Browse > Article
http://dx.doi.org/10.5713/ajas.2006.953

Molecular Characterization and Expression Patterns of Porcine Eukaryotic Elongation Factor 1 A  

Wang, H.L. (Department of Gene and Cell Engineering, Institute of Animal Science Chinese Academy of Agricultural Sciences)
Wang, H. (Department of Gene and Cell Engineering, Institute of Animal Science Chinese Academy of Agricultural Sciences)
Zhu, Z.M. (Department of Gene and Cell Engineering, Institute of Animal Science Chinese Academy of Agricultural Sciences)
Yang, S.L. (Department of Gene and Cell Engineering, Institute of Animal Science Chinese Academy of Agricultural Sciences)
Fen, S.T. (Department of Gene and Cell Engineering, Institute of Animal Science Chinese Academy of Agricultural Sciences)
Li, Kui (Department of Gene and Cell Engineering, Institute of Animal Science Chinese Academy of Agricultural Sciences)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.19, no.7, 2006 , pp. 953-957 More about this Journal
Abstract
The eukaryotic elongation factor 1 A (EEF1A) participates in protein synthesis by forming the eEF1A GTP tRNA complex to deliver aminoacyl-tRNA to the A site of ribosomes. This study described cDNA sequences and partial genomic structure of porcine EEF1A1. The porcine EEF1A1 gene encoded a protein with 462 amino acids, which shared complete homology with human, chimpanzee and dog. The temporal expression pattern showed the diversity of EEF1A1 level in mRNA was relatively minor in prenatal embryo skeletal muscle, however, the expression decreased during aging after birth in skeletal muscle of the Chinese Tongcheng pig. The spatial expression patterns indicated that the gene expressed in skeletal muscle, heart, lung, liver, kidney, fat and spleen. In addition, we assigned the gene to porcine chromosome 1 using a radiation hybrid panel.
Keywords
EEF1A1; Expression; Chromosome; Genome; Porcine;
Citations & Related Records

Times Cited By Web Of Science : 2  (Related Records In Web of Science)
Times Cited By SCOPUS : 2
연도 인용수 순위
1 Shao, M. Y., H. Wang, H. Y. Ren, Z. M. Zhu, S. L. Yang and K. Li. 2006. Assignment of CRSP9, ETF1 and TMEM59 genes to porcine chromosomes. Cytogenet Genome Res. 112:3421 (DOI.10.1159/000089899)
2 Cavallius, J., S. I. Rattan and B. F. Clark. 1986. Changes in activity and amount of active elongation factor 1 alpha in aging and immortal human fibroblast cultures. Exp. Gerontol. 21:149-157   DOI   ScienceOn
3 Chiron, S., A. Suleau and N. Bonnefoy. 2005. Mitochondrial Translation Elongation Factor Tu Is Essential in Fission Yeast and Depends on an Exchange Factor Conserved in Humans but Not in Budding Yeast. Genet. 169:1891-1901   DOI   ScienceOn
4 Milan, D., R. Hawken, C. Cabau, S. Leroux, C. Genet, Y. Lahbib, G. Tosser, A. Robic, F. Hatey, L. Alexander, C. Beattie, L. Schook, M. Yerle and J. Gellin. 2000. IMpRH Server: an RH mapping server available on the Web. Bioinformatics. 16:558-559   DOI
5 Knudsen, C., H. J. Wieden and M. V. Rodnina. 2001. The Importance of Structural Transitions of the Switch II Region for the Functions of Elongation Factor Tu on the Ribosome. J. Biol. Chem. 276:22183-22190   DOI   ScienceOn
6 Carr-Schmid, A., N. Durko, J. Cavallius, W. C. Merrick and T. G. Kinzy. 1999. Mutations in a GTP-binding Motif of Eukaryotic Elongation Factor 1A Reduce Both Translational Fidelity and the Requirement for Nucleotide Exchange. J. Bio. Chem. 274:30297-30302   DOI
7 Pan, P. W., S. H. Zhao, M. Yu, B. Liu, T. A. Xiong and K. Li. 2003. Identification of differentially expressed genes in the longissimus dorsi muscle tissue between duroc and erhualian pigs by mRNA differential display. Asian-Aust. J. Anim. Sci. 16:1066-1070   DOI
8 Lamberti, A., M. Caraglia, O. Longo, M. Marra, A. Abbruzzese and P. Arcari. 2004. The translation elongation factor 1A in tumorigenesis, signal transduction and apoptosis: Review article. Amino Acids. 26:443-448
9 Lund, A., S. M. Knudsen, H. Vissing, B. Clark and N. Tommerup. 1996. Assignment of Human Elongation Factor 1a Genes: EEF1A Maps to Chromosome 6q14 and EEF1A2 to 20q13.3. Genomics. 36:359-361   DOI   ScienceOn
10 Sanders, J., R. Raggiaschi, J. Morales and W. Moller. 1993. The human leucine zipper-containing guanine-nucleotide exchange protein elongation factor-1 delta. Biochim. Biophys. Acta. 1174:87-90   DOI   ScienceOn
11 Joseph, S. and H. F. Noller. 1998. EF-G-catalyzed translocation of anticodon stem-loop analogs of transfer RNA in the ribosome. EMBO. 17:3478-3483   DOI   ScienceOn
12 Yang, J., M. Yu, B. Liu, B. Fan, M. Zhu, T. Xiong and K. Li. 2005. Cloning and Initial Analysis of Porcine MPDU1 Gene. Asian-Aust. J. Anim. Sci. 18:1237-1241   DOI
13 Zhu, Z. M., J. B. Zhang, K. Li and S. H. Zhao. 2005. Cloning, mapping and association study with carcass traits of the porcine SDHD gene. Anim. Genet. 36:191-195   DOI   ScienceOn
14 Dever, U. E., M. J. Glynias and W. C. Merrick. 1987. Biochemistry GTP-binding domain: Three consensus sequence elements with distinct spacing. Proc. Natl. Acad. Sci. 84:1814-1818
15 Zhao, S. H., D. Nettleton, W. Liu, C. Fitzsimmons, C. W. Ernst, N. E. Raney and C. K. Tuggle. 2003. Complementary DNA macroarray analyses of differential gene expression in porcine fetal and postnatal muscle. J. Anim. Sci. 81:2179-2188