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http://dx.doi.org/10.5713/ajas.2007.856

Development and Characterization of a Specific Anti-Caveolin-1 Antibody for Caveolin-1 Functional Study in Human, Goat and Mouse  

Ke, Meng-Wei (Department of Animal Science and Technology, National Taiwan University)
Jiang, Yan-Nian (Department of Animal Science and Technology, National Taiwan University)
Li, Yi-Hung (Department of Animal Science and Technology, National Taiwan University)
Tseng, Ting-Yu (Department of Animal Science and Technology, National Taiwan University)
Kung, Ming-Shung (Department of Animal Science and Technology, National Taiwan University)
Huang, Chiun-Sheng (Department of Surgery, National Taiwan University Hospital and National Taiwan University of College of Medicine)
Cheng, Winston Teng-Kuei (Department of Animal Science and Technology, National Taiwan University)
Hsu, Jih-Tay (Department of Animal Science and Technology, National Taiwan University)
Ju, Yu-Ten (Department of Animal Science and Technology, National Taiwan University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.20, no.6, 2007 , pp. 856-865 More about this Journal
Abstract
Caveolin-1 of the caveolin family of proteins regulates mammary gland development and has been shown to play a contradictory role in breast tumor progression. A specific anti-Caveolin-1 antibody will be useful for functional study of Caveolin-1 in different tissues. In this study, we generated a rabbit polyclonal antibody that specifically recognizes the N-terminal amino acids 50-65 of Caveolin-1. This polyclonal antibody specifically reacted with Caveolin-1 extracted from cells of different species, including human epithelial A431 cells, goat primary mammary epithelial cells and mice fibroblast NIH 3T3 cells, by Western blotting. Endogenous Caveolin-1 protein expressing in cells and normal human tissues was detected by this polyclonal antibody using immunocytofluorescent and immunohistochemical staining, respectively. Furthermore, an apparent decrease in Caveolin-1 expression in tumorous breast and colon tissues was detected by this polyclonal antibody. In conclusion, we have identified amino acids 50-65 of Caveolin-1, which contains an epitope that is specific to Caveolin-1 and is conserved in the human, goat and mouse. In future, this anti-Caveolin-1 antibody can be used to examine the progression of breast and colon cancers and to study functions of Caveolin-1 in human, goat and mouse cells.
Keywords
Caveolin-1; Peptide Antibody; Breast Cancer; Goat; Cancer Marker;
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