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http://dx.doi.org/10.5713/ajas.2006.347

Measurement of Microbial Protein Supply in Murrah Buffaloes (Bubalus bubalis) Using Urinary Purine Derivatives Excretion and PDC Index  

Dipu, M.T. (Animal Nutrition Division, Indian Veterinary Research Institute)
George, S.K. (Animal Nutrition Division, Indian Veterinary Research Institute)
Singh, P. (Animal Nutrition Division, Indian Veterinary Research Institute)
Verma, A.K. (Animal Nutrition Division, Indian Veterinary Research Institute)
Mehra, U.R. (Animal Nutrition Division, Indian Veterinary Research Institute)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.19, no.3, 2006 , pp. 347-355 More about this Journal
Abstract
A study was conducted to predict the rumen microbial protein production based on urinary excretion of purine derivatives in buffaloes fed a diet of wheat straw and concentrate (40:60) at four fixed levels of feed intake. (95, 80, 60 and 40% of preliminary voluntary feed intake) following experimental protocol of IAEA (Phase I). The buffaloes were allocated according to a $4{\times}4$ latin square design. The urinary allantoin, uric acid, total PD excretion (mmol/d) in treatments L-95, L-80, L-60 and L-40 was 20.13, 16.00, 12.96 and 9.17; 1.88, 2.12, 2.11 and 2.15; 22.01, 18.12, 15.07 and 11.32, respectively and were significantly (p<0.05) different among treatments except for uric acid. The rate of PD excretion (mmol/d) was positively correlated with the digestible organic matter intake. Variations were observed in PD and creatinine concentration in spot samples collected at 6-hour interval. However, daily PD:Creatinine ratio (PDC index) appears to be a reasonably good predictor of microbial-N supply. The contribution of basal purine excretion to total excretion of purine derivatives (PD) was determined in pre-fasting period followed by a fasting period of 6 d (Phase II). Daily PD and creatinine excretion (mmol/kg $W^{0.75}$) during fasting averaged 0.117 and 0.456 respectively for buffaloes. The excretion rates of PD decreased significantly (p<0.01) during fasting compare to pre-fasting period, the urinary creatinine excretion remained almost similar. Except for creatinine, plasma concentration of target parameters significantly (p<0.01) declined during fasting. Likewise, glomerular filtration rate (GFR) and renal clearance of allantoin and uric acid also decreased. Based on the PD excretion rates during fasting and at different levels of feed intake obtained in this study, a relationship between daily urinary PD excretion (Y-mmol) and microbial purine absorption (X-mmol) was developed for buffaloes as Y = 0.74X+0.117 kg $W^{0.75}$. The microbial N supply (g/kg DOMI) remained statistically similar irrespective of dietary treatment. The results showed that excretion of urinary purine derivatives is positively correlated with the levels of feed intake in Murrah buffaloes and thus, estimation of urinary purine derivatives and PDC index could be used to determine microbial nitrogen supply when there is large variation in level of feed intake.
Keywords
Buffaloes; Levels of Feed Intake; Purine Derivatives; Microbial Protein; PDC Index;
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1 Agricultural Research Council. 1984. The Nutrient Requirements of Ruminant livestock, Supplement no. 1. Slough: Commonwealth Agricultural Bureaux
2 Chen, X. B., G. Grubic, E. R. Orskov and P. Osuji. 1992. Effect of feeding frequency on diurnal variation in plasma and urinary purine derivatives in steers. Anim. Prod. 55:185-191   DOI
3 Chen, X. B. and E. R. Orskov. 2003. Research on urinary excretion of purine derivatives in ruminants: past and future. Development, standardization and validation of nuclear based technologies for estimating microbial protein supply in ruminant livestock for improving productivity. (Ed. H. P. S. Makkar) IAEA-2003, Viena
