose to Lipogenesis in Bovine Intramuscular and Subcutaneous Adipose Tissue. J. Nutr. 114:792   DOI
28 Corino, C., J. Mourot, S. Magni, G. Pastorelli and F. Rosi. 2002. Influence of dietary conjugated linoleic acid on growth, meat quality, lipogenesis, plasma leptin and physiological variables of lipid metabolism in rabbits. J. Anim. Sci. 80:1020-1028   DOI
29 Cryer, A. 1981. Tissue lipoprotein lipase activity and its action in lipoprotein metabolism. Int. J. Biochem. 13:525-541   DOI   ScienceOn
30 Bee, G. 2001. Dietary conjugated linoleic acids effect tissue lipid composition but not de novo lipogenesis in finishing pigs. Anim. Res. 50:383-399   DOI   ScienceOn
31 Belfrage, P. and M. Vaughan. 1969. Simple liquid-liquid partition system for isolation of labeled oleic acid from mixtures with glycerides. J. Lipid. Res. 10:341-344
32 Brown, M., M. Evans and M. McIntsh. 2001. Linoleic acid partially restores the triglyceride content of conjugated linoleic acid-treated cultures of 3T3-L1 preadipocytes, J. Nutr. Biochem. 12:381-387   DOI   ScienceOn
33 Chant, J. H. P., D. K. Lunt and S. B. Smith. 1992. Fatty acid composition and fatty acid elongase and stearoyl-CoA desaturase activities in tissues of steers fed high oleate sunflower seed. J. Nutr. 122:2074-2080   DOI
34 Choi, S. H., J. H. Wang, Y. J. Kim, Y. K. Ohh and M. K. Song. 2006a. Effect of soybean oil supplementation on the contents of plasma cholesterol and cis9, trans11-CLA of the fat tissues in sheep. Asian-Aust. J. Anim. Sci. 19:679-683   DOI
35 Cook, M., D. Jerome, T. Crenshaw, P. Buege, M. Pariza, K. Albright, S. Schmidt, J. Scimeca, P. Lotgren and E. Hentges. 1999. Feeding conjugated linoleic acid improves feeding efficiency and reduces carcass fat in pig, adipocyte biology and hormone signaling symposium. Univ. Wisconsin-Madison, Dept. Biochem. p. 67
36 Bassaganya-Riera, J. and R. Hontecillas. 2006. CLA and n-3 PUFA differentially modulate clinical activity and colonic PPAR-responsive gene expression in a pig model of experimental IBD. Clinic. Nutr. 25:454-465   DOI   ScienceOn
37 Choi, S. H. and M. K. Song. 2005. Effect of $C_{18}-polyunsaturated$ fatty acids on their direct incorporation into the rumen bacterial lipids and CLA production in vitro. Asian-Aust. J. Anim. Sci. 18:512-515   DOI
38 Chung, C. S., N. S. Kim, M. K. Song, Y. I. Choi, Y. S. Woon, J. K. Chung and J. G. Kim. 2000. Effects of age and feeding level of concentrates on adipose tissue lipogenesis and adipocyte size in Hanwoo bulls. Kor. J. Anim. Sci. Technol. 42(4):459-466