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http://dx.doi.org/10.5713/ajas.2007.817

Association of Sequence Variations in DGAT 1 Gene with Economic Traits in Hanwoo (Korea Cattle)  

Kong, H.S. (Genomic Informatics Center, Hankyong National University)
Oh, J.D. (Genomic Informatics Center, Hankyong National University)
Lee, J.H. (Genomic Informatics Center, Hankyong National University)
Yoon, D.H. (National Livestock Research Institute, RDA)
Choi, Y.H. (National Livestock Research Institute, RDA)
Cho, B.W. (Pusan National University)
Lee, H.K. (Genomic Informatics Center, Hankyong National University)
Jeon, G.J. (Genomic Informatics Center, Hankyong National University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.20, no.6, 2007 , pp. 817-820 More about this Journal
Abstract
The effects of diacylglycerol O-acyltransferase (DGAT1) candidate gene polymorphism on the economic traits of Hanwoo were studied. Through sequencing analysis, two polymorphism sites at K232A and T11993C were established and were analyzed by PCR-RFLP. The PCR-RFLP analysis for K232A showed that the frequencies of alleles K and A were 0.75 and 0.25, respectively, and the frequencies of genotypes for K/K, K/A and A/A were estimated as 0.509, 0.491 and 0, respectively. In the PCR-RFLP analysis for T11993C, we found allele frequencies of 0.773 and 0.227 for T and A, respectively, and 0.546, 0.454 and 0 for the T/T, T/C and C/C genotype frequencies, respectively. No significant effects on economic traits in Hanwoo were found in the separate analysis of K232A and T11993C polymorphisms, but the interaction between K232A and T11993C showed a significant effect (p<0.005) on marbling score. The DGAT1 candidate gene was found to have a significant effect not only on milk yield and component traits but also on the metabolism of intramuscular fat.
Keywords
DGAT1 Gene; Single Nucleotide Polymorphism (SNP); Hanwoo;
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1 Cases, S., S. J. Smith, Y. W. Zheng, H. M. Myers, S. R. Lear, E. Sande, S. Novak, C. Collins, C. B. Welch, A. J. Lusis, S. K. Erickson and R. V. Farese Jr. 1998. Identification of a gene encoding an acyl CoA:diacylglycerol acyltransferase, a key enzyme in triacylglycerol synthesis, Proc. Natl. Acad. Sci. USA. 95:13018-13023.   DOI
2 Coppieters, W., J. Riquet, J. J. Arranz, P. Berzi, N. Cambisano, B. Grisart, L. Karim, F. Marcq, L. Moreau, C. Nezer, P. Simon, P. Vanmanshoven, D. Wagnaar and M. Georges. 1998. A QTL with major effect on milk yield and composition maps to bovine chromosome 14. Mamm. Genome. 9(7):540-544.   DOI   ScienceOn
3 SAS. 2002-2003. SAS/SAT User's Guide, Version 9.1. SAS Institute Inc., Cary, NC, USA.
4 Spelman, R. J., C. A. Ford, P. McElhinney, G. C. Gregory and R. G. Snell. 2002. Characterization of the DGAT1 gene in the New Zealand dairy population. J. Dairy Sci. 85(12):3514-3517.   DOI   ScienceOn
5 Farnir, F., B. Grisat, W. Coppieters, J. Riquet, P. Berzi, N. Cambisano, L. Karim, M. Mni, P. Simon and D. Wagenaar. 2002. Simultaneous mining of linkage and linkage disequilibrium to fine map quantitative trait loci in outbred half-sib pedigrees: revisiting the location of a quantitative trait locus with major effect on milk production on bovine chromosome 14. Genet. 161(1):275-287.