4 Chen, X. B., L. Samarweera, D. J. Kyle, E. R. Orskov and H. Abeygunawardene. 1996. Urinary excretion of purine derivatives and tissue xanthine oxidase activity in buffaloes, with special reference to differences between buffaloes and Bos taurus. Br. J. Nutr. 75:397-407   DOI   ScienceOn
5 Liang, J. B., O. Pimpa, N. Abdullah, Z. A. Jelan and J. V. Nolan. 1999. Estimation of rumen microbial protein production from urinary purine derivatives in Zebu cattle and Water buffalo, IAEA-TECDOC-1093. 35-42
6 McAllan, A. B. and R. H. Smith. 1973. Degradation of nucleic acids in rumen. Br. J. Nutr. 29:331-345   DOI   ScienceOn
7 Narayanan, S. and H. D. Appleton. 1980. Creatinine : a review. J. Clinical Chem. 26:1119-1126
8 Norton, B. W., J. B. Moran and J. V. Nolan. 1979. Nitrogen metabolism in Brahman cross, buffalo, Banteng and Shorthorn steers fed on low quality roughage. Aust. J. Agric. Res. 30:341-351   DOI
9 Ojeda, A. and O. Parra. 1999. Urinary excretion of purine derivatives as an index of microbial protein supply in crossbred (Bos indicus${\times}$Bos taurus) cattle in tropical environment. IAEA-TECDOC-1093. 59-68
10 Resines, J. E., M. J. Arin and M. T. Diez. 1992. Determination of creatinine and purine derivatives in ruminants urine by reversed phase high performance liquid chromatography. J. Chrom. 607:199-202   DOI   ScienceOn
11 Singh, M. 2004. Prediction of microbial protein production from urinary excretion of purine derivatives in crossbred cattle. M.V.Sc. Thesis. Deemed University, IVRI, Izatnagar, India
12 Sivaiah, K. 1979. Effect of feeding different levels of protein and energy on feed utilization for growth and milk production in buffaloes. Ph.D. Thesis. Kurukshetra University, India
13 Vercoe, J. E. 1976. Urinary allantoin intake and digestible organic matter intake in cattle and buffaloe. J. Agric. Sci. 86:613-615   DOI
14 AOAC. 1995. Official Methods of Analysis. 16th edn., Vol. I. Association of Official Analytical Chemists. Washington, D.C.
15 Giesecke, D., J. Balsliemke, K. H. Sudekum and M. Stangassinger. 1993. Plasma level, clearance and renal excretion of endogenous and ruminal purines in the bovine. J. Anim. Physiol. Anim. Nutr. 70:180-189   DOI
16 Hawk, P. B., B. L. Oser and W. H. Summerson. 1976. Practical Physiological Chemistry (14th edn), McGraw Hill, New York. pp. 506-509
17 Chen, X. B., A. T. Mejia, D. J. Kyle and E. R. Orskov. 1995. Evaluation of the use of the purine derivative: creatinine ratio in spot urine and plasma samples as an index of microbial protein supply in ruminants: studies in sheep. J. Agric. Sci. (Cambridge). 125:137-143   DOI   ScienceOn
18 Fujihara, T., T. Matsui and T. Harumoto. 1991. Urinary excretion of purine derivatives and blood plasma level of allantoin in sheep and goats during fasting. In: Proceedings of the Sixth International Symposium on Protein Metabolism and Nutrition, Herning, Denmark, EAAP. pp. 170-172
19 Nolan, J. V. 1999. Prediction of rumen microbial outflow based on urinary excretion derivatives. IAEA-TECDOC- 1093. 9-19
20 Snedecor, G. W. and W. G. Cochran. 1994. Statistical methods. 8th edn. Iowa State Univ., Iowa
21 Chen, X. B., D. J. Kyle, E. R. Orskov and F. D. DeB. Hovell. 1991. Renal clearance of plasma allantoin in sheep. Exp. Physiol. 76:59-65   DOI
22 Pimpa, O., J. B. Liang, J. Balcells, Z. A. Jelan and N. Abdulah. 2003. Urinary purine derivatives excretion in swamp buffaloes after duodenal purine base infusion. Anim. Feed Sci. Technol. 104:191-199   DOI   ScienceOn
23 Pimpa, O., J. B. Liang, Z. A. Jelan and N. Abdulah. 2001. Urinary excretion of duodenal purine derivatives in Kedah-kelantan cattle. Anim. Feed Sci. Technol. 92:203-214   DOI   ScienceOn
24 Verbic, J., X. B. Chen, N. A. MacLeod and E. R. Orskov. 1990. Excretion of purine derivatives by ruminants: Effects of microbial nucleic acids infusion on purine derivative excretion by steers. J. Agric. Sci. (Camb). 114:243-248   DOI
25 Soejono, M., L. M. Yusiati, S. P. S. Budhi and B. P. Widyobroto. 1999. Estimating rumen microbial protein supply for indigenous ruminants using nuclear and purine excretion techniques in Indonesia. IAEA- TECDOC- 1093. pp. 43-58