6 Thaller, G., C. Kuhn, A. Winter, G. Ewald, O. Bellmann, J. Wegner, H. Zuhlke and R. Fries. 2003. DGAT1, a new positional and functional candidate gene for intramuscular fat deposition in cattle. Anim Genet. Oct;34(5):354-7.   DOI   ScienceOn
7 Chung, E. R. and W. T. Kim. 2005. Association of SNP Marker in IGF-I and MYF5 Candidate Genes with Growth Traits in Korean Cattle. Asian-Aust. J. Anim. Sci. 18(8):1061-1065.   과학기술학회마을   DOI
8 Riquet, J., W. Coppieters, N. Cambisano, J. J. Arranz, P. Berzi, S. K. Davis, B. Grisart, F. Farnir, L. Karim, M. Mni, P. Simon, J. F. Tayor, P. Vanmanshoven, D. Wagenaar, J. E. Womack and M. Georges. 1999. Fine-mapping of quantitative trait loci by identity by desent in outbred populations: application to milk production in dairy cattle. Proc. Natl. Acad. Sci. USA. AUG 3;96(16):9252-9257.   DOI
9 Smith, S. J., S. Cases, D. R. Jensen, H. C. Chen, E. Sande, B. Tow, D. A. Sanan, J. Raber, R. H. Eckel and R. V. Jr. Farese. 2000. Obesty resistance and multiple mechanisms of triglyceride synthesis in mice laking DGAT1. Nat. Genet. 25:87-90.   DOI   ScienceOn
10 Sambrook, J., E. F. Fritsch and T. Maniatis. 1989. Molecular cloning a laboratory manual. 2nd Ed. Cold Spring Harbor Lababoratory
11 Winter, A., W. Kramer, F. A. O. Werner, S. Kollers, S. Kata, G. Durstewitz, J. Buitkamp, J. E. Womack, G. Thaller and R. Fries. 2002. Assotion of a lysine-232/alanine polymorphism in a bovine gene encoding acyl coA:diacylglycerol acyltransferase (DGAT1) with variation at a quantitative trait locus for milk fat content. Proceedings of the National Academy of Sciences of the United States of America 99:9300-9505.   DOI   ScienceOn
12 Kong, H. S., J. D. Oh, S. G. Lee, Y. S. Hong, W. I. Song, S. J. Lee, H. C. Kim, B. H. Yoo, H. K. Lee and G. J. Jeon. 2006. Association of Polymorphisms in the Bovine Leptin Gene with Ultrasound Measurements for Improving in Korean Cattle. Asian-Aust. J. Anim. Sci. 19(12):1691-1695.   과학기술학회마을   DOI
13 Miller, S. A., D. D. Dykes and H. F. Polesky. 1988. A simple salting out procedure for extracting DNA from human nucleated cell. Nucleic Acids Res. 16:1215.   DOI   ScienceOn
14 Heyen, D. W., J. I. Weller, M. Ron, M. Band, J. E. Beever, E. Feldmesser, Y. Da, G. R. Wiggans, P. M. Vanradenm and H. A. Lewin. 1999. A genome scan for QTL influencing milk production and health trait in dairy cattle. Physiol. Genomics 1:165-175.   DOI
15 Looft, C., N. Reinsch, C. Karall-Albrecht, S. Paul, M. Brink, H. Thomsen, G. Brockman, C. Khun, M. Schwerin and E. Kalm. 2001. A mammary gland EST showing linkage disequilibrium to a milk production QTL on bovine chromosome 14. Mamm. Genome. 12(8):646-50.   DOI   ScienceOn
16 Shin, S. C. and E. R. Chung. 2007. Association of SNP Marker in the Leptin Gene with Carcass and Meat Quality Traits in Korean Cattle. Asian-Aust. J. Anim. Sci. 20(1):1-6.   과학기술학회마을
17 Schrooten, C. and H. Bovenhuis. 2002 Detection of pleiotropic effects of quantitative trait loci in outbred populations using regression analysis. J Dairy Sci. 85(12):3503-3513.   DOI   ScienceOn
18 Grisart, B., W. Coppieters, F. Farnir, L. Karim, C. Ford, P. Berzi, N. Cambisano, M. Mni, S. Reid, P. Simon, R. Spelman, M. Georges and R. Snell. 2002. Positional candidate cloning of a QTL in dairy cattle: identification of a missense mutation in the bovine DGAT1 gene with major effect on milk yield and composition. Genome. Res. 12(2):222-231.   DOI   ScienceOn
19 Cheong, H. S., D. H. Yoon, A. H. Kim, B. L. Park, E. R. Chung, H. J. Lee, I. C. Cheong, S. J. Oh and H. D. Shin. 2006. Leptin Polymorphisms Associated with Carcass Traits of Meat in Korean Cattle. Asian-Aust. J. Anim. Sci. 19(11):1529-1535.   과학기술학회마을   DOI
20 Sonstegard, T. S., C. P. Van Tassell and M. S. Ashwell. 2001. Dairy cattle genomics: Tools to accelerate genetics improvement. J. Anim. Sci. 79(Suppl. E):307-315.   DOI