26 Balcells, J., J. A. Guada, C. Castrillo and J. Gasa. 1991. Urinary excretion of allantoin and allantoin precursors by sheep after different rates of purine infusion into duodenum. J. Agric. Sci. Cambridge. 116:309-317   DOI
27 Chen, X. B. and M. J. Gomes. 1992. Estimation of microbial protein supply to sheep and cattle based on urinary excretion of purine derivatives- An overview of the technical details. Rowett Research Institue. University of Aberdeen UK
28 Liang, J. B., M. Matsumoto, M. Ishida and B. A. Young. 1993. Urinary allantoin excretion in Malaysian cattle and buffaloes. In: Proccedings of 7th World conference on Animal Production, pp. 58-59. Alberia, Canada: University of Alberta
29 Kagiyama, K., M. Funaba, T. Iriki and M. Abe. 1996. Plasma allantoin concentration in response to changes in nutritional status of calves. Asian-Aust. J. Anim. Sci. 9:165-170   DOI
30 Shingfield, K. J. and N. W. Offer. 1998. Evaluation of the spot urine sampling technique to assess urinary purine derivative excretion in lactating dairy cows. J. Anim. Sci. 66:557-568   DOI   ScienceOn
31 Kennedy, P. M., C. S. McSweeney, D. Ffoulkes, A. John, A. C. Schlink, R. P. LeFervre and J. D. Kerr. 1992. Intake and digestion in swamp buffaloes and cattle. MARDI Res. J. 16:43-47
32 Martin-Orue, S. M., J. Balulls, J. A. Guada and M. Fondevila. 2000. Microbial nitrogen production in growing heifers: direct measurements of duodenal flow of purine bases versus urinary excretion of purine derivatives as estimation procedures. Anim. Feed Sci. Technol. 88:171-188   DOI   ScienceOn
33 Walker, D. M. and G. J. Faichney. 1964. Nitrogen balance studies with milk fed lamb. 2. The portion of Nitrogen and sulphur. Br. J. Nutr. 18:201-215   DOI   ScienceOn
34 Jayasuriya, M. C. N. 1999. Summary of the co-ordinated research project on development, standardization and validation of nuclear based technologies for estimating microbial protein supply in ruminant livestock for improving productivity. IAEA-TECDOC-1093. 1-6
35 Rys, R., A. Antoniewicz and J. Maciejewicz. 1975. Allantoin in urine as an index of microbial protein in the rumen. In: Tracer studies on non-protein nitrogen for ruminants, pp. 95-98, IAEA, Vienna
36 Ngoan Le Duc, N. X. Ba and V. K. Thanh. 2001. Estimation of rumen microbial production in swamp buffaloes. Proceeding of National workshop on swamp buffalo development, Hanoi, Vietnam
37 Chanjula, P., M. Wanapat, C. Wachirapakorn and P. Rowlinson. 2004. Effect of various levels of cassava hay on rumen ecology and digestibility in Swamp Buffaloes. Asian-Aust. J. Anim. Sci. 17:663-669   DOI
38 Moscardini, S., M. L. Haddi, B. Stefon and P. Susmel. 1999. Measurement of purine derivatives in the urine of some ruminant species. IAEA-TECDOC- 1093. 111-118
39 IAEA. 2002. Report of Final Research Co-ordination committee Meeting (IAEA), Vietnam
40 IAEA. 1999. Nuclear based technologies for estimating microbial protein supply in ruminant livestock. IAEA-TECDOC-1093, Vienna
41 IAEA. 1997. Estimation of rumen microbial protein production from purine derivatives in urine. IAEA-TECDOC-945, Vienna
42 Broderick, G. A. and N. R. Merchen. 1992. Markers for quantifying microbial protein synthesis in the rumen. J. Dairy Sci. 75:2618   DOI   ScienceOn
43 Daniels, Z. M. 1993. Purine derivatives in urine and plasma of lactating cows. M.Sc. Thesis. Aberdeen University
44 Dynes, R. A., D. A. Henry and D. G. Masters. 2003. Characterizing forages for ruminant feeding. Asian-Aust. J. Anim. Sci. 16:116-123   DOI
45 Chen, X. B., F. D. DeB. Hovell, E. R. Orskov and D. S. Brown. 1990. Excretion of purine derivatives by ruminants: effect of exogenous nucleic acid supply on purine derivatives excretion in sheep. Br. J. Nutr. 63:131-142   DOI   ScienceOn
46 Liang, J. B., M. Matsumoto and B. A. Young. 1994. Purine derivative excretion and ruminal microbial yield in Malaysian cattle and swamp buffalo. Anim. Feed Sci. Technol. 47:189- 199   DOI   ScienceOn